ID G8S3K4_ACTS5 Unreviewed; 320 AA.
AC G8S3K4;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE SubName: Full=Arabinan endo-1,5-alpha-L-arabinosidase {ECO:0000313|EMBL:AEV83570.1};
DE EC=3.2.1.99 {ECO:0000313|EMBL:AEV83570.1};
GN Name=abnA {ECO:0000313|EMBL:AEV83570.1};
GN OrderedLocusNames=ACPL_2675 {ECO:0000313|EMBL:AEV83570.1};
OS Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110).
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Actinoplanes.
OX NCBI_TaxID=134676 {ECO:0000313|EMBL:AEV83570.1, ECO:0000313|Proteomes:UP000005440};
RN [1] {ECO:0000313|Proteomes:UP000005440}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 31044 / CBS 674.73 / SE50/110
RC {ECO:0000313|Proteomes:UP000005440};
RA Schwientek P., Szczepanowski R., Kalinowski J., Klein A., Selber K.,
RA Wehmeier U.F., Stoye J., Puehler A.;
RT "The complete genome sequence of the acarbose producer Actinoplanes sp.
RT SE50/110.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834, ECO:0000256|PIRNR:PIRNR026534}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|PIRNR:PIRNR026534}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP003170; AEV83570.1; -; Genomic_DNA.
DR RefSeq; WP_014689642.1; NZ_LT827010.1.
DR AlphaFoldDB; G8S3K4; -.
DR STRING; 134676.ACPL_2675; -.
DR KEGG; ase:ACPL_2675; -.
DR PATRIC; fig|134676.3.peg.2593; -.
DR eggNOG; COG3507; Bacteria.
DR HOGENOM; CLU_009397_5_1_11; -.
DR OrthoDB; 9801455at2; -.
DR UniPathway; UPA00667; -.
DR Proteomes; UP000005440; Chromosome.
DR GO; GO:0046558; F:arabinan endo-1,5-alpha-L-arabinosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0031222; P:arabinan catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd08998; GH43_Arb43a-like; 1.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR016840; Glyco_hydro_43_endo_a_Ara-ase.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR PIRSF; PIRSF026534; Endo_alpha-L-arabinosidase; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR026534};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR026534};
KW Reference proteome {ECO:0000313|Proteomes:UP000005440};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..320
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003516493"
FT ACT_SITE 41
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-1"
FT ACT_SITE 209
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-1"
FT BINDING 41
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT BINDING 118
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT BINDING 153..156
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT BINDING 173..175
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT SITE 156
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-3"
FT SITE 279
FT /note="Important for substrate recognition"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-3"
SQ SEQUENCE 320 AA; 33941 MW; 73E51C7EC66A98F8 CRC64;
MSRFRTLMLS ALLATTVGVP PATAASYPDP GVVTGSTAAH DPSMVKTPNG GYLLATTGDG
ITLKTSSDRT RFVAAGRAFP TGTPWANDYT GGSATLWAPD ISYHSGRYLM YYAASTFGSS
RSAIFLAASP TGAAGTWTDH GKVIESTAGG NWNAIDPDLT VTPSGEWWLT LGSFWSGIKR
VRLDPATGKR ADSTMDAVAE RFVNSKSVEA PFVFRHGGYY YLFLAFDFCC RGASSTYRIM
VARSTSITGP YRDRAGTLTT AGGGTEILAS HGAIHGPGHP AVLTDSDSDV LLYHYYTSAG
DARLGINLLG WDSSAWPYVH
//
