ID H0X7V6_OTOGA Unreviewed; 880 AA.
AC H0X7V6;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=Beta-mannosidase {ECO:0000256|ARBA:ARBA00015707};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Lysosomal beta A mannosidase {ECO:0000256|ARBA:ARBA00032581};
DE AltName: Full=Mannanase {ECO:0000256|ARBA:ARBA00033445};
GN Name=MANBA {ECO:0000313|Ensembl:ENSOGAP00000011520.2};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000011520.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000011520.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Exoglycosidase that cleaves the single beta-linked mannose
CC residue from the non-reducing end of all N-linked glycoprotein
CC oligosaccharides. {ECO:0000256|ARBA:ARBA00003150}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000256|ARBA:ARBA00004371}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401}.
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DR EMBL; AAQR03127025; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127026; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127027; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127028; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127029; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127030; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127031; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127032; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127033; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03127034; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; H0X7V6; -.
DR STRING; 30611.ENSOGAP00000011520; -.
DR Ensembl; ENSOGAT00000012855.2; ENSOGAP00000011520.2; ENSOGAG00000012852.2.
DR eggNOG; KOG2230; Eukaryota.
DR GeneTree; ENSGT00390000001670; -.
DR HOGENOM; CLU_005015_3_1_1; -.
DR InParanoid; H0X7V6; -.
DR OMA; KRQWKGP; -.
DR TreeFam; TF105723; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 3.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lysosome {ECO:0000256|ARBA:ARBA00023228};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..880
FT /note="Beta-mannosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003544541"
FT DOMAIN 343..507
FT /note="Glycoside hydrolase family 2 catalytic"
FT /evidence="ECO:0000259|Pfam:PF02836"
FT DOMAIN 703..795
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 800..879
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 880 AA; 101462 MW; B1C6AC4C806DFE9A CRC64;
MHLYLLLLLA LWLEGSTAEE RSYGLGGRWS IRSGNGSLEL SGEVPGCVHS ALFQQGLIQD
PYYRFNDLNY RWISLDNWTY SKTFKIPFDI SKWQKVNLIF EGVDTVSKIL FNNITIGATD
NMFNRYSFDI TSLVRDVNCL EVRFQSALSY AAQRSRAHTR YRVPPECPPS VQKGECHVNF
VRKEQCSFSW DWGPSFPTQG IWKDVRIEAY NICHLNYFTF SPTYDNSARE WNLEIESSFD
VVSSKSVGGK VIVSIPKLQT NQIYNLELQP GERIVELFVK INKNVTVETW WPHGHGNQTG
YNMTVLFELD GGVTIEKSAK VYFRTVELIE EPIKGSPGLS FYFKINGFPI FLKGSNWIPA
DAFQDRVTSD VLRLLLQSAV DANMNTLRVW GGGIYEQDEF YQLCDELGIM IWQDFMFACA
LYPVDRDFID SVRAEVAYQI RRLKSHPSII IWSGNNENEA ALMTNWYHIS PVDLHTYIND
YVTLYVKNIR EMVLAGDKSR PFIISSPTNG VETIAEGWVS HDPYSNYFGD VHFYDYFSDC
WDWKVFPKAR FASEYGYQSW PSFSTLEKKV SSKKDWSFNS KFSLHRQHHE DGNKQMLHQA
QLHFKLPQST DPLRAFKDTI YLTQVMQAQC VKTETEFYRR SRSEIVNKKG HTMGALYWQL
NDIWQAPSWA SLEYGGKWKM LHYFARRFFA PLLPVGFEDQ SVFHVYGVSD LHSDLRVMLT
VRVHTWSSLE PLCSLATKGF VIRAGKAILL YKEPVSSLLR RCGHCTRKSC VVSFYLSNDL
ELLSPINHHF LSSLKEAVGL RRAHITAIIS QQGDTFVFDL ETSAVAPFVW LDVGSIPGRF
SDNGFLMTEK TRSISFYPWK PTSKSELERS LRVTSLTDIY
//
