ID I7L004_METBM Unreviewed; 306 AA.
AC I7L004;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000256|HAMAP-Rule:MF_01081};
DE EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE Short=Psi55 synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000256|HAMAP-Rule:MF_01081};
GN Name=truB {ECO:0000256|HAMAP-Rule:MF_01081};
GN OrderedLocusNames=BN140_1642 {ECO:0000313|EMBL:CCJ36565.1};
OS Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2)
OS (Methanogenium bourgense).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
OX NCBI_TaxID=1201294 {ECO:0000313|EMBL:CCJ36565.1, ECO:0000313|Proteomes:UP000009007};
RN [1] {ECO:0000313|Proteomes:UP000009007}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43281 / DSM 3045 / OCM 15 / MS2
RC {ECO:0000313|Proteomes:UP000009007};
RX PubMed=22965103; DOI=10.1128/JB.01292-12;
RA Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A., Boelter J.,
RA Szczepanowski R., Blom J., Jaenicke S., Konig H., Puhler A., Schluter A.;
RT "Complete genome sequence of the hydrogenotrophic, methanogenic archaeon
RT Methanoculleus bourgensis strain MS2T, isolated from a sewage sludge
RT digester.";
RL J. Bacteriol. 194:5487-5488(2012).
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP-
CC Rule:MF_01081}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01081};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01081}.
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DR EMBL; HE964772; CCJ36565.1; -; Genomic_DNA.
DR RefSeq; WP_014867539.1; NC_018227.2.
DR AlphaFoldDB; I7L004; -.
DR STRING; 1201294.BN140_1642; -.
DR GeneID; 13354762; -.
DR KEGG; mbg:BN140_1642; -.
DR PATRIC; fig|1201294.9.peg.1803; -.
DR HOGENOM; CLU_032087_3_0_2; -.
DR BioCyc; MBOU1201294:BN140_RS08210-MONOMER; -.
DR Proteomes; UP000009007; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd21148; PUA_Cbf5; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 2.30.130.10; PUA domain; 1.
DR HAMAP; MF_01081; TruB_arch; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR026326; TruB_arch.
DR InterPro; IPR032819; TruB_C.
DR NCBIfam; TIGR00425; CBF5; 1.
DR PANTHER; PTHR23127; CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5; 1.
DR PANTHER; PTHR23127:SF0; H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
DR PROSITE; PS50890; PUA; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|HAMAP-Rule:MF_01081, ECO:0000313|EMBL:CCJ36565.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000009007};
KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_01081}.
FT DOMAIN 1..29
FT /note="Dyskerin-like"
FT /evidence="ECO:0000259|SMART:SM01136"
FT DOMAIN 214..288
FT /note="PUA"
FT /evidence="ECO:0000259|SMART:SM00359"
FT ACT_SITE 47
FT /note="Nucleophile"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01081"
SQ SEQUENCE 306 AA; 32631 MW; A7ECBAD5407D97F9 CRC64;
MTGQEEIPVP GSGIVVIDKP RGPSSHQVTA WVGDILGRRV GHAGTLDPQV SGVLVVMFGG
AVRLAPVLLS HNKEYVCLMR LHGDVSREAV DRVAEEFVGR VYQRPPRKSA VKRNLRIRKI
QEIEVLDMEG RLVLFRVRCD AGTYIRTLCV HMGYTLGVCA HMQELRRIRS GPFDETAAVS
LHELADAAAA AREGNLVPLQ QMILSPGAAV ADLPKVVIRD TAVDAVCHGA VLAGVGVTGR
EGGFGKGEVV AIMTGRGELV GLGKALVSSS ALKPGEPGLV VAPTTVLMQP GTYPRGWKTH
AGEPRR
//
