ID H7FZT8_9LACO Unreviewed; 529 AA.
AC H7FZT8;
DT 16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT 16-MAY-2012, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=SMXD51_05853 {ECO:0000313|EMBL:EIA32330.1};
OS Ligilactobacillus salivarius SMXD51.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Ligilactobacillus.
OX NCBI_TaxID=1108963 {ECO:0000313|EMBL:EIA32330.1, ECO:0000313|Proteomes:UP000003657};
RN [1] {ECO:0000313|EMBL:EIA32330.1, ECO:0000313|Proteomes:UP000003657}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SMXD51 {ECO:0000313|EMBL:EIA32330.1,
RC ECO:0000313|Proteomes:UP000003657};
RX PubMed=22582370; DOI=10.1128/JB.00344-12;
RA Kergourlay G., Messaoudi S., Dousset X., Prevost H.;
RT "Genome Sequence of Lactobacillus salivarius SMXD51, a Potential Probiotic
RT Strain Isolated from Chicken Cecum, Showing Anti-Campylobacter Activity.";
RL J. Bacteriol. 194:3008-3009(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIA32330.1}.
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DR EMBL; AICL01000004; EIA32330.1; -; Genomic_DNA.
DR RefSeq; WP_003710193.1; NZ_AICL01000004.1.
DR AlphaFoldDB; H7FZT8; -.
DR REBASE; 48763; M.Lsa51ORF5853P.
DR PATRIC; fig|1108963.3.peg.646; -.
DR HOGENOM; CLU_013049_0_2_9; -.
DR Proteomes; UP000003657; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 8..156
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 167..471
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 478..509
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 529 AA; 60847 MW; 36512C3CBB458015 CRC64;
MAQEEVTLER SLDNAANVLR SKMDANEYKN YTLGTIFYKY LSDSMLYYVA ELLEEKNISL
EEAQKLYEEN QDDQYLIEEL DIKFNYVIEA KNTYTNILKS INNHTFQVSQ LGDAFNSIES
QGKEFEGLFD DYDLYSKRLG NTAQKQSDTI SEVLSAIGKL EIVKTPEDTL GNAYEYLIKQ
FASESGKKAG EFYTPQKVSR LLARLTLVDK DYTDGMTVYD PTMGSGSLLL NFRKYVEHSE
RITYFGQEIN TSTYNLARMN MILHHVDVVN QKLRNNDTLD EDWPVEEITN FDAVVMNPPY
SQKWSANAGF KDDPRFSAYG VLPPKSKADY AFLLHGYYHL KHSGVMAIVL PHGILFRGAA
EGKIRKKLLE NGAIDAVIGL PANLFYNTSI PTTIVVLKKD KQDRDVLFID ASKDFKKVKT
QNELRDEDVE KILTTYKERK DIDKYAHLAS FDEIKENEFN LNIPRYVDTF EPEPEINLDE
VSKELRETNE KIKENETELI SMLKDLTSED EKIMKGLNDF ISVLEEDVK
//
