ID I7J8F7_METBM Unreviewed; 419 AA.
AC I7J8F7;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE SubName: Full=Transposase {ECO:0000313|EMBL:CCJ36058.1};
GN OrderedLocusNames=BN140_1135 {ECO:0000313|EMBL:CCJ36058.1};
OS Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2)
OS (Methanogenium bourgense).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
OX NCBI_TaxID=1201294 {ECO:0000313|EMBL:CCJ36058.1, ECO:0000313|Proteomes:UP000009007};
RN [1] {ECO:0000313|Proteomes:UP000009007}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43281 / DSM 3045 / OCM 15 / MS2
RC {ECO:0000313|Proteomes:UP000009007};
RX PubMed=22965103; DOI=10.1128/JB.01292-12;
RA Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A., Boelter J.,
RA Szczepanowski R., Blom J., Jaenicke S., Konig H., Puhler A., Schluter A.;
RT "Complete genome sequence of the hydrogenotrophic, methanogenic archaeon
RT Methanoculleus bourgensis strain MS2T, isolated from a sewage sludge
RT digester.";
RL J. Bacteriol. 194:5487-5488(2012).
CC -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC family. {ECO:0000256|ARBA:ARBA00008761}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the transposase 2
CC family. {ECO:0000256|ARBA:ARBA00011044}.
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DR EMBL; HE964772; CCJ36058.1; -; Genomic_DNA.
DR RefSeq; WP_014867034.1; NC_018227.2.
DR AlphaFoldDB; I7J8F7; -.
DR STRING; 1201294.BN140_1135; -.
DR GeneID; 13355641; -.
DR KEGG; mbg:BN140_1135; -.
DR PATRIC; fig|1201294.9.peg.1254; -.
DR HOGENOM; CLU_032903_16_5_2; -.
DR BioCyc; MBOU1201294:BN140_RS05660-MONOMER; -.
DR Proteomes; UP000009007; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR InterPro; IPR010095; Cas12f1-like_TNB.
DR InterPro; IPR001959; Transposase.
DR InterPro; IPR021027; Transposase_put_HTH.
DR NCBIfam; NF040570; guided_TnpB; 1.
DR NCBIfam; TIGR01766; IS200/IS605 family accessory protein TnpB-like domain; 1.
DR PANTHER; PTHR30405:SF20; RNA-GUIDED DNA ENDONUCLEASE INSQ-RELATED; 1.
DR PANTHER; PTHR30405; TRANSPOSASE; 1.
DR Pfam; PF12323; HTH_OrfB_IS605; 1.
DR Pfam; PF01385; OrfB_IS605; 1.
DR Pfam; PF07282; OrfB_Zn_ribbon; 1.
PE 3: Inferred from homology;
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Reference proteome {ECO:0000313|Proteomes:UP000009007};
KW Transposition {ECO:0000256|ARBA:ARBA00022578};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 1..45
FT /note="Transposase putative helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12323"
FT DOMAIN 175..293
FT /note="Probable transposase IS891/IS1136/IS1341"
FT /evidence="ECO:0000259|Pfam:PF01385"
FT DOMAIN 312..377
FT /note="Cas12f1-like TNB"
FT /evidence="ECO:0000259|Pfam:PF07282"
SQ SEQUENCE 419 AA; 48276 MW; 76234094B2DF1645 CRC64;
MHITTKIKIQ VTPDQADVLW HLSEKCRLLY NFALAERNQN YQENKDRPDK QYIGYVQQAN
ALPDLKKKYP EYRWVYSKVL QTTLKSLDDN FKSFFALRQN GDEDANPPGF KGKDHFCTLR
YNQSGFAIAG GRIRFSHFYN EVPLEFDLPE VQTFLNVKQV DLFYDDLKKA YYVSIVHEVQ
PDQKYVDNGF YQAWDLGVTK HVAVNTSGKF LEVRNARPDR YWNPKIDAVQ ARRDHCKKKS
RKWQRLNRAK RTMERKRSNQ LNDFQHKLSR NLVENTRAAT ILVGDLSVKQ MPKSKQATPG
LNRATQNAGH LSRFIGFLTY KAALAGKRVI KVDEQYTTKT CCVCGAVHEM PLWKRVMDCA
CGNCIDRDHN SAVNIMVRFL SQNALWAGYR QFAGNLRQTG PRIPVVPGHS QEAPCASGG
//
