ID I4G1B9_MICAE Unreviewed; 546 AA.
AC I4G1B9;
DT 05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT 05-SEP-2012, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=(R)-citramalate synthase {ECO:0000256|ARBA:ARBA00022325};
DE EC=2.3.3.21 {ECO:0000256|ARBA:ARBA00034330};
GN ORFNames=MICAC_2490006 {ECO:0000313|EMBL:CCI01730.1};
OS Microcystis aeruginosa PCC 9443.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Microcystaceae; Microcystis.
OX NCBI_TaxID=1160281 {ECO:0000313|EMBL:CCI01730.1, ECO:0000313|Proteomes:UP000003480};
RN [1] {ECO:0000313|EMBL:CCI01730.1, ECO:0000313|Proteomes:UP000003480}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=9443 {ECO:0000313|EMBL:CCI01730.1,
RC ECO:0000313|Proteomes:UP000003480};
RA Genoscope - CEA;
RL Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+);
CC Xref=Rhea:RHEA:19045, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30934, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.21;
CC Evidence={ECO:0000256|ARBA:ARBA00034270};
CC -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-
CC oxobutanoate from pyruvate: step 1/3. {ECO:0000256|ARBA:ARBA00004743}.
CC -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC family. {ECO:0000256|ARBA:ARBA00006154, ECO:0000256|RuleBase:RU003523}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCI01730.1}.
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DR EMBL; CAIJ01000167; CCI01730.1; -; Genomic_DNA.
DR RefSeq; WP_002767151.1; NZ_HE972962.1.
DR AlphaFoldDB; I4G1B9; -.
DR HOGENOM; CLU_022158_7_0_3; -.
DR UniPathway; UPA00047; UER00066.
DR Proteomes; UP000003480; Unassembled WGS sequence.
DR GO; GO:0043714; F:(R)-citramalate synthase activity; IEA:RHEA.
DR GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:InterPro.
DR GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:InterPro.
DR CDD; cd07941; DRE_TIM_LeuA3; 1.
DR Gene3D; 1.10.238.260; -; 1.
DR Gene3D; 3.30.160.270; -; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR005675; Citramal_synthase.
DR InterPro; IPR036230; LeuA_allosteric_dom_sf.
DR InterPro; IPR000891; PYR_CT.
DR NCBIfam; TIGR00977; citramal_synth; 1.
DR PANTHER; PTHR43538:SF1; (R)-CITRAMALATE SYNTHASE; 1.
DR PANTHER; PTHR43538; ALPHA-IPM SYNTHASE/HOMOCITRATE SYNTHASE; 1.
DR Pfam; PF00682; HMGL-like; 1.
DR Pfam; PF08502; LeuA_dimer; 1.
DR SMART; SM00917; LeuA_dimer; 1.
DR SUPFAM; SSF110921; 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain; 1.
DR SUPFAM; SSF51569; Aldolase; 1.
DR PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR PROSITE; PS50991; PYR_CT; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:CCI01730.1};
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00023304};
KW Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304};
KW Isoleucine biosynthesis {ECO:0000256|ARBA:ARBA00022624};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003523}.
FT DOMAIN 7..277
FT /note="Pyruvate carboxyltransferase"
FT /evidence="ECO:0000259|PROSITE:PS50991"
SQ SEQUENCE 546 AA; 60540 MW; FB87B923E7002D37 CRC64;
MNNGKRIWVY DTTLRDGAQR EGISLSLEDK IKIARELDRM GIPFIEGGWP GANPRDGQFF
RKMHEEPLKQ AELVAFCSTR RPHVEAREDP MLTAILAART RWVTIFGKSW DLHVTEGLKT
SLEENLAMIG DTIEYLRCQG RRVIYDAEHW FDGYKNHPEY ALLTLKTARD AGAEWLVFCD
TNGGTLPQEI APIVEKVREQ LGIDPDEENA PQLGIHAHND AGTAVANSLA AVNEGARMIQ
GTINGYGERC GNANLCTLIP NLQLKMGFSC LEENQLGRLT GTSRKISEMV NLAPDDHAPF
VGRSAFAHKG GIHVSAVAKN PLTYEHINPE AIGNQRRIVI SDQSGLSNVL AKARSFGIDL
NKDDPTCRQI LERLKILESQ GYQFEAAEAS FELLMREALG QRSHLFELKG FQVYCDMLRD
SGNAIATIKV QVKEEDLLEV AEGNGPVAAL DMALRKALVK FYPEIANFHL TDYKVRILDS
GSGTAAKTRV LIESSNGQQR WTTVGVSSNI LEASYQAVVE GIEYGLYLLQ NNKLELSGQF
CPLGII
//
