ID I8W652_9BACE Unreviewed; 1035 AA.
AC I8W652;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230, ECO:0000256|RuleBase:RU361154};
GN ORFNames=HMPREF1062_01704 {ECO:0000313|EMBL:EIY34120.1};
OS Bacteroides cellulosilyticus CL02T12C19.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=997874 {ECO:0000313|EMBL:EIY34120.1, ECO:0000313|Proteomes:UP000003741};
RN [1] {ECO:0000313|EMBL:EIY34120.1, ECO:0000313|Proteomes:UP000003741}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CL02T12C19 {ECO:0000313|EMBL:EIY34120.1,
RC ECO:0000313|Proteomes:UP000003741};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Zitomersky N.L., Coyne M.J.,
RA Comstock L.E., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Chapman S.B.,
RA Gearin G., Goldberg J., Griggs A., Gujja S., Hansen M., Heiman D.,
RA Howarth C., Larimer J., Lui A., MacDonald P.J.P., McCowen C.,
RA Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M., Roberts A.,
RA Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Bacteroides cellulosilyticus CL02T12C19.";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU361154};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIY34120.1}.
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DR EMBL; AGXG01000034; EIY34120.1; -; Genomic_DNA.
DR RefSeq; WP_007216486.1; NZ_JH724086.1.
DR AlphaFoldDB; I8W652; -.
DR PATRIC; fig|997874.3.peg.1742; -.
DR HOGENOM; CLU_002346_0_2_10; -.
DR OrthoDB; 9801077at2; -.
DR Proteomes; UP000003741; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154}.
FT DOMAIN 753..1028
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
SQ SEQUENCE 1035 AA; 118689 MW; A52B582A67CBCC65 CRC64;
MKKRLFSLFA LAIGLQTVAL SQKNEWRDPE VNAVNRAPMH ANYFAYESYD AARTGAKEES
DNFLSLNGMW KFNWVRNADQ RPDDFYQLGY NDSAWDELKV PAVWELNGYG DPIYVNVGYP
WKNQFRTDPP KIPVANNHVG SYRKEIVIPA EWKNKQIFVH FGSVTSNIYL WVNGKYVGYS
EDSKLEAEFD LSGYLKPGKN LIAFQVFRWC DGSYLEDQDF FRFSGVGRDC YLYARNKTCI
QDIRVTPDLD QQYTNGSLDI ALQVKGRGTV ELELSDAKGN VVANTRLNGS GSLSTVMEVK
NPLKWSAEMP NLYCLTATLK NGNDILEVIP VKVGFRKVEI KDAQLLVNGQ PVLIKGANRH
EMDPDYGYVV SRERMLQDIR IMKQFNINAV RTCHYPDDNL WYELCDEYGL YVVAEANVEA
HGMLYTNNQL SKHASFAKAH LERNQRNVQR SYNHPSVIIW SLGNETGPGP NFEACYRWIK
AEDATRPVQY EQAGHDYYTD IFCPMYLWYS ACEDYAKSNA TKPLIQCEYA HAMGNSMGGF
KEYWDLIRKY PKFQGGFIWD FVDQSVRWKN KDGIEIYAYG GDFNKYDGSD NNFCDNGLIS
PDRVPNPHMY EVGYFYQSIW THPVNLQNGE IEIFNENFFR DLSAYYLDWQ LLADGELVEA
GTVSNLNVAP QQKAKLKLDI SDVNSYKDKE LLLNVSYKLK KAETLLSPGF TVAKAQMSVI
PYKAPDMALV NVKKANIESV APSVNNNDGN YLIIEGEDFI IEFAKNNGFL SRYKVAGKEL
MNDGGQLVPN FWRAPTDNDY GARLQHKYRV WLNPKLKRTS FTNKQENGMI VVEAGYEMPD
VSAKLYLTYV INNTGEIKVT QKMVAGEAEK VPDMFRFGMQ MQMPDEFYRI NYYGRGPVEN
YSDRNHATDL GIYRQTVAEQ FYPYIRPQET GTKTDIRWWR QLNEAGSGLQ FVAEAPFSAS
ALNYTIESLD DGLNKDQRHS PEVIPVDYTN LCIDKAQLGL ACENSWGAIA YPQYRLPYGN
YEFSFIMKPV FNKVY
//
