ID   I1QF45_ORYGL            Unreviewed;       589 AA.
AC   I1QF45;
DT   13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT   13-JUN-2012, sequence version 1.
DT   27-MAR-2024, entry version 43.
DE   RecName: Full=L-gulonolactone oxidase {ECO:0000256|ARBA:ARBA00013121};
DE            EC=1.1.3.8 {ECO:0000256|ARBA:ARBA00013121};
OS   Oryza glaberrima (African rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX   NCBI_TaxID=4538 {ECO:0000313|EnsemblPlants:ORGLA08G0004600.1, ECO:0000313|Proteomes:UP000007306};
RN   [1] {ECO:0000313|Proteomes:UP000007306}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IRGC 96717 {ECO:0000313|Proteomes:UP000007306};
RA   Wing R.A., Yu Y., Rounsley S., Reddy-Marri P., Goicoechea J.L.,
RA   Sisneros N., Lee S., Song X., Angelova A., Kudrna D.P., de Baynast K.,
RA   Zuccolo A.;
RT   "The complete genome of Oryza glaberrima.";
RL   Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblPlants:ORGLA08G0004600.1}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (JUN-2015) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-gulono-1,4-lactone + O2 = H(+) + H2O2 + L-ascorbate;
CC         Xref=Rhea:RHEA:32363, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:17587, ChEBI:CHEBI:38290; EC=1.1.3.8;
CC         Evidence={ECO:0000256|ARBA:ARBA00001630};
CC   -!- PATHWAY: Cofactor biosynthesis; L-ascorbate biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00005147}.
CC   -!- SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase
CC       family. {ECO:0000256|ARBA:ARBA00005466}.
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DR   AlphaFoldDB; I1QF45; -.
DR   STRING; 4538.I1QF45; -.
DR   EnsemblPlants; ORGLA08G0004600.1; ORGLA08G0004600.1; ORGLA08G0004600.
DR   Gramene; ORGLA08G0004600.1; ORGLA08G0004600.1; ORGLA08G0004600.
DR   eggNOG; KOG4730; Eukaryota.
DR   HOGENOM; CLU_019762_2_0_1; -.
DR   OMA; VSWLPRQ; -.
DR   UniPathway; UPA00132; -.
DR   Proteomes; UP000007306; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0003885; F:D-arabinono-1,4-lactone oxidase activity; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0050105; F:L-gulonolactone oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019853; P:L-ascorbic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.465.10; -; 1.
DR   Gene3D; 3.30.70.2520; -; 1.
DR   InterPro; IPR007173; ALO_C.
DR   InterPro; IPR016166; FAD-bd_PCMH.
DR   InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR   InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR   InterPro; IPR010030; GULO_Plant.
DR   InterPro; IPR006094; Oxid_FAD_bind_N.
DR   NCBIfam; TIGR01677; pln_FAD_oxido; 1.
DR   PANTHER; PTHR13878; GULONOLACTONE OXIDASE; 1.
DR   PANTHER; PTHR13878:SF90; L-GULONOLACTONE OXIDASE; 1.
DR   Pfam; PF04030; ALO; 1.
DR   Pfam; PF01565; FAD_binding_4; 1.
DR   SUPFAM; SSF56176; FAD-binding/transporter-associated domain-like; 1.
DR   PROSITE; PS51387; FAD_PCMH; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           21..589
FT                   /note="L-gulonolactone oxidase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5003650079"
FT   DOMAIN          49..229
FT                   /note="FAD-binding PCMH-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51387"
SQ   SEQUENCE   589 AA;  63649 MW;  48B4C1DEA966DBA0 CRC64;
     MRTMLPLLVI LVVGLRLAGA SPPPQPVACT KGTTDCTVTN VYGSFPDRTI CRAADASFPR
     TEAELVAAVA AAAAAGRKAK AATRHSHSFP KLACPGGRDG TIISTRFLNR TVAFDAAARR
     ITVESGVVLR DLIRAAAAAG LALPHSPYWY GLTVGGLLAT GAHGSSLWGK GSAVHEYVVG
     LRIVTPAPAS QGFAVVRELV AGDPDLDAAK VSLGVLGVIS QVTFELQPQF KRSVRFVTRD
     DSDFAEKVAV WGGAHEFGDM AWLPRQGKVI YREDDRVDVA TPGNGLNDYL GFRAQPTLGL
     ITARAAEERL ERNGTDIARC LAARLPPSLF ELQAYGFTND GVFFTGWPVV GFQHRIQASG
     TCISSPEDGL LSSCTWDPRI RGPFLYNSGF SIALPRAAAF VADMMRLRDL NPRAFCDIDA
     KLGILMRYVK ASSAYLGKPE DCVDFDVTYY RSYDDGEPRP HSDVFDELEQ MALRKYGAVP
     HWGKNRNFAF DGAAAKYPNS GEFIKVKERY DPDGIFSSEW SDQVLGISGS PNIVDKRCAI
     EGLCVCSDDS HCAPELGYFC RPGKLFKEAR VCSKDKSAAA GDDDLLDEL
//