ID I1D1R9_9PSEU Unreviewed; 919 AA.
AC I1D1R9;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE SubName: Full=CRISPR-associated helicase Cas3/CRISPR-associated endonuclease Cas3-HD {ECO:0000313|EMBL:EIE98893.1};
GN ORFNames=SacglDRAFT_01986 {ECO:0000313|EMBL:EIE98893.1};
OS Saccharomonospora glauca K62.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharomonospora.
OX NCBI_TaxID=928724 {ECO:0000313|EMBL:EIE98893.1, ECO:0000313|Proteomes:UP000005087};
RN [1] {ECO:0000313|EMBL:EIE98893.1, ECO:0000313|Proteomes:UP000005087}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K62 {ECO:0000313|EMBL:EIE98893.1,
RC ECO:0000313|Proteomes:UP000005087};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Land M.L., Hauser L., Brambilla E., Klenk H.-P., Woyke T.J.;
RL Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000005087}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K62 {ECO:0000313|Proteomes:UP000005087};
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Mikhailova N., Held B., Detter J.C., Han C., Tapia R., Land M.,
RA Hauser L., Kyrpides N., Ivanova N., Pagani I., Brambilla E.-M.,
RA Klenk H.-P., Woyke T.;
RT "Noncontiguous Finished sequence of chromosome of Saccharomonospora glauca
RT K62.";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
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DR EMBL; CM001484; EIE98893.1; -; Genomic_DNA.
DR RefSeq; WP_005464016.1; NZ_CM001484.1.
DR AlphaFoldDB; I1D1R9; -.
DR STRING; 928724.SacglDRAFT_01986; -.
DR eggNOG; COG1203; Bacteria.
DR HOGENOM; CLU_013924_1_0_11; -.
DR OrthoDB; 9810236at2; -.
DR Proteomes; UP000005087; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR041372; Cas3_C.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR PANTHER; PTHR47963:SF9; CRISPR-ASSOCIATED ENDONUCLEASE_HELICASE CAS3; 1.
DR PANTHER; PTHR47963; DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL; 1.
DR Pfam; PF18395; Cas3_C; 1.
DR Pfam; PF18019; Cas3_HD; 1.
DR Pfam; PF00270; DEAD; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Endonuclease {ECO:0000313|EMBL:EIE98893.1};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000005087}.
FT DOMAIN 15..204
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 278..483
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 919 AA; 101509 MW; ABD8F8EF37367307 CRC64;
MLLVDLRFWG KERGLEGARY PLVCHGLDAA AAVRWLWKRY LSARVRAGLA EAVGLTEEQT
CRVLEFWAAA HDVGKLTPGF QEQVGVPEGY LPDSEGRRCR HEEASHIWLP SALTAVEHTT
NSRNARLIAQ MLGGHHGVFR RRDSADFRPD MFVHLGLGDS SWNLQRHAHL QAWRALLAPP
TLPQRLSRHA AAVATGVVIL ADWLVSQIDY VRSRLPFLPE QGDLPALSKF LHESREATES
VVRQAGLSRL RLRGGTFEEE FGFDEPNELQ SSIAAELPGL LGNPGLLMIA APTGFGKTEA
ALHAARLLSD TAGTSGMFFA LPTMATSDQM FDRIARYVVR RAETGVAQSL LHGMAWLKPL
RETLEQIHAE EELSSDDETQ VHGLEWLQGL KRAMLAPVGV GTIDQALLAV LPVRHNALRL
FSLLGKTVVI DEVHAFSPYM RRLLCTLLSW LGEWNVPVVL LSATLPRNIA AELAAAYRGQ
NTGTPSDVPV PYPGWTYIER ESEIKTRPVD FPRSHRRTLS VQLRPVAVAR ESGPNRLPVL
REVLAPIVAD EGGGCALVLC TTVAEAQQTY RALRDWLGET DVDLRLLHAR YPMHQRETLT
TELMRAFSKP QHGDGGASQA GNRPAKAVVV ATQVVEQSLD LDFDLVVSDL APIELLLQRA
GRLQRHSGWD PHRPAWADVS RGGQRRFIVL TAPDGDLHRL PRSWKFIYPP ISLIRAHRLL
AERTALGVRI PDDVQELVDR GNPGQFPDLD DPSVSGFTEE EIRRSAESLV ETGTADSASI
PVPKALTNLA DLSKGPLEEE QVTTRFNADS HRALPVFRTH DGALRLGGAH GEPLPVPREG
KLSKDELKSI MKHTVPVPGS VVRGKDERHQ LPEPWREITV LRDLVVLPHH IDEDGTVHPA
SVGGRKLLLD DVLGLLAVE
//
