ID I9L0G7_9SPHN Unreviewed; 869 AA.
AC I9L0G7;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE SubName: Full=Nitrate reductase {ECO:0000313|EMBL:EIZ77724.1};
GN ORFNames=WSK_3734 {ECO:0000313|EMBL:EIZ77724.1};
OS Novosphingobium sp. Rr 2-17.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Novosphingobium.
OX NCBI_TaxID=555793 {ECO:0000313|EMBL:EIZ77724.1, ECO:0000313|Proteomes:UP000004732};
RN [1] {ECO:0000313|EMBL:EIZ77724.1, ECO:0000313|Proteomes:UP000004732}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RR2-17 {ECO:0000313|Proteomes:UP000004732};
RX PubMed=22933764; DOI=10.1128/JB.01159-12;
RA Gan H.M., Chew T.H., Hudson A.O., Savka M.A.;
RT "Genome Sequence of Novosphingobium sp. Strain Rr 2-17, a Nopaline Crown
RT Gall-Associated Bacterium Isolated from Vitis vinifera L. Grapevine.";
RL J. Bacteriol. 194:5137-5138(2012).
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. NasA/NapA/NarB subfamily.
CC {ECO:0000256|ARBA:ARBA00008747}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIZ77724.1}.
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DR EMBL; AKFJ01000118; EIZ77724.1; -; Genomic_DNA.
DR RefSeq; WP_008996656.1; NZ_AKFJ01000118.1.
DR AlphaFoldDB; I9L0G7; -.
DR PATRIC; fig|555793.3.peg.3669; -.
DR eggNOG; COG0243; Bacteria.
DR OrthoDB; 9816402at2; -.
DR Proteomes; UP000004732; Unassembled WGS sequence.
DR GO; GO:1990204; C:oxidoreductase complex; IEA:UniProt.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd02791; MopB_CT_Nitrate-R-NapA-like; 1.
DR CDD; cd02754; MopB_Nitrate-R-NapA-like; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.40.50.740; -; 1.
DR Gene3D; 2.20.25.90; ADC-like domains; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.228.10; Dimethylsulfoxide Reductase, domain 2; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR041957; CT_Nitrate-R-NapA-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR PANTHER; PTHR43105:SF9; NITRATE REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15190)-RELATED; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF04879; Molybdop_Fe4S4; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000004732}.
FT DOMAIN 2..58
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 869 AA; 93867 MW; AA6B4141E737D4DB CRC64;
MVNSIRTTCA YCGVGCGIAA TPTGGRAVTI RGDEAHPANS GRLCSKGTHL GETMGLEGRL
LHPMIGDKRA SWDKALDLVA KRFRETIARH GPDSVAFYVS GQLLTEDYYV ANKLMKGFIG
SANIDTNSRL CMSSAVAGHT RAFGEDIVPA SYEDLDEADL IVLVGSNTAW CHPVVYQRIQ
AARAARGTKL VVIDPRRTET CEGADLHLAV RPGSDVALMN GLLAWMDQAG LLDTDFLRDR
VNAPDGFWDA IRTGHDLWST AKACDVPPAA LQQFYELFAA TSRTVTLFSQ GVNQSIRGTD
QVNAIINVHL ATNRIGKPGA APFSITGQPN AMGGREVGGL ASTLAAHMDF APEHVARVGR
FWSAPNMATK PGLKAVDLFR AIGEGRIKAL WVMATNPAVS LPDGNRVREA LAACPFVVVS
DVMATTDTTV HAHVRLPAAA WGEKDGTVTN SDRTVSRQRA LLPLPGDAKP DWWIVTQVAR
RMGWQDTFAY DRPADIWREH ARLTAYRNDG ARLLNLQAQA AISNEAYDAM TPFRWGGTPF
ADGRFSTPDR KARLVPVKQM DLQAPLREWP MTLNTGRYRD QWHTMTRTGL SPKLARHREE
PLVEVHPHDA AALGIADGDL ARLSTPQGES LFRARISDGQ RLGEVFTPIH WTDQQSSGGR
TGLLPRPLVD PHSGQPGFKS TPVRVEKVVT EWKGFLILRG DEPARVPCLW MTRITVPGGA
LYEVAGNGDP ARLDTCLPKG DRIEAVDATR GTRRVAMIRD GRLAGVLIVT RTGLLPSREW
LIGQLAVESV GASVLAARGP GDQPDKGAVV CVCFDIGLNQ IVAAIRDQGL VDVPAIGQAI
GAGTNCGSCR PALANLIMQS SQETLRAAQ
//
