ID H3RIV1_PANSE Unreviewed; 246 AA.
AC H3RIV1;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000256|HAMAP-Rule:MF_01001};
DE Short=UDP-ManNAcA transferase {ECO:0000256|HAMAP-Rule:MF_01001};
DE EC=2.4.1.180 {ECO:0000256|HAMAP-Rule:MF_01001};
GN Name=wecG {ECO:0000256|HAMAP-Rule:MF_01001};
GN Synonyms=rffM {ECO:0000256|HAMAP-Rule:MF_01001};
GN ORFNames=CKS_4426 {ECO:0000313|EMBL:EHT98809.1}, DSJ_22155
GN {ECO:0000313|EMBL:ARF52036.1};
OS Pantoea stewartii subsp. stewartii DC283.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea.
OX NCBI_TaxID=660596 {ECO:0000313|EMBL:EHT98809.1, ECO:0000313|Proteomes:UP000005050};
RN [1] {ECO:0000313|EMBL:EHT98809.1, ECO:0000313|Proteomes:UP000005050}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DC283 {ECO:0000313|EMBL:EHT98809.1,
RC ECO:0000313|Proteomes:UP000005050};
RX PubMed=22111898; DOI=10.1111/j.1365-2958.2011.07926.x;
RA Wang X., Yang F., von Bodman S.B.;
RT "The genetic and structural basis of two distinct terminal side branch
RT residues in stewartan and amylovoran exopolysaccharides and their potential
RT role in host adaptation.";
RL Mol. Microbiol. 83:195-207(2012).
RN [2] {ECO:0000313|EMBL:EHT98809.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DC283 {ECO:0000313|EMBL:EHT98809.1};
RA Biehl B.S., Ding Y., Dugan-Rocha S.P., Gibbs R.A., Glasner J.D., Kovar C.,
RA Muzny D.M., Neeno-Eckwall E.C., Perna N.T., Qin X., von Bodman S.B.,
RA Weinstock G.M.;
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ARF52036.1, ECO:0000313|Proteomes:UP000192380}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DC283 {ECO:0000313|EMBL:ARF52036.1,
RC ECO:0000313|Proteomes:UP000192380};
RA Duong D.A., Stevens A.M., Jensen R.V.;
RT "Complete Genome Assembly of Pantoea stewartii subsp. stewartii DC283, a
RT Corn Pathogen.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC the second lipid-linked intermediate involved in enterobacterial common
CC antigen (ECA) synthesis. {ECO:0000256|HAMAP-Rule:MF_01001}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01001};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000256|HAMAP-Rule:MF_01001}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC {ECO:0000256|HAMAP-Rule:MF_01001}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP017581; ARF52036.1; -; Genomic_DNA.
DR EMBL; AHIE01000033; EHT98809.1; -; Genomic_DNA.
DR RefSeq; WP_006121441.1; NZ_CP017581.1.
DR AlphaFoldDB; H3RIV1; -.
DR STRING; 660596.DSJ_22155; -.
DR GeneID; 61253291; -.
DR KEGG; pstw:DSJ_22155; -.
DR PATRIC; fig|660596.6.peg.4220; -.
DR eggNOG; COG1922; Bacteria.
DR OrthoDB; 9808602at2; -.
DR UniPathway; UPA00566; -.
DR Proteomes; UP000005050; Unassembled WGS sequence.
DR Proteomes; UP000192380; Chromosome.
DR GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR HAMAP; MF_01001; WecG_RffM; 1.
DR InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR InterPro; IPR004629; WecG_TagA_CpsF.
DR NCBIfam; TIGR00696; wecG_tagA_cpsF; 1.
DR PANTHER; PTHR34136; -; 1.
DR PANTHER; PTHR34136:SF1; UDP-N-ACETYL-D-MANNOSAMINURONIC ACID TRANSFERASE; 1.
DR Pfam; PF03808; Glyco_tran_WecG; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|HAMAP-Rule:MF_01001,
KW ECO:0000313|EMBL:EHT98809.1};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_01001, ECO:0000313|EMBL:EHT98809.1}.
SQ SEQUENCE 246 AA; 27715 MW; 00B66E3558225A99 CRC64;
MSTHTGTPRY EIRGITLYGF DSMTAFVDFL MPPSGIRTGT LVAINAEKVL SAERDAALSA
QIQQAEFNYP DGISIVRSIR KKYPQASVQR IAGADLWEAL MQRAGREGTP VFLIGGREAV
LDETCAKLRR QWNVNIVGAQ NGYFVPSERE ALCARIAASG AKLVTVAMGS PRQELFMADC
QRQWPEALYM GVGGTYDVFT GHVKRAPVFW QKLGLEWLYR LLVQPSRLRR QLKLVKYLRY
HWRGDL
//
