ID H2LIZ3_ORYLA Unreviewed; 1262 AA.
AC H2LIZ3;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN Name=iars1 {ECO:0000313|Ensembl:ENSORLP00000005976.2};
OS Oryzias latipes (Japanese rice fish) (Japanese killifish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=8090 {ECO:0000313|Ensembl:ENSORLP00000005976.2, ECO:0000313|Proteomes:UP000001038};
RN [1] {ECO:0000313|Ensembl:ENSORLP00000005976.2, ECO:0000313|Proteomes:UP000001038}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000005976.2,
RC ECO:0000313|Proteomes:UP000001038};
RX PubMed=17554307; DOI=10.1038/nature05846;
RA Kasahara M., Naruse K., Sasaki S., Nakatani Y., Qu W., Ahsan B., Yamada T.,
RA Nagayasu Y., Doi K., Kasai Y., Jindo T., Kobayashi D., Shimada A.,
RA Toyoda A., Kuroki Y., Fujiyama A., Sasaki T., Shimizu A., Asakawa S.,
RA Shimizu N., Hashimoto S., Yang J., Lee Y., Matsushima K., Sugano S.,
RA Sakaizumi M., Narita T., Ohishi K., Haga S., Ohta F., Nomoto H., Nogata K.,
RA Morishita T., Endo T., Shin-I T., Takeda H., Morishita S., Kohara Y.;
RT "The medaka draft genome and insights into vertebrate genome evolution.";
RL Nature 447:714-719(2007).
RN [2] {ECO:0000313|Ensembl:ENSORLP00000005976.2}
RP IDENTIFICATION.
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000005976.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR AlphaFoldDB; H2LIZ3; -.
DR Ensembl; ENSORLT00000005977.2; ENSORLP00000005976.2; ENSORLG00000004747.2.
DR eggNOG; KOG0434; Eukaryota.
DR GeneTree; ENSGT00550000074921; -.
DR HOGENOM; CLU_001493_1_1_1; -.
DR Proteomes; UP000001038; Chromosome 5.
DR Bgee; ENSORLG00000004747; Expressed in ovary and 14 other cell types or tissues.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000001038}.
FT DOMAIN 17..641
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 696..852
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1262 AA; 144018 MW; 08A5DF0C207FFAB1 CRC64;
MVEPVPESIN FPSEEEKILE FWQKKDCFQE CLKQSKNRPR YTFYDGPPFA TGLPHYGHIL
AGTIKDIVTR FAHQSGFYVE RRFGWDCHGL PVEYEIDKTL GIKGPEDVAK MGIAEYNKQC
RNIVMRYSHE WETSVKRMGR WIDFKNDYKT LYPWFMETVW WVFKQLYDKG MVYRGVKVMP
FSTACNTPLS NFESHQNYKD VQDPSVIVNF PLVEDKDVSL IAWTTTPWTL PSNLALCVNP
DYLYVKVKGE ECFSLGCHGI LFPGKNLKGK KYKPLFQYFS KCGEKGAFQV VLDNYVKEEE
GTGVVHQAPY FGADDYRVCA EYNIIQRDQP PICPVDASGC FTSEVTDFAG QYVKDADKNI
IKWLKENGRL VNFSTFKHSY PFCWRSDTPL IYKAVPSWFV RVEHMVEKLL DCNGKCYWVP
EFVREKRFGN WLRDARDWAI SRNRYWGTPI PLWVSDDFQE VVCVGSIAEL EELTGVKVAD
LHRESIDSLT IPSRCGKGVL RRVTEVFDCW FESGSMPYAQ VHYPFENKKE FEESFPADFI
AEGIDQTRGC SLVDTNSYFL SFFIFVCHCR FYTLLVLSTA LFGKPPFKNV IVNGLVLASD
GQKMSKRKKN YPDPALIVQN YGADALRLYL INSPVVRAEN LRFKEEGVRD ILKDVFLPWY
NAYRFLVQNV QRLQKEDGIG FLYNENTAKQ SDNIMDKWIQ SFTQSLIQFF KAEMDAYRLY
TVVPRLVKFV DMLTNWYVRT NRRRLKGESG TEDCLWALET LFSVLFSMCR LMAPFTPFIT
EMMYQNLCHL IDPASVEEKD ASSIHYLMLP QVRESLIDKR IECAVSQMQS VIELGRVIRD
RKTLPVKYPL KEVVVIHQDP EALKDIQSLQ QYILEELNVR QLTLSTDKDK YGIRLRAEPD
HMVLGKRLKG AFKAVTASIK ELTSEQLEAF QKTGRIMVNE HELHEEDLRL MYTFDQSSGS
TAQYEAHSDA QVLVLLDVTP DQSMVDEGVA REVINRIQKL RKKGHLVPSD EITVHYHCQP
EGEYLASVIQ AHTDFILGTT KAPLLPYPVP KSASIIIAET TQLKGSDLEL TIVKGSSVHQ
ASLNGPACAY VNARVKVEGV ILLENPKGDN RLDLDRLQTV CSSVFGTNNS RLRFFSGGTE
LTSKMDLLAL SGKTLAVTSG PSSPGPAPTA NTPLCPYVNL VLCNAQPAEC QTGEVGTLLL
MNPVGHNALN YPGLLSETGK VFGLRSRRLK LYLDQDFTEE SSIKSLDTKT LFVRVLPTTA
NA
//
