ID H8H131_DEIGI Unreviewed; 666 AA.
AC H8H131;
DT 16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT 16-MAY-2012, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN OrderedLocusNames=DGo_PA0164 {ECO:0000313|EMBL:AFD27050.1};
OS Deinococcus gobiensis (strain DSM 21396 / JCM 16679 / CGMCC 1.7299 / I-0).
OG Plasmid P1 {ECO:0000313|EMBL:AFD27050.1,
OG ECO:0000313|Proteomes:UP000007575}.
OC Bacteria; Deinococcota; Deinococci; Deinococcales; Deinococcaceae;
OC Deinococcus.
OX NCBI_TaxID=745776 {ECO:0000313|EMBL:AFD27050.1, ECO:0000313|Proteomes:UP000007575};
RN [1] {ECO:0000313|EMBL:AFD27050.1, ECO:0000313|Proteomes:UP000007575}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 21396 / JCM 16679 / CGMCC 1.7299 / I-0
RC {ECO:0000313|Proteomes:UP000007575};
RC PLASMID=Plasmid P1 {ECO:0000313|Proteomes:UP000007575};
RX PubMed=22470573; DOI=10.1371/journal.pone.0034458;
RA Yuan M., Chen M., Zhang W., Lu W., Wang J., Yang M., Zhao P., Tang R.,
RA Li X., Hao Y., Zhou Z., Zhan Y., Yu H., Teng C., Yan Y., Ping S., Wang Y.,
RA Lin M.;
RT "Genome sequence and transcriptome analysis of the radioresistant bacterium
RT Deinococcus gobiensis: insights into the extreme environmental
RT adaptations.";
RL PLoS ONE 7:E34458-E34458(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR EMBL; CP002192; AFD27050.1; -; Genomic_DNA.
DR RefSeq; WP_014695568.1; NC_017805.1.
DR AlphaFoldDB; H8H131; -.
DR KEGG; dgo:DGo_PA0164; -.
DR PATRIC; fig|745776.4.peg.3200; -.
DR HOGENOM; CLU_012430_1_0_0; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000007575; Plasmid P1.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Plasmid {ECO:0000313|EMBL:AFD27050.1}.
FT DOMAIN 20..407
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 419..617
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 154
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 336
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 115
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 153
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 344
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 666 AA; 73208 MW; 2155BF0A31747F89 CRC64;
MSSLPPSDSL PFLSLGVCDY PEHVPQPLWA GYARDQKALG LEFVRLAEFA WSRMEPRPGE
YDWAWLDEAV ETSAAAGLKV VLCTPTAAPP AWLVEAHPEI LPVGRDGQVK SFGSRRHYDF
SSAVYREHSR RITRAVAERY GRHPAVVGWQ TDNEFGWGDT AQSFSPAVHA AFHAWLERRY
GTVDALNAAW GNVFWSMDYS GWAQVPLPHH AVSEINPAHA LDFLRFSSGE VAAFQAEQVA
LLREHSPGRF VTHNYMGFFS AYDHYEVSRG LDFASWDSYP TGTLQAIHEW KLLAPHFALD
YARTGHPDVT AFNHDLYRGM VTPGAGQATP SFWVMEQQCG QVNWASANPL PAPGAVALWT
EQAWAHGADA VSYFRWRAAT VGQEVLHSGL LRHSGRPDQG HREVAALRPQ ALPLAPTRAR
VALLHDYESL WLYNLQPHSE GLNYWAQTFT YYRALRALGV DVDILHPDRD LSGYAVVVAP
ALTLMTPERA AHLTASAGHS RLVFGPRTAF RTAAGGTPET GQFGDLAGLI GAELSRYDSL
YAGMTQDVEG EGGARHSAHA WAESYDLAGA EALWHYRGGP LDGEAAVVRH GAVTTVGAHS
ESLVRGVLRG ALREAGVETA ELPEGVRLTR RGPHTLLQNW TGESVHWQGR TLAPYSSALL
PQEVMA
//
