ID H6NFB7_9BACL Unreviewed; 864 AA.
AC H6NFB7;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=PM3016_2644 {ECO:0000313|EMBL:AFC29526.1};
OS Paenibacillus mucilaginosus 3016.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1116391 {ECO:0000313|EMBL:AFC29526.1, ECO:0000313|Proteomes:UP000007523};
RN [1] {ECO:0000313|EMBL:AFC29526.1, ECO:0000313|Proteomes:UP000007523}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3016 {ECO:0000313|EMBL:AFC29526.1,
RC ECO:0000313|Proteomes:UP000007523};
RX PubMed=22535950; DOI=10.1128/JB.00323-12;
RA Ma M., Wang Z., Li L., Jiang X., Guan D., Cao F., Chen H., Wang X.,
RA Shen D., Du B., Li J.;
RT "Complete Genome Sequence of Paenibacillus mucilaginosus 3016, a Bacterium
RT Functional as Microbial Fertilizer.";
RL J. Bacteriol. 194:2777-2778(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
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DR EMBL; CP003235; AFC29526.1; -; Genomic_DNA.
DR RefSeq; WP_014369823.1; NC_016935.1.
DR AlphaFoldDB; H6NFB7; -.
DR STRING; 1116391.PM3016_2644; -.
DR KEGG; pmq:PM3016_2644; -.
DR HOGENOM; CLU_005015_3_2_9; -.
DR Proteomes; UP000007523; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 3.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 3.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:AFC29526.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000007523};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 201..303
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 671..760
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 763..845
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 864 AA; 98428 MW; AFF3775CAE5D683C CRC64;
MTITKPAYMT LSGWEFKACK DEHWLPAAVP GCVHTDLLRN GKIEDPFYGT NEKRLQWIDK
EDWEYRTSFA ASPEQLSRAN VELVFDGLDT YADVTLNGQP ILSADNMFRS WRADVKELLQ
EENTLRVRFR SPIQEDLPKL RALGYALPAA NDQSEAGGLG DEKVSVFARK APYHYGWDWG
PRFVTSGIWK DVRLESWSGI RIRDFHIRQD QVTAEAARLT ALVEVETDEA WSGELRVSAE
GRQWSRDVRL EAGSHTVQLA AELESPRLWW SRGLGAQELT DFRAELVSGG DVHAQETVTT
GLRSAKLVRQ KDAHGTSFYI ELNGVPVFCK GANHIPNDSF VTEVTYERYR HEVASAAEAN
MNMLRVWGGG IYEHDDFYRL CDEYGILVWQ DFMFACSMYP GDEAFLASVR SEAEENVKRL
RRHPSVVLWC GNNEMDTAWS HYDENAGWGW KKKYSPELRE KIWRDYEAIF HEILPETIRA
LSPETDYWPS SPMREVTGDA NQHATSSSPD GDIHYWGVWH NVEPFSNYKV HIGRFMSEYG
FQSFPEEKTV ETYAAEEDMA LVSEVMLSHQ KNGRGNMLIK EYMDQYLSEP KDFRSFLYMS
QVLQGEAMKT AIEAHRRRMG FTMGSLYWQM NDCWPVASWA GMDYLGRWKA LQYYAKRSFR
DVMLSLDEND GKVEIHGVSD LQVPLTGTLE LRLLDFQGNV LRQWPASVTL AANAAGHLKS
LAREEVLEGA DPASVVLTAR FEGSEGTTDL KEHYFLPVKD LRLEEPGVRI TRRTEGEQVL
FTLEADRLAK QVWLSSEAEG HFTDNFFDLL PGSPVTVAFR KRSDGGEPFA AGDPGSMQVR
SMYDFIKSSA GAQEADRQAE EQAL
//
