ID H6N5Y5_MYCHN Unreviewed; 657 AA.
AC H6N5Y5;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 2.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN Name=gyrB {ECO:0000313|EMBL:AEW44900.2};
GN OrderedLocusNames=MHC_00170 {ECO:0000313|EMBL:AEW44900.2};
OS Mycoplasma haemocanis (strain Illinois).
OC Bacteria; Mycoplasmatota; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=1111676 {ECO:0000313|EMBL:AEW44900.2, ECO:0000313|Proteomes:UP000009135};
RN [1] {ECO:0000313|EMBL:AEW44900.2, ECO:0000313|Proteomes:UP000009135}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Illinois {ECO:0000313|EMBL:AEW44900.2,
RC ECO:0000313|Proteomes:UP000009135};
RX PubMed=22374945; DOI=10.1128/JB.06781-11;
RA do Nascimento N.C., Guimaraes A.M., Santos A.P., Sanmiguel P.J.,
RA Messick J.B.;
RT "Complete genome sequence of Mycoplasma haemocanis strain Illinois.";
RL J. Bacteriol. 194:1605-1606(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP003199; AEW44900.2; -; Genomic_DNA.
DR AlphaFoldDB; H6N5Y5; -.
DR STRING; 1111676.MHC_00170; -.
DR KEGG; mhe:MHC_00170; -.
DR HOGENOM; CLU_006146_4_1_14; -.
DR Proteomes; UP000009135; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd16928; HATPase_GyrB-like; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR002288; DNA_gyrase_B_C.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF00986; DNA_gyraseB_C; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:AEW44900.2};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000009135};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 440..555
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 657 AA; 74047 MW; 4F737644C44F0EFF CRC64;
MESTGNDPED LGQKGNYTDS SIKVLEGLEA VRERPGMYIG STDSKGLHHL IWEVLDNSVD
EVLAGHANEV VLTLKPDHVI SINDNGRGIP TGINPQTNIS NLITVFTILH AGGKFDNNSY
KTSGGLHGVG STCVNALSEF MEVTVCRNGE EHFVSFKNGG KIDKEPTLVS KVPVEKTGTR
VTWKPDFSIF DKSDYDIGLI ETRLERLAYL NKGRKFVFDN QISKEVKEFF FKDGISDWVK
NLNNSRKAIN DVIFLKEDGT VKNRRSPDIQ NPISISCAFQ YTLGDSPIVY SFCNNIYTAS
GGTHLESFKD GILSCIRERS LEAKLIKESI DLVKSDILAG LTAIVSLNYS NPEYSGQTKE
VLSNIEIKSF IREKVEELFG RFLEENSDQC KAILQRVDQE RNLRLKVEMA RQTDRKLALE
GFMSFAGKLA DCTTKSVDFS ELYVVEGDSA GGSAKSARNR EYQAILPIKG KLLNVWKKTN
TSSIVENEEI KSLISSIGCA YGDKFDKEKL RYNKVIIMTD ADVDGSHIRI LLLTFIYRFM
RPLIEEGHVY IAQPPLYRAF TNKEVVYLFD DKKKDEFMAD KGKSKSWEIS RFKGLGEMSP
EQLWQTTMNP EERIICKVTV EDMEQVTGIF DDLMGKKVHP RTEFILANYS NIRNLDI
//
