ID I4HQ27_MICAE Unreviewed; 546 AA.
AC I4HQ27;
DT 05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT 05-SEP-2012, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=MICAG_2370001 {ECO:0000313|EMBL:CCI24151.1};
OS Microcystis aeruginosa PCC 9808.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Microcystaceae; Microcystis.
OX NCBI_TaxID=1160284 {ECO:0000313|EMBL:CCI24151.1, ECO:0000313|Proteomes:UP000005291};
RN [1] {ECO:0000313|EMBL:CCI24151.1, ECO:0000313|Proteomes:UP000005291}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=9808 {ECO:0000313|EMBL:CCI24151.1,
RC ECO:0000313|Proteomes:UP000005291};
RA Genoscope - CEA;
RL Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCI24151.1}.
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DR EMBL; CAIN01000154; CCI24151.1; -; Genomic_DNA.
DR AlphaFoldDB; I4HQ27; -.
DR HOGENOM; CLU_015410_1_0_3; -.
DR Proteomes; UP000005291; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0006306; P:DNA methylation; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF02384; N6_Mtase; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 225..355
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 361..465
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 546 AA; 61403 MW; D90913A475F3384F CRC64;
MTGYKDETGK IQHEIGVYYV SKSNFGVGQI VGEFSDFSFL APKNFDQFIE KVKTLNLSQE
EIDKLKEQRE KEIDASLVKL NNDIYQNEKG LGENDRVYLV AASIIATIGI PGKVSPLEKS
DLKSSTEKGN TDGEIIVRKI TAFLGEKEIP AEKKDLIIRT LSNTLLTENI NKVENGESQL
KRVFSKIVDD LGIYYKIGLT TDFTGKLFNE MYSWLGFSQD KLNDVVLTPS YIATLLVKLA
RVNKDSYVWD FAAGSAGLLV AAMNEMLIDA KDNIKSPEEL TQKEIKIKTE QLLGLELLSS
VYMLAILNMI LMGDGSSNIL NKDSIKDFDG NYVFGQTNSK FPADAFVLNP PYSANGNGMN
FVEKALAMMN KGYAAIIIQN SAGSGRAKEY NKKILEKHTL LASIKMPIDL FIGKSSVQTN
VYVFKVNEKH HKDEIVKFID FSNDGYTRTN RKKSSNNLKD TDSAKERYEE LVNLVRFGKS
KLNIFTEKEY YEGTIDPKNG SDWNQTAPID TKPTLQDFKK TVSDYLAWEV SNILRQQSTE
EERLGK
//
