ID H8ZHZ8_9REOV Unreviewed; 313 AA.
AC H8ZHZ8;
DT 16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT 16-MAY-2012, sequence version 1.
DT 03-MAY-2023, entry version 24.
DE RecName: Full=Non-structural protein 3 {ECO:0000256|HAMAP-Rule:MF_04094};
DE Short=NSP3 {ECO:0000256|HAMAP-Rule:MF_04094};
DE AltName: Full=NCVP4 {ECO:0000256|HAMAP-Rule:MF_04094};
DE AltName: Full=Non-structural RNA-binding protein 34 {ECO:0000256|HAMAP-Rule:MF_04094};
DE Short=NS34 {ECO:0000256|HAMAP-Rule:MF_04094};
GN Name=NSP3 {ECO:0000313|EMBL:AFC90177.1};
OS Human rotavirus A.
OC Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC Reovirales; Sedoreoviridae; Rotavirus; Rotavirus A.
OX NCBI_TaxID=10941 {ECO:0000313|EMBL:AFC90177.1, ECO:0000313|Proteomes:UP000122236};
RN [1] {ECO:0000313|Proteomes:UP000122236}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8874505; DOI=10.1006/viro.1996.0551;
RA Kojima K., Taniguchi K., Urasawa T., Urasawa S.;
RT "Sequence analysis of normal and rearranged NSP5 genes from human rotavirus
RT strains isolated in nature: implications for the occurrence of the
RT rearrangement at the step of plus strand synthesis.";
RL Virology 224:446-452(1996).
RN [2] {ECO:0000313|Proteomes:UP000122236}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10568765;
RX DOI=10.1002/(SICI)1096-9071(200001)60:1<63::AID-JMV11>3.3.CO;2-W;
RA Okada J., Urasawa T., Kobayashi N., Taniguchi K., Hasegawa A., Mise K.,
RA Urasawa S.;
RT "New P serotype of group A human rotavirus closely related to that of a
RT porcine rotavirus.";
RL J. Med. Virol. 60:63-69(2000).
RN [3] {ECO:0000313|EMBL:AFC90177.1, ECO:0000313|Proteomes:UP000122236}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mc345 {ECO:0000313|EMBL:AFC90177.1};
RX PubMed=22226701; DOI=10.1016/j.meegid.2011.12.012;
RA Ghosh S., Urushibara N., Taniguchi K., Kobayashi N.;
RT "Whole genomic analysis reveals the porcine origin of human G9P[19]
RT rotavirus strains Mc323 and Mc345.";
RL Infect. Genet. Evol. 12:471-477(2012).
CC -!- FUNCTION: Plays an important role in stimulating the translation of
CC viral mRNAs. These mRNAs are capped but not polyadenylated, instead
CC terminating in a conserved sequence 'GACC' at the 3' that is recognized
CC by NSP3, which competes with host PABPC1 for EIF4G1 binding. The
CC interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1
CC interaction, thereby facilitating the initiation of capped mRNA
CC translation. {ECO:0000256|HAMAP-Rule:MF_04094}.
CC -!- SUBUNIT: Homodimer. Interacts (via the coiled-coil region) with host
CC ZC3H7B (via LD motif). Interacts with host EIF4G1. {ECO:0000256|HAMAP-
CC Rule:MF_04094}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000256|HAMAP-Rule:MF_04094}.
CC -!- SIMILARITY: Belongs to the rotavirus A NSP3 protein family.
CC {ECO:0000256|HAMAP-Rule:MF_04094}.
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DR EMBL; JN104625; AFC90177.1; -; Genomic_RNA.
DR Proteomes; UP000122236; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR CDD; cd20714; NSP3_rotavirus; 1.
DR Gene3D; 3.30.70.1610; -; 1.
DR Gene3D; 1.20.5.970; Nonstructural RNA-binding protein; 1.
DR Gene3D; 6.10.280.20; Rotavirus non-structural protein NSP3, N-terminal domain; 1.
DR HAMAP; MF_04094; ROTA_A_NSP3; 1.
DR HAMAP; MF_04090; ROTA_NSP3; 1.
DR InterPro; IPR042519; NSP3_N_rotavirus.
DR InterPro; IPR036082; NSP3_sf.
DR InterPro; IPR002873; Rotavirus_NSP3.
DR Pfam; PF01665; Rota_NSP3; 1.
DR SUPFAM; SSF69903; NSP3 homodimer; 1.
DR SUPFAM; SSF58030; Rotavirus nonstructural proteins; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|HAMAP-
KW Rule:MF_04094}; Host cytoplasm {ECO:0000256|HAMAP-Rule:MF_04094};
KW Host-virus interaction {ECO:0000256|HAMAP-Rule:MF_04094};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_04094};
KW Translation regulation {ECO:0000256|ARBA:ARBA00022845, ECO:0000256|HAMAP-
KW Rule:MF_04094}.
FT REGION 1..149
FT /note="RNA-binding"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
FT REGION 150..206
FT /note="Dimerization"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
FT REGION 170..234
FT /note="Interaction with host ZC3H7B"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
FT REGION 208..313
FT /note="Interaction with host EIF4G1"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
FT COILED 166..237
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
SQ SEQUENCE 313 AA; 36110 MW; D8EE354C42A7F7CF CRC64;
MLKMESTQQM VGSIINTSFE AAVVAATSTL ELMGIQYDYN EVFTRVKSKF DYVMDDSGVK
NNLLGKAMTI DQALNGKFGS VMRNRNWMTD SKTVAKLDED VNKLRMMLSS KGIDQKMRVL
NACFSVKRIP GKSSSVIKCT RLMKDKIERG EVEVDDSFVD EKMEVDTIDW KSRYDQLEKR
FESLKQRVNE KYNSWVQKAK KVNENMYSLQ NVISQQQNQI ADLQQYCNKL EADLQSKIGS
LVSSVEWYLR SMELSDDIKT DIEQQLNSID AINPINAIDD LESLIRNLIQ DYDRTFLMLK
GLVRQCNYEC AYE
//
