ID I3K269_ORENI Unreviewed; 4802 AA.
AC I3K269;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 75.
DE SubName: Full=Ryanodine receptor 3 {ECO:0000313|Ensembl:ENSONIP00000015214.2};
GN Name=RYR3 {ECO:0000313|Ensembl:ENSONIP00000015214.2};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000015214.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000015214.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Sarcoplasmic
CC reticulum membrane {ECO:0000256|ARBA:ARBA00004326}; Multi-pass membrane
CC protein {ECO:0000256|ARBA:ARBA00004326}.
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DR STRING; 8128.ENSONIP00000042600; -.
DR Ensembl; ENSONIT00000015227.2; ENSONIP00000015214.2; ENSONIG00000012087.2.
DR eggNOG; KOG2243; Eukaryota.
DR GeneTree; ENSGT00940000155507; -.
DR HOGENOM; CLU_000040_2_0_1; -.
DR TreeFam; TF315244; -.
DR Proteomes; UP000005207; Linkage group LG19.
DR GO; GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0005219; F:ryanodine-sensitive calcium-release channel activity; IEA:InterPro.
DR GO; GO:0006874; P:intracellular calcium ion homeostasis; IEA:InterPro.
DR CDD; cd12877; SPRY1_RyR; 1.
DR CDD; cd12878; SPRY2_RyR; 1.
DR CDD; cd12879; SPRY3_RyR; 1.
DR Gene3D; 1.10.287.70; -; 1.
DR Gene3D; 1.10.490.160; -; 2.
DR Gene3D; 2.60.120.920; -; 3.
DR Gene3D; 2.80.10.50; -; 2.
DR Gene3D; 6.20.350.10; -; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 1.25.10.30; IP3 receptor type 1 binding core, RIH domain; 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR014821; Ins145_P3_rcpt.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR036300; MIR_dom_sf.
DR InterPro; IPR016093; MIR_motif.
DR InterPro; IPR013662; RIH_assoc-dom.
DR InterPro; IPR000699; RIH_dom.
DR InterPro; IPR013333; Ryan_recept.
DR InterPro; IPR015925; Ryanodine_IP3_receptor.
DR InterPro; IPR003032; Ryanodine_rcpt.
DR InterPro; IPR009460; Ryanrecept_TM4-6.
DR InterPro; IPR048581; RYDR_Jsol.
DR InterPro; IPR035910; RyR/IP3R_RIH_dom_sf.
DR InterPro; IPR035761; SPRY1_RyR.
DR InterPro; IPR035764; SPRY2_RyR.
DR InterPro; IPR035762; SPRY3_RyR.
DR InterPro; IPR003877; SPRY_dom.
DR PANTHER; PTHR46399; B30.2/SPRY DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR46399:SF9; RYANODINE RECEPTOR 3; 1.
DR Pfam; PF08709; Ins145_P3_rec; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR Pfam; PF02815; MIR; 1.
DR Pfam; PF08454; RIH_assoc; 1.
DR Pfam; PF06459; RR_TM4-6; 1.
DR Pfam; PF01365; RYDR_ITPR; 2.
DR Pfam; PF21119; RYDR_Jsol; 1.
DR Pfam; PF02026; RyR; 4.
DR Pfam; PF00622; SPRY; 3.
DR PRINTS; PR00795; RYANODINER.
DR SMART; SM00472; MIR; 4.
DR SMART; SM00449; SPRY; 3.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 3.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF100909; IP3 receptor type 1 binding core, domain 2; 2.
DR SUPFAM; SSF82109; MIR domain; 2.
DR PROSITE; PS50188; B302_SPRY; 3.
DR PROSITE; PS50919; MIR; 4.
PE 4: Predicted;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Calcium channel {ECO:0000256|ARBA:ARBA00022673};
KW Calcium transport {ECO:0000256|ARBA:ARBA00022568};
KW Calmodulin-binding {ECO:0000256|ARBA:ARBA00022860};
KW Ion channel {ECO:0000256|ARBA:ARBA00023303};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Ligand-gated ion channel {ECO:0000256|ARBA:ARBA00023286};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Sarcoplasmic reticulum {ECO:0000256|ARBA:ARBA00022951};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 4118..4137
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 4345..4365
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 4412..4430
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 4554..4579
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 4602..4624
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 4683..4706
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 89..144
FT /note="MIR"
FT /evidence="ECO:0000259|PROSITE:PS50919"
FT DOMAIN 151..196
FT /note="MIR"
FT /evidence="ECO:0000259|PROSITE:PS50919"
FT DOMAIN 204..258
FT /note="MIR"
FT /evidence="ECO:0000259|PROSITE:PS50919"
FT DOMAIN 264..321
FT /note="MIR"
FT /evidence="ECO:0000259|PROSITE:PS50919"
FT DOMAIN 588..786
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000259|PROSITE:PS50188"
FT DOMAIN 1002..1198
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000259|PROSITE:PS50188"
FT DOMAIN 1244..1446
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000259|PROSITE:PS50188"
FT REGION 4041..4070
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4253..4309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4046..4070
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4270..4309
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4802 AA; 544008 MW; 42442F72EFF11132 CRC64;
CTRMGMCDDE VVLQCVACIQ KENRKFCLAA EGLGNRLCYL EPTSEAKYVP PDLCICTFVL
EQSLSVRALQ EMLAKSGQNS EGAAQSGGHK TLLYGHAILL RHSFSSMYLA CLKTSRSQTD
KLSFDVGLQE DSTGEACWWT IHPASKQRSE GEKVRIGDDL ILVSVSSERY LHLSISSGNI
QVDASFMQTL WNVHPICSGS NIEEGYLLGG HVMRLFHGHD EVVTIPGSDQ SEEQQRIVNY
ETGKAGAKAR SLWRLEPLRI SWSGSHIRWG QPFRLRHLTT GHYLALTEDR GLVLQDRERS
DTVATAFCFR ASKEKLEQSP KRDIEGMGVA EIKYGDSICF IMHVATGLWL SYQAPDVKSA
RLGPLKRRAC LHSEGHMDDG LTLQRCQHEE SRAARIIRNT TLLFKRFIRD LDCLGVKNRA
LVALPVEEVL QTLNDLITYF QLPDAELEHE ERQIKLRSLK NRQNLFKQEG MLTLVSNCID
RLNVYNSAAH FGECAGSEAG AAWKDILNLL YELLAALIRG NRNNCTQFSN NLDWLVSKLE
RLESSSGILE VLHCILIESP EALNIIQKGH IKSIISLLYK HGRNHKILDV LCSLCVCNGV
AVRTNQNLIC DHLLPKRDLL LQTQLVNDVQ SMRPNIFLGV SEGSAQYKKW YYELMIDQVD
HFVTSEPSHL RVGWANTKGY APYPGGGEGW GGNGVGDDLY SYGFDGLHLW SGRIPRAVAS
MNQHILTSED VVSCCLDLGA PSISFRINGQ PVQGMFENFN TDGLFFPVVS FSAGVKVRFL
LGGRHGDFKF LPPSSYAPCY EALLPKEKMK VEPVKEYKRD VDGVRDLLGT TQFMSQASFI
PTPVETSQIV MPPHLEKVRD KLAENIHELW GMNKIELGWS YGKIRDDNKR QHPCLVDFSK
LPETEKNYNL QMSTETLKTL LALGCRIVHM NPNAEDSLKK IKLPKNYMMS NGYKPSPLDL
SDIKLTPGQE LLVDKLAENA HNVWAKDRIK QGWTYGIQQD LKSKRNPRLV PYALLDERTK
KSNRDSLREA IRTLIGYGYN IEPSDQEGAG QVSERLSIDK IRFFRVERTY AVKTGKWYFE
FEAVTGGDMR VGWARPGCKP DVELGTDELA FVFDGYRGHC LNMGSRLFGR CWHAGDVVGC
MINMEDKSMI FTLNGEILIT TKGSELCFTD FETEDGFIPV CSLGLSQVGR MNLGKDASTF
KYYTMCGLQE GFEPFAVNMN REVTMWFSKR LPTFVNVPKD HNHIAVTRID GTIDSPPCLK
VTHKTFGTQN SNADMVFCRL SMPVEFHSFF KISPVVDMNV DYSSITTYYH SVRVFAGQDP
ACVWVGWVTP DYHYYSNNFN LSKNRTVTVT LGDERGRVHE SVKRSNCYMV WGGDVTSTSH
TSGRSNVDLE IGCLIDLATG LLTFTVNGKE ISTSYQVEPN TKLFPAVFVR PTSPSLFQFE
LAKIKNAMPL SSAIFKSEHK NPVPQCPPRL DVQTINAVLW SRMPNSFLKV ETARVNERHG
WVVQCVEPLE MLAVHIPEEN RCVDVMELSE QEDMKKFHYH TLKLYCALCA LGNTRVAHAL
CSHLDQSQLL YTIDNQYLSG MLREGFYNVL ISIHLETAKE ARLMMKDEFV IPVTAETRSI
RLFSDASKKH LPPGVGLSTS LKPRLNFTPP CFISTKREHL YSPQIPLDAL KEKAILMLTE
AVQSGGHHIR DPVGGCVEHQ FVPILKLIST LLTMGVLGSE DVRKILLLID PNVFKEAREE
GATGTTDKEG LTGAEEKAVE AGEEEAIKEA QQPMEGLLEK RLPEPVKRQM CELLHYFCDC
ELKHRIEAIV SFSDSFVSKL QYNQKFRYNE LMLALNMSAA VTAKKTKEFR SPPQEQINML
LNFSMGEDCP CPADIQEELY DFHNQLQLHC GIPMEEDEDE QDTSIKGRLL MLVNRIKGQA
HKAEEPTKME QAAPSNLKEL ISQTMVSWSQ ECHIQDPELV RKMFSLLRRQ YDSVGELLRA
MRKTYTISAA SVQDTINLLA SLGQIRSLLS VRMGKEEEKL MIDGLGDIMN NKVFYQHPNL
MRILGMHETV MEVMVNVLGG DKSQEIAFPK MVASCCRFLC YFCRISRQNQ KAMFDHLSYL
LENSSVGLAS PAMRGSTPLD VAASSVMDNN GLALKEKPTL RPTGRECTDH LSYMICPMLL
AKGYPDIGWN PIEGERYLSF LRFAVFVNGE SVEENSSVVV KLLIRRPECF GPALRGEGGN
GLLAAMKEAI KISETPALDL PGSVQGVSSD EVVHMGNAIM SFYSALIDLL GRCAPEMHLI
NKGKGEALRI RAILRSLVPT EDLVGIISIP LKMPITNKDG SVTEPDMSAS FCPDHKAPMV
LFLDRVYGVE DQSFLLHLLE VGFLPDLRAA TSLDTEGLCT TETALAMNRY IGSAMLPLLT
RCAPLFAGTE HYATLIDSTL HTIYRLSKGR SLTKAQRDAI EECLLSVCKH LRPSMLQQLL
RRLVFDVPLL TDYCKMPLRL LTNHYEQNWK YYCLPSGWGS FGTASEDELL LTKKIFWGVF
DSLSQRKYDP ELFKMAMPCL SAIAGALPPD YVDASISTTV EKPVSVDAQG NFDPKPINTA
NIALPEKLEH FANKYADSNN HDSAKYDVLL GWKYGDCVDE KAKTHPQLRT YKGLTEKEKD
IYRWPIRESL KSMLAMGWSI DRTKDGESMS QQRETEKMRK ISQASQANGF NPSPIDTSNV
VLSRELQGMV EVVAENYHNI WAKKKKSDLG SRGGGTHPLL VPYDTLTAKE KARDREKAQD
LFRFLLINGY SITRGLKDLE QDSSSMEKRF AYKFLKKLLK CVDSAQEFIT HLEAMAASGK
TDRSPHEQEI KFFAKVLLPL IDQYFKNHSL YFLSSPNKNL SSSGYASNKE KEMVTSLFCK
LAALVRHRIS LFGSDSTTMV SCLHILAHTL DTRTVMKSGS EQVRVGLRTF FENAAEDLEK
TFENLKLGKF THSRSQMKGV SQNINYTTAA LLPILTALFE HITQHHFGVD LLLDDVQVSC
YRILTSLYAL GTGKNIYVER QLPALGQCLA TLAGALPVAF LEPQLNPYNP CSVFNTKSAR
ERATLGIQDS VEEMCPGMPR LDRLMKDIND MAESGARYTE MPHVIEVVLP MLCNYLSYWW
ERGPENQPAS GGSICCTTVT SEHLSLILGN ILKILNNNLG IDEASWMKRI AVYVQPIISK
ARADLLKSHF LPTLEKLKKK TVKVVAEEEL LKADCKGDTQ EAELLILDEF AVLCRDLYAF
YPMLIRYVDN NRSRWLKEPD SDSNDLFRMV AEIFILWCKS HNFKREEQNF VVQNEINNLS
FLTGDNKTKM SKSGGQDQER KHKKRRGEFY SIQTSLIVAA LKKMLPIGLN MCTPGDQELI
SLAKTRYLMK DTDDEVKDHI QQNLHLREKS DDPAVRWQLN LYKDIVMRTE GLSDPEKVVD
RIQRISAAVF NLEQVEQPLR SKKCVWQKLL SKQRKRAVVA CFRMAPLYNL PRHRSINLFL
LAYQRIWIET EEYSFEEKLV QDLAPKVEEE EEEEVRKQPD PLHQLILHFS HNALTECRSG
ACHNVLQEKE MEKQKMLYQQ ARLHDRGAAE MVLQMISASK GRLGATVTVT LKLGISILNG
GNILVQQKML DYLKEKRDVG FFKSLSGLMM SCSVLDLNAF ERQNKAEGLG MVTEEGSSKC
SKVLQNDEFT KDLFRFLQLL CEGHNNDFQN FLRTQTGNTT TVNIIISTVD YLLRLQESIS
DFYWYYSGKD VIDEAGQRNF SKALAVAKQV FNSLTEYIQG PCIGNQQSLA HSRLWDAVVG
FLHVFANMQM KLSQDSSQIE LLKELLDLQK DMVVMMLSLL EGNVVNGTIG KQMVDTLVES
SSNVEMILKF FDMFLKLKDL TTSDSFKEYD PESKGVISKK DFQKSMENQK QYSQSEIEFL
LSCAEADEND MFNYEQFVER FHEPAKDIGF NVAVLLTNLS EHMPHDARLS TFLDLAESVL
SYFEPYLGRI EIMGGAKRIE RVYFEISESS RTQWEKPQVK ESKRQFIFDV VNEGGESEKM
ELFVNFCEDT IFEMQLASQI SEPDPVERPE EEEDDIQTMM ENQEEEDEES AMMESSSAFT
IACISMRKSL CHFRQLLTLK SMRRQFRKFR KMSVKEMVRG FFSFFWMIIT GLFHFVYSLI
WGFFHILWTT MFGGGLVEGA KNMKVTDILG NMPDPTQFGI HGDVLETEKM EASEASALAE
MGQMAQGEAA EADLMAELLN IQPKKEGKHG AEPGLGDVSE VVVGDAPSIA SAVKQKKAQM
AANTRAVNGD YRSDTEKGDS EDGEKKDHDK TRDEAPPELS VEEKKAPKKR LGLKKEAPEA
FMASFFAGLE VYQTKMLNYL ARNFYNLRFL ALFVAFAINF ILLFYKVTGD YSDDEDPWNG
RGRVGEEEDE GALEYFVLQE STGYMAPTLR CLAILHTIIS FLCVVGYYYL KVPLVVFKRE
KEIARKLEFA GLYITEQPSD DDIKGQWDRL VINTPSFPNN YWDKFVKRKV IKKYGDLYGA
ERIAELLGLD KSALDFNPTE ETVAKEASLV SWLSSIDTKY HVWKMGVVFT DNSFLYLVWY
TTMSILGHYN NFFFAAHLLD IAMGFKTLRT ILSSVTHNGK QLVLTVGLLA VVVYLYTVVA
FNFFRKFYNK SEDEDEPDMK CDDMMTCYLF HMYVGVRAGG GIGDEIEDPA GDPYELYRIL
FDITFFFFVI VILLAIIQGL IIDAFGELRD QQEQVKEDME TKCFICGIGN DYFDTTPHGF
ETHTLQEHNL ANYLFFLMYL INKDETEHTG QESYVWKMYQ ERCWDFFPAG DCFRKQYEDQ
LG
//
