ID I0KW29_9ACTN Unreviewed; 818 AA.
AC I0KW29;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=galactosylceramidase {ECO:0000256|ARBA:ARBA00012657};
DE EC=3.2.1.46 {ECO:0000256|ARBA:ARBA00012657};
DE AltName: Full=Galactosylceramidase {ECO:0000256|ARBA:ARBA00033098};
GN ORFNames=MILUP08_40687 {ECO:0000313|EMBL:CCH15776.1};
OS Micromonospora lupini str. Lupac 08.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=1150864 {ECO:0000313|EMBL:CCH15776.1, ECO:0000313|Proteomes:UP000003448};
RN [1] {ECO:0000313|Proteomes:UP000003448}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Lupac 08 {ECO:0000313|Proteomes:UP000003448};
RX PubMed=22815450; DOI=10.1128/jb.00628-12;
RA Alonso-Vega P., Normand P., Bacigalupe R., Pujic P., Lajus A., Vallenet D.,
RA Carro L., Coll P., Trujillo M.E.;
RT "Genome Sequence of Micromonospora lupini Lupac 08, Isolated from Root
RT Nodules of Lupinus angustifolius.";
RL J. Bacteriol. 194:4135-4135(2012).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 59 family.
CC {ECO:0000256|ARBA:ARBA00005637}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCH15776.1}.
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DR EMBL; CAIE01000010; CCH15776.1; -; Genomic_DNA.
DR AlphaFoldDB; I0KW29; -.
DR STRING; 1150864.MILUP08_40687; -.
DR eggNOG; COG5520; Bacteria.
DR OrthoDB; 9806701at2; -.
DR Proteomes; UP000003448; Unassembled WGS sequence.
DR GO; GO:0004336; F:galactosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006683; P:galactosylceramide catabolic process; IEA:InterPro.
DR CDD; cd00161; RICIN; 1.
DR Gene3D; 2.80.10.50; -; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR049162; GH59_C.
DR InterPro; IPR049161; GH59_cat.
DR InterPro; IPR001286; Glyco_hydro_59.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR PANTHER; PTHR15172; GALACTOCEREBROSIDASE; 1.
DR PANTHER; PTHR15172:SF1; GALACTOCEREBROSIDASE; 1.
DR Pfam; PF02057; Glyco_hydro_59; 1.
DR Pfam; PF21708; Glyco_hydro_59_C; 1.
DR Pfam; PF00652; Ricin_B_lectin; 1.
DR PRINTS; PR00850; GLHYDRLASE59.
DR SMART; SM00458; RICIN; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:CCH15776.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:CCH15776.1};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022963};
KW Reference proteome {ECO:0000313|Proteomes:UP000003448};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Sphingolipid metabolism {ECO:0000256|ARBA:ARBA00022919}.
FT DOMAIN 694..818
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..20
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 818 AA; 86202 MW; 5E5B1E0EA21317D0 CRC64;
MFDRESHRRG AGEGGPRRRS WRSTWRPRRL TASLAGAVVL AAAVLTGFAS PAQAATAITI
NGGSGGRALD GIGAVSGGGG NSRLLIDYPE PQRSDILDYL FKPGRGAAMQ LLKTEIGGDT
NSTSGAEPSH EHTRGAINCN RGYEWWLMEQ ARSRNANIAL AGLAWGAPGW IGNRNFWSQD
SIDYLVAWLD CAATHGLTIN YLGGWNEKGY NAGWYKNLRS ALNSHGYSNV KIVASDDFGW
GAADESLRDP AFANAVQVFG SHYVCGYRSA QTSCPSSGNA VSTGKQLWAS ENGSDDYNAG
AQALARGINR GYIDGKMTGY INWPAIAAIT PNIPWATTGV AVAQQPWSGA YSIGKNTWVM
AQTTQFTAPG WRYLDSSSGY LGGNRNNGSY VSLKSTNNSD YSTIIETMDA GSAQTLDFTV
TGGLSTGAVH VWSTNVRSNN TADHFVRGSD ITPSGGRFSL TVQPGYVYSI TTTTGQGKST
ATSPAAGALK LPYNDTFDSY AVGREAKYLM DQQGSFEIAG CAAGRAGLCV RQMSEQTPIF
WTSGQAEPFT LLGDLSWRNY TVSSDVLLEK SGYAQLIGRA NTYNHQGPQN LNGYYFRVTD
GGAWSIRSNN TSGNWRTLAS GNTSALGTGR WHTLSLTLNG STLSASIDGT TVGTVSDSTW
VAGQIGYGTG QGVTAQFDNL TITPVGNSGE PGATGTIRGA GSNRCLDVNG ASQADGSVVQ
IWDCNSGANQ QWTLTASNQL TVYGNKCLDV AGTATGSRAR IMTCNGGSGQ QWRVNSDGTI
SGVGSGLCLD VNGAGTANGT AVQTWTCTGG SNQQWIRG
//
