ID I1J6T1_SOYBN Unreviewed; 388 AA.
AC I1J6T1;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=chalcone synthase {ECO:0000256|ARBA:ARBA00012975};
DE EC=2.3.1.74 {ECO:0000256|ARBA:ARBA00012975};
GN Name=100785554 {ECO:0000313|EnsemblPlants:KRH75544};
GN ORFNames=GLYMA_01G091400 {ECO:0000313|EMBL:KRH75544.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EnsemblPlants:KRH75544};
RN [1] {ECO:0000313|EMBL:KRH75544.1, ECO:0000313|EnsemblPlants:KRH75544}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH75544};
RC TISSUE=Callus {ECO:0000313|EMBL:KRH75544.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRH75544}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH75544};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRH75544.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRH75544.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin. {ECO:0000256|ARBA:ARBA00002969}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004966}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|RuleBase:RU003633}.
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DR EMBL; CM000834; KRH75544.1; -; Genomic_DNA.
DR RefSeq; XP_003516867.1; XM_003516819.3.
DR AlphaFoldDB; I1J6T1; -.
DR SMR; I1J6T1; -.
DR STRING; 3847.I1J6T1; -.
DR PaxDb; 3847-GLYMA01G22880-1; -.
DR EnsemblPlants; KRH75544; KRH75544; GLYMA_01G091400.
DR Gramene; KRH75544; KRH75544; GLYMA_01G091400.
DR eggNOG; ENOG502QRSY; Eukaryota.
DR HOGENOM; CLU_034992_2_0_1; -.
DR InParanoid; I1J6T1; -.
DR OMA; GMCAYMA; -.
DR OrthoDB; 883077at2759; -.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000008827; Chromosome 1.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0030639; P:polyketide biosynthetic process; IBA:GO_Central.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003633};
KW Flavonoid biosynthesis {ECO:0000256|ARBA:ARBA00023241};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003633}.
FT DOMAIN 5..228
FT /note="Chalcone/stilbene synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00195"
FT DOMAIN 237..387
FT /note="Chalcone/stilbene synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02797"
FT ACT_SITE 164
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000451-1"
SQ SEQUENCE 388 AA; 42475 MW; 45C7AFE0184C794C CRC64;
MVSVEEIRKA QRAEGPATVM AIGTATPPNC VDQSTYPDYY FRITNSDHMT ELKEKFKRMC
DKSMIKKRYM YLNEEILKEN PSVCAYMAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP
KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPSVKRYMMY QQGCFAGGTV LRLAKDLAEN
NTGARVLVVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AVILGSDPLP AEKPLFELVW
TAQTILPDSE GAIDGHLREV GLTFHLLKDV PGLISKNIQK ALVEAFQPLG IDDYNSIFWI
AHPGGPAILD QVEAKLGLKP EKMEATRHVL SEYGNMSSAC VLFILDQMRK KSIENGLGTT
GEGLEWGVLF GFGPGLTVET VVLRSVTV
//
