ID I9UWF0_9BACE Unreviewed; 1144 AA.
AC I9UWF0;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230, ECO:0000256|RuleBase:RU361154};
GN ORFNames=HMPREF1074_01502 {ECO:0000313|EMBL:EIY87131.1};
OS Bacteroides xylanisolvens CL03T12C04.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=997892 {ECO:0000313|EMBL:EIY87131.1, ECO:0000313|Proteomes:UP000003566};
RN [1] {ECO:0000313|EMBL:EIY87131.1, ECO:0000313|Proteomes:UP000003566}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CL03T12C04 {ECO:0000313|EMBL:EIY87131.1,
RC ECO:0000313|Proteomes:UP000003566};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Zitomersky N.L., Coyne M.J.,
RA Comstock L.E., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Chapman S.B.,
RA Gearin G., Goldberg J., Griggs A., Gujja S., Hansen M., Heiman D.,
RA Howarth C., Larimer J., Lui A., MacDonald P.J.P., McCowen C.,
RA Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M., Roberts A.,
RA Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Bacteroides xylanisolvens CL03T12C04.";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU361154};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIY87131.1}.
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DR EMBL; AGXE01000008; EIY87131.1; -; Genomic_DNA.
DR AlphaFoldDB; I9UWF0; -.
DR PATRIC; fig|997892.3.peg.1539; -.
DR HOGENOM; CLU_002346_1_1_10; -.
DR Proteomes; UP000003566; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR023232; Glyco_hydro_2_AS.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154}.
FT DOMAIN 861..1141
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
SQ SEQUENCE 1144 AA; 130686 MW; A2EB10BBA2A82C32 CRC64;
MLIQYSYFNI RYSNINRMKL SSFPIKERGT KCPFVRLLDM KKKERMKIYT LLLGVLFVSP
IQAQTMHDWE NHHVLQINRE PARAAFTPFS VQKGDGSISL DGTWKFRWTP VPNERVVNFY
QTNFNDKDWT DFPVPANWEV NGYGTPIYVS AGYPFKIDPP RVMGEPKTDY TTYKERNPVG
QYRRTFVLPA GWEANGQTFL RFEGVMSAFY VWINGERVGY SQGSMEPSEF NVTKYLKSGE
NQISLEVYRY SDGSYLEDQD FWRFGGIHRS IHLIHTPDIH VRDYAVRTLP ASAGDYEDFI
LQIDPQFSVY RGMTGKGYVL QGVLKDASGK EVATLKGDVE DILDLEHKAS RMNEWYPQRG
PRKMGRLSAI IKSPERWTAE TPYLYKLHLT LQNEEGKVVE QIEQAVGFRS VEIKKGQLLV
NGNPVRFRGV NRHEHDPRTA RVMSEERMLQ DILLMKQANI NAVRTSHYPN VSRWYELCDS
LGLYVMDEAD IEEHGLRGTL ASTPDWHAAF MDRAVRMAER DKNYPCIVMW SMGNESGYGP
NFAAISAWLH DFDPTRLVHY EGAQGVDGNP DPKTVDVISR FYTRVKQEYL NPGIAEGEDK
ERAENARWER LLEIAERTND DRPVMTSEYA HSMGNALGNF KEYWDEIYSN PRMLGGFIWD
WVDQGIYKTL PDGRTMVAYG GDFGDKPNLK AFCFNGLLMS DRKTTPKYWE VKKVYEPVEL
KMENGKLKVT NRNHHIDLSS YRCLWTVSID GKQKEQGEFT LPEIAPGESE TIDLPAFRSL
KGTYSLSNKN EKVSKSNKKT EKTLSDCQLK VSIVLKSDAL WAKAGHEVAC EQFCLQKGDL
ASADLINKGA LQVKEDDKSL LISGRSFSVQ WEKKVNGSMT SLIYKDKEML AHSDDFPVQP
VTQVFRAPTD NDKSFGNWLA KDWKLHGMDH PQINLESFHH EKRADGAVIV RIQTSNLYKE
GKVVTTSVYT VFSDGTIDLK TTFLPQGVLP EIPRLGTAFC LAPAYDTFTW YGRGPQDNYP
DRKTSAMIGL WKGSVAEQYV HYPRPQDSGN KEEVHYLTLT DKQNKGIRVD AVENAFSASA
LHYTVQDIYE ETHDCNLKPR AEVILSMDAA VLGLGNSSCG PGVLKKYAIE KKEHTLHLRI
SSKQ
//
