ID G9Z0X1_9ENTR Unreviewed; 86 AA.
AC G9Z0X1;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=Sulfur carrier protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur mediator TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur transfer protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000256|HAMAP-Rule:MF_00413};
GN Name=tusA {ECO:0000256|HAMAP-Rule:MF_00413};
GN ORFNames=HMPREF0880_01123 {ECO:0000313|EMBL:EHM50305.1};
OS Yokenella regensburgei ATCC 43003.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Yokenella.
OX NCBI_TaxID=1002368 {ECO:0000313|EMBL:EHM50305.1, ECO:0000313|Proteomes:UP000003044};
RN [1] {ECO:0000313|EMBL:EHM50305.1, ECO:0000313|Proteomes:UP000003044}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43003 {ECO:0000313|EMBL:EHM50305.1,
RC ECO:0000313|Proteomes:UP000003044};
RA Weinstock G., Sodergren E., Clifton S., Fulton L., Fulton B., Courtney L.,
RA Fronick C., Harrison M., Strong C., Farmer C., Delahaunty K., Markovic C.,
RA Hall O., Minx P., Tomlinson C., Mitreva M., Hou S., Chen J., Wollam A.,
RA Pepin K.H., Johnson M., Bhonagiri V., Zhang X., Suruliraj S., Warren W.,
RA Chinwalla A., Mardis E.R., Wilson R.K.;
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC cell. Part of a sulfur-relay system required for 2-thiolation during
CC synthesis of 2-thiouridine of the modified wobble base 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC IscS and stimulates its cysteine desulfurase activity. Accepts an
CC activated sulfur from IscS, which is then transferred to TusD, and thus
CC determines the direction of sulfur flow from IscS to 2-thiouridine
CC formation. Also appears to be involved in sulfur transfer for the
CC biosynthesis of molybdopterin. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- PATHWAY: tRNA modification. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SUBUNIT: Interacts with IscS. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC {ECO:0000256|ARBA:ARBA00008984, ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHM50305.1}.
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DR EMBL; AGCL01000020; EHM50305.1; -; Genomic_DNA.
DR AlphaFoldDB; G9Z0X1; -.
DR STRING; 1002368.HMPREF0880_01123; -.
DR PATRIC; fig|1002368.3.peg.1028; -.
DR HOGENOM; CLU_165255_5_0_6; -.
DR Proteomes; UP000003044; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR CDD; cd03423; SirA; 1.
DR Gene3D; 3.30.110.40; TusA-like domain; 1.
DR HAMAP; MF_00413; Thiourid_synth_A; 1.
DR InterPro; IPR022931; Sulphur_carrier_TusA.
DR InterPro; IPR001455; TusA-like.
DR InterPro; IPR036868; TusA-like_sf.
DR PANTHER; PTHR33279:SF6; SULFUR CARRIER PROTEIN TUSA; 1.
DR PANTHER; PTHR33279; SULFUR CARRIER PROTEIN YEDF-RELATED; 1.
DR Pfam; PF01206; TusA; 1.
DR SUPFAM; SSF64307; SirA-like; 1.
DR PROSITE; PS01148; UPF0033; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413};
KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_00413}.
FT DOMAIN 17..41
FT /note="UPF0033"
FT /evidence="ECO:0000259|PROSITE:PS01148"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 24
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00413"
SQ SEQUENCE 86 AA; 9841 MW; 3F756CF3F33EBD8D CRC64;
MNEMTMSDLF SSPDHTLDAQ GLRCPEPVMM VRKTVRNMQT GETLLIIADD PATTRDIPGF
CRFMEHELVA AQTETLPYRY LLRKGH
//
