ID H0TUE9_9BRAD Unreviewed; 303 AA.
AC H0TUE9;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=Haloalkane dehalogenase {ECO:0000256|ARBA:ARBA00012065, ECO:0000256|HAMAP-Rule:MF_01231};
DE EC=3.8.1.5 {ECO:0000256|ARBA:ARBA00012065, ECO:0000256|HAMAP-Rule:MF_01231};
GN Name=dhaA {ECO:0000256|HAMAP-Rule:MF_01231,
GN ECO:0000313|EMBL:CCE10087.1};
GN ORFNames=BRAS3843_380038 {ECO:0000313|EMBL:CCE10087.1};
OS Bradyrhizobium sp. STM 3843.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=551947 {ECO:0000313|EMBL:CCE10087.1, ECO:0000313|Proteomes:UP000002686};
RN [1] {ECO:0000313|Proteomes:UP000002686}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=STM3843 {ECO:0000313|Proteomes:UP000002686};
RX PubMed=24704842; DOI=10.3390/genes3010035;
RA Mornico D., Miche L., Bena G., Nouwen N., Vermeglio A., Vallenet D.,
RA Smith A.A., Giraud E., Medigue C., Moulin L.;
RT "Comparative genomics of aeschynomene symbionts: insights into the
RT ecological lifestyle of nod-independent photosynthetic bradyrhizobia.";
RL Genes (Basel) 3:35-61(2011).
CC -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC halogenated aliphatic compounds, leading to the formation of the
CC corresponding primary alcohols, halide ions and protons.
CC {ECO:0000256|HAMAP-Rule:MF_01231}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01231};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245, ECO:0000256|HAMAP-
CC Rule:MF_01231}.
CC -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 2
CC subfamily. {ECO:0000256|ARBA:ARBA00007213, ECO:0000256|HAMAP-
CC Rule:MF_01231}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCE10087.1}.
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DR EMBL; CAFK01000283; CCE10087.1; -; Genomic_DNA.
DR RefSeq; WP_008972598.1; NZ_CAFK01000283.1.
DR AlphaFoldDB; H0TUE9; -.
DR ESTHER; 9brad-h0tue9; Haloalkane_dehalogenase-HLD2.
DR OrthoDB; 9812774at2; -.
DR Proteomes; UP000002686; Unassembled WGS sequence.
DR GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR HAMAP; MF_01231; Haloalk_dehal_type2; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR000639; Epox_hydrolase-like.
DR InterPro; IPR023594; Haloalkane_dehalogenase_2.
DR PANTHER; PTHR43329; EPOXIDE HYDROLASE; 1.
DR PANTHER; PTHR43329:SF74; HALOALKANE DEHALOGENASE; 1.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR PRINTS; PR00412; EPOXHYDRLASE.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_01231, ECO:0000313|EMBL:CCE10087.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002686}.
FT DOMAIN 33..145
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000259|Pfam:PF00561"
FT ACT_SITE 103
FT /note="Nucleophile"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01231"
FT ACT_SITE 127
FT /note="Proton donor"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01231"
FT ACT_SITE 272
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01231"
SQ SEQUENCE 303 AA; 32859 MW; F76E28A54AB45386 CRC64;
MTETAQSGLH HLDVLDTTMA YREMGRTDAP VALFLHGNPT SSYIWRNIMP LVAPVARCVA
PDLVGFGQSG KPDIGYRFFD QARYLDAFVD GLGLRSAYLV AQDWGTALAF HLAARRPSLV
RGLAFMEFIR PMADWSDFDP THGVREQTFR RFRTAGEGEA MILEGNAFVE RVLPGGVVRA
LSEDEMAAYR APFATPESRR PTLVLPRELP IGGEPADVDQ ALRIAHAALA ASRYPKLLFV
GDPGALVSPS RAKEFAASLH NIAVIPLGAG RHFLQEDHPE AIGRSVAGWI AGIETATALG
NAA
//
