ID H2M8Z8_ORYLA Unreviewed; 1216 AA.
AC H2M8Z8;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 73.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN Name=DHX8 {ECO:0000313|Ensembl:ENSORLP00000014975.3};
OS Oryzias latipes (Japanese rice fish) (Japanese killifish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=8090 {ECO:0000313|Ensembl:ENSORLP00000014975.3, ECO:0000313|Proteomes:UP000001038};
RN [1] {ECO:0000313|Ensembl:ENSORLP00000014975.3, ECO:0000313|Proteomes:UP000001038}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000014975.3,
RC ECO:0000313|Proteomes:UP000001038};
RX PubMed=17554307; DOI=10.1038/nature05846;
RA Kasahara M., Naruse K., Sasaki S., Nakatani Y., Qu W., Ahsan B., Yamada T.,
RA Nagayasu Y., Doi K., Kasai Y., Jindo T., Kobayashi D., Shimada A.,
RA Toyoda A., Kuroki Y., Fujiyama A., Sasaki T., Shimizu A., Asakawa S.,
RA Shimizu N., Hashimoto S., Yang J., Lee Y., Matsushima K., Sugano S.,
RA Sakaizumi M., Narita T., Ohishi K., Haga S., Ohta F., Nomoto H., Nogata K.,
RA Morishita T., Endo T., Shin-I T., Takeda H., Morishita S., Kohara Y.;
RT "The medaka draft genome and insights into vertebrate genome evolution.";
RL Nature 447:714-719(2007).
RN [2] {ECO:0000313|Ensembl:ENSORLP00000014975.3}
RP IDENTIFICATION.
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000014975.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; H2M8Z8; -.
DR STRING; 8090.ENSORLP00000014975; -.
DR Ensembl; ENSORLT00000014976.3; ENSORLP00000014975.3; ENSORLG00000011951.3.
DR eggNOG; KOG0922; Eukaryota.
DR GeneTree; ENSGT00940000155510; -.
DR HOGENOM; CLU_001832_2_3_1; -.
DR InParanoid; H2M8Z8; -.
DR TreeFam; TF300509; -.
DR Proteomes; UP000001038; Chromosome 19.
DR Bgee; ENSORLG00000011951; Expressed in gastrula and 14 other cell types or tissues.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0000390; P:spliceosomal complex disassembly; IBA:GO_Central.
DR CDD; cd17971; DEXHc_DHX8; 1.
DR CDD; cd21691; GH2-like_DHX8; 1.
DR CDD; cd05684; S1_DHX8_helicase; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044762; DHX8/Prp22_DEXHc.
DR InterPro; IPR049588; DHX8_GH2-like.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049621; S1_DHX8_helicase.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF85; ATP-DEPENDENT RNA HELICASE DHX8; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50126; S1; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000001038}.
FT DOMAIN 261..332
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT DOMAIN 571..734
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 752..932
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 119..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 179..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 209..256
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 333..360
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1216 AA; 138169 MW; BD5C3F3CB63919A8 CRC64;
MISYQIPRIV FPFTPEVIKY LTCPLSATIS EQKQGSWRMA DIGLEELSQL EYLSLVSKVC
TELDNHLGIN DKDLAEFVIS LAEKQPAFDG FKAVLLENGA EFTDSLISNL LRLIQTMQPP
SKASMSKVSE ASVKPKSEKD RLKEMFPALC RPDDPVPKQH LDEDDVKVAA DAMKELEMFM
PSVSGAESKG GKTKSDKSRH RSRSRSRDRH RDRDRDRGKR REKSSRWGDR SPSPRKEQDR
DGDRWKDKHV DRPPPEEPSV GDIYNGKITS IMQFGCFVQL EGLRKRWEGL VHISELRREG
RVANVADVVS KGQRVKVKVL SFTGSKTSLS MKDVDQDTGE DLNPNRRRNV GPEGEEVSMR
NPDRPSNLNL GHAPEMEQDD TLERKRLTKI SDPEKWEIKQ MIAANVLSKE EFPDFDDETG
ILPKVDDEED EDLEIELVEE EPPFLRGHTK QSMDMSPVKI VKNPDGSLSQ AAMMQSALAK
ERRELKQAAR EAEMDSIPMG LNKHWVDPLP DVDGRQIAAN MRGIGMMPND IPEWKKHAFG
GNKASYGKKT QLSILEQRES LPIYKLKEQL VQAVHDNQIL IVIGETGSGK TTQITQYLAE
AGYTARGKIG CTQPRRVAAM SVAKRVSEEY GCCLGQEVGY TIRFEDCTSP ETVIKYMTDG
MLLRECLIDS ELGQYAIIML DEAHERTIHT DVLFGLLKKT VQKRTDMKLI VTSATLDAVK
FSQYFYEAPI FTIPGRTYPV EVLYTKEPET DYLDASLITV MQIHLTEPPG DILVFLTGQE
EIDTACEILY ERMKSLGPDV PELIILPVYS ALPSEMQTRI FDPAPPGSRK VVIATNIAET
SLTIDGIYYV VDPGFVKQKV YNSKTGIDQL VVTPISQAQA KQRAGRAGRT GPGKCYRLYT
ERAYRDEMLT TNVPEIQRTN LASTVLSLKA MGINDLLSFD FMDAPPMETL ITAMEQLYTL
GALDDEGLLT RLGRRMAEFP LEPMLCKMLI MSVHLGCSEE MLTIVSMLSV QNVFYRPKDK
QALADQKKAK FHQPEGDHLT LLAVYNSWKN NKFSNPWCYE NFIQARSLRR AQDIRKQMLG
IMDRHKLDVV SCGKATVRVQ KAICSGFFRN AAKKDPQEGY RTLIDQQVVY IHPSSALFNR
QPEWVVYHEL VLTTKEYMRE VTTIDPRWLV EFAPAFFKVS DPTRLSKQKK QQRLEPLYNR
YEEPNAWRIS RAFRRR
//
