ID GDL86_ARATH Reviewed; 375 AA.
AC Q9FJ40; Q8LFP6;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE RecName: Full=GDSL esterase/lipase At5g45960;
DE EC=3.1.1.-;
DE AltName: Full=Extracellular lipase At5g45960;
DE Flags: Precursor;
GN OrderedLocusNames=At5g45960; ORFNames=K15I22.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AB016870; BAB09324.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95320.1; -; Genomic_DNA.
DR EMBL; AY084721; AAM61295.1; -; mRNA.
DR RefSeq; NP_199408.1; NM_123964.4.
DR AlphaFoldDB; Q9FJ40; -.
DR SMR; Q9FJ40; -.
DR STRING; 3702.Q9FJ40; -.
DR PaxDb; 3702-AT5G45960-1; -.
DR ProteomicsDB; 221994; -.
DR EnsemblPlants; AT5G45960.1; AT5G45960.1; AT5G45960.
DR GeneID; 834636; -.
DR Gramene; AT5G45960.1; AT5G45960.1; AT5G45960.
DR KEGG; ath:AT5G45960; -.
DR Araport; AT5G45960; -.
DR TAIR; AT5G45960; -.
DR eggNOG; ENOG502QUD3; Eukaryota.
DR HOGENOM; CLU_015101_0_1_1; -.
DR InParanoid; Q9FJ40; -.
DR OMA; HNIFLAS; -.
DR OrthoDB; 1008606at2759; -.
DR PhylomeDB; Q9FJ40; -.
DR BioCyc; ARA:AT5G45960-MONOMER; -.
DR PRO; PR:Q9FJ40; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJ40; baseline and differential.
DR Genevisible; Q9FJ40; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; SGNH hydrolase; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR PANTHER; PTHR45642; GDSL ESTERASE/LIPASE EXL3; 1.
DR PANTHER; PTHR45642:SF3; GDSL-LIKE LIPASE_ACYLHYDROLASE; 1.
DR Pfam; PF00657; Lipase_GDSL; 1.
DR SUPFAM; SSF52266; SGNH hydrolase; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..375
FT /note="GDSL esterase/lipase At5g45960"
FT /id="PRO_0000367426"
FT ACT_SITE 54
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 348
FT /evidence="ECO:0000250"
FT ACT_SITE 351
FT /evidence="ECO:0000250"
FT CARBOHYD 340
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 8
FT /note="F -> S (in Ref. 3; AAM61295)"
FT /evidence="ECO:0000305"
FT CONFLICT 298
FT /note="N -> D (in Ref. 3; AAM61295)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 375 AA; 42482 MW; 5D69953AC1137684 CRC64;
MRSHHRHFSS YVSFILFLFL FFISFSSSTS KLEPAKSEPK RKHSVSAILV FGDSTVDPGN
NNYIDTVFKC NFPPYGLDFR NKTPTGRFCN GRLVTDFIAS YIGVKENVPP YLDPNLGINE
LISGVSFASA GSGYDPLTPT ITNVIDIPTQ LEYFREYKRK LEGKMGKQEM EKHIEEAMFC
VSAGTNDFVI NYFTIPIRRK TFTIEAYQQF VISNLKQFIQ GLWKEGARKI TVAGLPPIGC
LPIVITLFSG EALTNRRCID RFSTVATNYN FLLQKQLALM QVGLAHLGSK IFYLDVYNPV
YEVIRDPRKF GFEEVFSGCC GSGYLEASFL CNPKSYVCPN TSAYVFFDSI HPSEKTYFSL
FRSLRPIYDS ILGSF
//
