ID H0Y2B3_OTOGA Unreviewed; 499 AA.
AC H0Y2B3;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE RecName: Full=Retinal dehydrogenase 2 {ECO:0000256|ARBA:ARBA00041052};
DE EC=1.2.1.36 {ECO:0000256|ARBA:ARBA00039134};
DE AltName: Full=Aldehyde dehydrogenase family 1 member A2 {ECO:0000256|ARBA:ARBA00041636};
DE AltName: Full=Retinaldehyde-specific dehydrogenase type 2 {ECO:0000256|ARBA:ARBA00043059};
GN Name=ALDH1A2 {ECO:0000313|Ensembl:ENSOGAP00000022506.1};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000022506.1, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000022506.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) + retinal = 2 H(+) + NADH + retinoate;
CC Xref=Rhea:RHEA:16177, ChEBI:CHEBI:15035, ChEBI:CHEBI:15036,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.2.1.36;
CC Evidence={ECO:0000256|ARBA:ARBA00036070};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16178;
CC Evidence={ECO:0000256|ARBA:ARBA00036070};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-13,14-dihydroretinal + H2O + NAD(+) = all-
CC trans-13,14-dihydroretinoate + 2 H(+) + NADH; Xref=Rhea:RHEA:75119,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:194182, ChEBI:CHEBI:194183;
CC Evidence={ECO:0000256|ARBA:ARBA00035998};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:75120;
CC Evidence={ECO:0000256|ARBA:ARBA00035998};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-retinal + H2O + NAD(+) = all-trans-retinoate + 2
CC H(+) + NADH; Xref=Rhea:RHEA:42080, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17898, ChEBI:CHEBI:35291,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.2.1.36;
CC Evidence={ECO:0000256|ARBA:ARBA00035919};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42081;
CC Evidence={ECO:0000256|ARBA:ARBA00035919};
CC -!- PATHWAY: Cofactor metabolism; retinol metabolism.
CC {ECO:0000256|ARBA:ARBA00004891}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00009986, ECO:0000256|RuleBase:RU003345}.
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DR EMBL; AAQR03025375; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03025376; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03025377; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03025378; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03025379; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03025380; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03025381; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03025382; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03025383; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; H0Y2B3; -.
DR Ensembl; ENSOGAT00000024803.1; ENSOGAP00000022506.1; ENSOGAG00000007796.2.
DR GeneTree; ENSGT00940000158898; -.
DR HOGENOM; CLU_005391_0_1_1; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016620; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR CDD; cd07141; ALDH_F1AB_F2_RALDH1; 1.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016163; Ald_DH_C.
DR InterPro; IPR016160; Ald_DH_CS_CYS.
DR InterPro; IPR029510; Ald_DH_CS_GLU.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH_dom.
DR PANTHER; PTHR11699; ALDEHYDE DEHYDROGENASE-RELATED; 1.
DR PANTHER; PTHR11699:SF102; RETINAL DEHYDROGENASE 2; 1.
DR Pfam; PF00171; Aldedh; 1.
DR SUPFAM; SSF53720; ALDH-like; 1.
DR PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE 3: Inferred from homology;
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Oxidoreductase {ECO:0000256|RuleBase:RU003345};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225}.
FT DOMAIN 27..490
FT /note="Aldehyde dehydrogenase"
FT /evidence="ECO:0000259|Pfam:PF00171"
FT ACT_SITE 267
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10007"
SQ SEQUENCE 499 AA; 54722 MW; 9245A91773F1742A CRC64;
MASLHLLPSP TPNLEIKYTK IFINNEWQNS ESGRVFPVYN PATGEQVCEV QEADKADIDK
AVQAARLAFS LGSVWRRMDA SERGRLLDKL ADLVERDRAV LATMECLNGG KPFLQAFYVD
LQGVIKTLRY YAGWADKIHG MTIPVDGDYF TFTRHEPIGV CGQIIPWNFP LLMFAWKIAP
ALCCGNTVVI KPAEQTPLSA LYMGALIKEA GFPPGVINIL PGYGPTAGAA IASHVGIDKI
AFTGSTEVGK LIQEAAGRSN LKRVTLELGG KSPNIIFADA DLDYAVEQAH QGVFFNQGQC
CTAGSRIFVE ESIYEEFVRR SVERAKRRIV GSPFDPTTEQ GPQIDKKQYN KILELIQSGV
AEGATLECGG KGLGRKGFFI EPTVFSNVTD DMRIAKEEIF GPVQEILRFK TMDEVIERAN
NSDFGLVAAV FTNDINKALT VSSAMQAGTV WINCYNALNA QSPFGGFKMS GNGREMGEFG
LREYSEVKTV TVKIPQKNS
//
