ID   H6U1M1_KLEPN            Unreviewed;       400 AA.
AC   H6U1M1;
DT   18-APR-2012, integrated into UniProtKB/TrEMBL.
DT   18-APR-2012, sequence version 1.
DT   24-JAN-2024, entry version 57.
DE   SubName: Full=Type II restriction enzyme {ECO:0000313|EMBL:AFB82800.1};
OS   Klebsiella pneumoniae.
OG   Plasmid pNDM-MAR {ECO:0000313|EMBL:AFB82800.1}.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573 {ECO:0000313|EMBL:AFB82800.1};
RN   [1] {ECO:0000313|EMBL:AFB82800.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=TCKpnC {ECO:0000313|EMBL:AFB82800.1};
RC   PLASMID=pNDM-MAR {ECO:0000313|EMBL:AFB82800.1};
RX   PubMed=22511638; DOI=10.1093/jac/dks114;
RA   Villa L., Poirel L., Nordmann P., Carta C., Carattoli A.;
RT   "Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15
RT   and qnrB1 genes.";
RL   J. Antimicrob. Chemother. 67:1645-1650(2012).
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; JN420336; AFB82800.1; -; Genomic_DNA.
DR   RefSeq; WP_014386493.1; NZ_UJWI01000033.1.
DR   RefSeq; YP_005352196.1; NC_016980.1.
DR   AlphaFoldDB; H6U1M1; -.
DR   REBASE; 47989; KpnTCKORFBP.
DR   REBASE; 94612; KpnPMK1ORF125P.
DR   KEGG; kpx:PMK1_ndm00124; -.
DR   PATRIC; fig|573.1358.peg.5679; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR   Gene3D; 3.40.91.80; -; 1.
DR   Gene3D; 2.40.330.10; DNA-binding pseudobarrel domain; 1.
DR   InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR   InterPro; IPR038365; EcoRII_C_sf.
DR   InterPro; IPR023372; Rest_endonuc_II_EcoRII_N.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR015109; Restrct_endonuc_II_EcoRII_C.
DR   Pfam; PF09019; EcoRII-C; 1.
DR   Pfam; PF09217; EcoRII-N; 1.
DR   SUPFAM; SSF101936; DNA-binding pseudobarrel domain; 1.
DR   SUPFAM; SSF52980; Restriction endonuclease-like; 1.
PE   4: Predicted;
KW   Plasmid {ECO:0000313|EMBL:AFB82800.1}.
FT   DOMAIN          20..162
FT                   /note="Restriction endonuclease type II EcoRII N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF09217"
FT   DOMAIN          228..392
FT                   /note="Restriction endonuclease type II EcoRII C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF09019"
SQ   SEQUENCE   400 AA;  44999 MW;  743CE92E38F8CEB8 CRC64;
     MCIHLNLMIF TKIIRGFISV KRLSANDTGA TRSHQSGLYM PGAVIDELFP SLRDTQLRNP
     EALFSVHVSS HPDCPDLDDV RAIYYNNKFF GGTRDEKRLT GFGRQNPLQN PENTGALALL
     AFDHVPGTSS SYCDVWVCKD LTEEDILEPI IGEVIPGATI FGPGDKIIGG FVTETSPGRT
     KYKLPPEWTH HFPSGREIIS FAACHYDRTT DDPDSQLTRR RKIEFEIFLA VEELHVLGQI
     AEGFATVNDF ITLANSVSNR RKSRAGKSLE LHLESLFKEH GATSFETQAI TEGKKKPDFI
     FPSGAAYHDP DYPAERLRML GVKTTCKDRW RQVLNEADRI DTVHLFTVQQ GVSVAQFREM
     QSEGIRLVVP VGLHKAFPEE IRGELMSLSA FIDEIKKLYW
//