ID I3J254_ORENI Unreviewed; 277 AA.
AC I3J254;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=GCS light chain {ECO:0000256|ARBA:ARBA00031732};
DE AltName: Full=Gamma-ECS regulatory subunit {ECO:0000256|ARBA:ARBA00030406};
DE AltName: Full=Gamma-glutamylcysteine synthetase regulatory subunit {ECO:0000256|ARBA:ARBA00032926};
DE AltName: Full=Glutamate--cysteine ligase modifier subunit {ECO:0000256|ARBA:ARBA00031154};
GN Name=gclm {ECO:0000313|Ensembl:ENSONIP00000002944.2};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000002944.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000002944.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC L-cysteine and L-glutamate: step 1/2. {ECO:0000256|ARBA:ARBA00005006}.
CC -!- SUBUNIT: Heterodimer of a catalytic heavy chain and a regulatory light
CC chain. {ECO:0000256|ARBA:ARBA00011532}.
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family.
CC Glutamate--cysteine ligase light chain subfamily.
CC {ECO:0000256|ARBA:ARBA00008612}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR RefSeq; XP_003439702.1; XM_003439654.4.
DR AlphaFoldDB; I3J254; -.
DR STRING; 8128.ENSONIP00000002944; -.
DR Ensembl; ENSONIT00000002945.2; ENSONIP00000002944.2; ENSONIG00000002354.2.
DR GeneID; 100708953; -.
DR KEGG; onl:100708953; -.
DR CTD; 2730; -.
DR eggNOG; KOG3023; Eukaryota.
DR GeneTree; ENSGT00510000047658; -.
DR HOGENOM; CLU_055657_1_0_1; -.
DR InParanoid; I3J254; -.
DR OMA; AHEWIPL; -.
DR OrthoDB; 1706825at2759; -.
DR TreeFam; TF105986; -.
DR UniPathway; UPA00142; UER00209.
DR Proteomes; UP000005207; Linkage group LG23.
DR GO; GO:0035226; F:glutamate-cysteine ligase catalytic subunit binding; IEA:InterPro.
DR GO; GO:0006750; P:glutathione biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR032963; Gclm.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR13295; GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNIT; 1.
DR PANTHER; PTHR13295:SF4; GLUTAMATE--CYSTEINE LIGASE REGULATORY SUBUNIT; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW Glutathione biosynthesis {ECO:0000256|ARBA:ARBA00022684};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207}.
FT DOMAIN 86..216
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
SQ SEQUENCE 277 AA; 30651 MW; 024DED0D0DC92FFD CRC64;
MDSQNTTNTN TEAKMLLSHA KTFRLHTGNL VNRSRLKKKC PGSPSEELRD CIQASLSDWI
STTKLSSKDL TDTLEFSIPE ATDAITPEER EELKVSVKLF LRESGQSSIK DAVEMACQTL
AVSQLDSVII APPGPSDGDS QSLAHLQPVW EELEALVRSK QIAAIGTSDL DKDLLEQLYK
WAQIKPSSNQ VNLASCCVMP PDLTAFAKEF DIQLLTHNDT KELISADTFQ EAVRAGAQDL
NIGDWRLEWV LRYNVIVKSR GIIKSKGYLV SAKKTST
//
