ID ISP6_SCHPO Reviewed; 467 AA.
AC P40903;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 27-MAR-2024, entry version 151.
DE RecName: Full=Sexual differentiation process putative subtilase-type proteinase isp6;
DE EC=3.4.21.-;
GN Name=isp6; ORFNames=SPAC4A8.04;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7954893; DOI=10.1007/bf00326301;
RA Sato S., Suzuki H., Widyastuti U., Hotta Y., Tabata S.;
RT "Identification and characterization of genes induced during sexual
RT differentiation in Schizosaccharomyces pombe.";
RL Curr. Genet. 26:31-37(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- DEVELOPMENTAL STAGE: Transcribed specifically during sexual
CC development.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; D14063; BAA03149.1; -; mRNA.
DR EMBL; CU329670; CAB11474.1; -; Genomic_DNA.
DR PIR; S45493; S45493.
DR RefSeq; NP_593815.1; NM_001019245.2.
DR AlphaFoldDB; P40903; -.
DR SMR; P40903; -.
DR BioGRID; 279529; 10.
DR STRING; 284812.P40903; -.
DR MEROPS; S08.A54; -.
DR iPTMnet; P40903; -.
DR MaxQB; P40903; -.
DR PaxDb; 4896-SPAC4A8-04-1; -.
DR EnsemblFungi; SPAC4A8.04.1; SPAC4A8.04.1:pep; SPAC4A8.04.
DR GeneID; 2543097; -.
DR KEGG; spo:SPAC4A8.04; -.
DR PomBase; SPAC4A8.04; isp6.
DR VEuPathDB; FungiDB:SPAC4A8.04; -.
DR eggNOG; KOG1153; Eukaryota.
DR HOGENOM; CLU_011263_1_4_1; -.
DR InParanoid; P40903; -.
DR OMA; HPHVIAV; -.
DR PhylomeDB; P40903; -.
DR Reactome; R-SPO-8866427; VLDLR internalisation and degradation.
DR Reactome; R-SPO-8964038; LDL clearance.
DR PRO; PR:P40903; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0000324; C:fungal-type vacuole; IDA:PomBase.
DR GO; GO:0000328; C:fungal-type vacuole lumen; EXP:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0031906; C:late endosome lumen; EXP:PomBase.
DR GO; GO:0004175; F:endopeptidase activity; IMP:PomBase.
DR GO; GO:0008233; F:peptidase activity; IMP:PomBase.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IDA:PomBase.
DR GO; GO:0006914; P:autophagy; IMP:PomBase.
DR GO; GO:0000747; P:conjugation with cellular fusion; IMP:PomBase.
DR GO; GO:0007033; P:vacuole organization; IMP:PomBase.
DR GO; GO:0031638; P:zymogen activation; IMP:PomBase.
DR CDD; cd04077; Peptidases_S8_PCSK9_ProteinaseK_like; 1.
DR Gene3D; 3.30.70.80; Peptidase S8 propeptide/proteinase inhibitor I9; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR InterPro; IPR034193; PCSK9_ProteinaseK-like.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR PANTHER; PTHR43806:SF59; CEREVISIN-RELATED; 1.
DR PANTHER; PTHR43806; PEPTIDASE S8; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF54897; Protease propeptides/inhibitors; 1.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Protease; Reference proteome; Serine protease.
FT CHAIN 1..467
FT /note="Sexual differentiation process putative subtilase-
FT type proteinase isp6"
FT /id="PRO_0000076410"
FT DOMAIN 86..176
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 186..467
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 221
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 253
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 409
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 467 AA; 49367 MW; A60A0EDF62ADF721 CRC64;
MRIPYSNLFS AAAGLALFAS TACAAPVMPA TDSDIAHAGI RPELDNAFYD SHGEAATPKH
KPHAGPNAAP LLSASNADTT GLDSHYIIVL QPDLSEQEFQ AHTNWVSEMH QMDIASQEDE
YYDTSDSNYM FGLKHVYDFG EDSFKGYSGQ FSSNIVEQIR LHPHVIAVER DQVVSIKKLE
TQSGAPWGLA RISHKSVKYD DIGKYVYDSS AGDNITAYVV DTGVSIHHVE FEGRASWGAT
IPSGDVDEDN NGHGTHVAGT IASRAYGVAK KAEIVAVKVL RSSGSGTMAD VIAGVEWTVR
HHKSSGKKTS VGNMSLGGGN SFVLDMAVDS AVTNGVIYAV AAGNEYDDAC YSSPAASKKA
ITVGASTIND QMAYFSNYGS CVDIFAPGLN ILSTWIGSNT STNTISGTSM ATPHVAGLSA
YYLGLHPAAS ASEVKDAIIK MGIHDVLLSI PVGSSTINLL AFNGAQE
//
