ID GUN11_ORYSJ Reviewed; 500 AA.
AC Q7XTH4; A0A0P0WAM7; A3BJ50; Q0JCY5;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-JUL-2010, sequence version 3.
DT 24-JAN-2024, entry version 111.
DE RecName: Full=Endoglucanase 11;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 11;
DE AltName: Full=OsGLU4;
DE Flags: Precursor;
GN Name=GLU4; OrderedLocusNames=Os04g0443300, LOC_Os04g36610;
GN ORFNames=OsJ_023072, P0041A24.5;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=16463105; DOI=10.1007/s11103-005-2972-x;
RA Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S.,
RA Chen S.-Y.;
RT "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice,
RT affects plant internode elongation.";
RL Plant Mol. Biol. 60:137-151(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and flowers.
CC {ECO:0000269|PubMed:16463105}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; BX548155; CAE01493.1; -; Genomic_DNA.
DR EMBL; AP008210; BAF14802.2; -; Genomic_DNA.
DR EMBL; AP014960; BAS89363.1; -; Genomic_DNA.
DR EMBL; CM000144; EAZ39589.1; -; Genomic_DNA.
DR AlphaFoldDB; Q7XTH4; -.
DR SMR; Q7XTH4; -.
DR STRING; 39947.Q7XTH4; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR GlyCosmos; Q7XTH4; 2 sites, No reported glycans.
DR PaxDb; 39947-Q7XTH4; -.
DR EnsemblPlants; Os04t0443300-00; Os04t0443300-00; Os04g0443300.
DR Gramene; Os04t0443300-00; Os04t0443300-00; Os04g0443300.
DR eggNOG; ENOG502QRXS; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; Q7XTH4; -.
DR OMA; GGFQPFF; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 4.
DR Genevisible; Q7XTH4; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF54; ENDOGLUCANASE 9; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..500
FT /note="Endoglucanase 11"
FT /id="PRO_0000249288"
FT MOTIF 471..473
FT /note="Cell attachment site"
FT /evidence="ECO:0000255"
FT ACT_SITE 91
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 417
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 469
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 478
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 264
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 500 AA; 53515 MW; 50775D45C78C1B02 CRC64;
MSPSSSSSSW RALVLVAAAV LSFSGHVVVA AAAAGHPDYA DALAKSILFF QGQRSGRLPP
DQAVKWRSNS GLSDGSAANV DLTGGYYDGG DNVKFGFPMA FTTTMLSWGV VEYGGRMRGR
VLRDARDAVR WAADYLLRAA TATPGVLYVG VGDPDADHRC WERPEDMDTP RAVYSVSASS
PGSDVAAETA AALAAASLAL RAADPGYSRR LLAAARDVMA FAVRHQGKYS DHVGGDVGAY
YASYSGYQDE LLWGSAWLLW ATRNASYLDY LASLGANDGV DMFSWDNKLA GARVLLSRRA
LVNGDRRLDA FRRLAEDFIC RILPGSPSST TQYTPGGMMY KSGHANLQYV TSASFLLTTF
AKYMAVSNHT FSCQSLPVTA KTLRALARKQ VDYILGANPQ GMSYMVGYGA RFPQRIHHRG
ASMPSVAAYP AHIGCQEGFS GYFNAGGANP NVHTGAVVGG PDQHDAFPDE RGDYDRSEPT
TYTNAALVGC LAYFAGSYRS
//
