ID H2JF73_9CLOT Unreviewed; 354 AA.
AC H2JF73;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 21-MAR-2012, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=Aldose 1-epimerase {ECO:0000256|ARBA:ARBA00013185, ECO:0000256|PIRNR:PIRNR005096};
DE EC=5.1.3.3 {ECO:0000256|ARBA:ARBA00013185, ECO:0000256|PIRNR:PIRNR005096};
GN ORFNames=Clo1100_1202 {ECO:0000313|EMBL:AEY65453.1};
OS Clostridium sp. BNL1100.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=755731 {ECO:0000313|EMBL:AEY65453.1, ECO:0000313|Proteomes:UP000007324};
RN [1] {ECO:0000313|EMBL:AEY65453.1, ECO:0000313|Proteomes:UP000007324}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BNL1100 {ECO:0000313|EMBL:AEY65453.1,
RC ECO:0000313|Proteomes:UP000007324};
RX PubMed=23209234; DOI=10.1128/JB.01908-12;
RA Li L.L., Taghavi S., Izquierdo J.A., van der Lelie D.;
RT "Complete Genome Sequence of Clostridium sp. Strain BNL1100, a Cellulolytic
RT Mesophile Isolated from Corn Stover.";
RL J. Bacteriol. 194:6982-6983(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose = beta-D-glucose; Xref=Rhea:RHEA:10264,
CC ChEBI:CHEBI:15903, ChEBI:CHEBI:17925; EC=5.1.3.3;
CC Evidence={ECO:0000256|ARBA:ARBA00001614,
CC ECO:0000256|PIRNR:PIRNR005096};
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000256|ARBA:ARBA00005028, ECO:0000256|PIRNR:PIRNR005096}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the aldose epimerase family.
CC {ECO:0000256|ARBA:ARBA00006206, ECO:0000256|PIRNR:PIRNR005096}.
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DR EMBL; CP003259; AEY65453.1; -; Genomic_DNA.
DR RefSeq; WP_014312835.1; NC_016791.1.
DR AlphaFoldDB; H2JF73; -.
DR STRING; 755731.Clo1100_1202; -.
DR KEGG; clb:Clo1100_1202; -.
DR PATRIC; fig|755731.4.peg.1198; -.
DR eggNOG; COG2017; Bacteria.
DR HOGENOM; CLU_031753_1_1_9; -.
DR OrthoDB; 9779408at2; -.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000007324; Chromosome.
DR GO; GO:0004034; F:aldose 1-epimerase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd09019; galactose_mutarotase_like; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR InterPro; IPR018052; Ald1_epimerase_CS.
DR InterPro; IPR015443; Aldose_1-epimerase.
DR InterPro; IPR008183; Aldose_1/G6P_1-epimerase.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR047215; Galactose_mutarotase-like.
DR InterPro; IPR014718; GH-type_carb-bd.
DR PANTHER; PTHR10091; ALDOSE-1-EPIMERASE; 1.
DR PANTHER; PTHR10091:SF0; GALACTOSE MUTAROTASE; 1.
DR Pfam; PF01263; Aldose_epim; 1.
DR PIRSF; PIRSF005096; GALM; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR PROSITE; PS00545; ALDOSE_1_EPIMERASE; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|PIRNR:PIRNR005096};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|PIRNR:PIRNR005096};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}.
FT ACT_SITE 180
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005096-1"
FT ACT_SITE 319
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005096-1"
FT BINDING 80..81
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000256|PIRSR:PIRSR005096-3"
FT BINDING 180..182
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000256|PIRSR:PIRSR005096-3"
FT BINDING 252
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000256|PIRSR:PIRSR005096-2"
SQ SEQUENCE 354 AA; 39764 MW; 0A86D3CC2B412788 CRC64;
MSITKKNFAK MPDGTQVDIY TLKNSNNMSI DILNYGGTIV SILMPDKNGK IADIALGFDD
IKRYMENDAY MGALIGRHGN RIEGAEFELN GKVYELVKND GNNHLHGGTI GFHNVVWNAE
IQEEKGVQKL VLTHTSPDGE ENYPGNLNVK VEYSLSENNE LKIDYNAVSD KDTVVNLTNH
AYFNLSGHDS GDVLKHQIYI NADKFTPVND ECIPTGEIRD VKGTVMDFTK LSSFSEGISS
GDEQIVYGLG YDHNWVLNTE GNINEVACEL YEPESGRAMQ VFTTKPGIQF YSGNHLNEKV
ELIGKNNTVY NKRTGLCLET QFFPNAMKHK NFPSPILKAN ETYHYVTIYK FFNK
//
