ID   J4TUY9_9FIRM            Unreviewed;       778 AA.
AC   J4TUY9;
DT   31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   31-OCT-2012, sequence version 1.
DT   27-MAR-2024, entry version 37.
DE   RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE            EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN   ORFNames=HMPREF1147_1457 {ECO:0000313|EMBL:EJP29879.1};
OS   Selenomonas sp. FOBRC9.
OC   Bacteria; Bacillota; Negativicutes; Selenomonadales; Selenomonadaceae;
OC   Selenomonas.
OX   NCBI_TaxID=936573 {ECO:0000313|EMBL:EJP29879.1, ECO:0000313|Proteomes:UP000006970};
RN   [1] {ECO:0000313|EMBL:EJP29879.1, ECO:0000313|Proteomes:UP000006970}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FOBRC9 {ECO:0000313|EMBL:EJP29879.1,
RC   ECO:0000313|Proteomes:UP000006970};
RA   Durkin A.S., McCorrison J., Torralba M., Gillis M., Methe B., Sutton G.,
RA   Nelson K.E.;
RL   Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC         fragments with terminal 5'-phosphates, ATP is simultaneously
CC         hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC   -!- SIMILARITY: Belongs to the HsdR family.
CC       {ECO:0000256|ARBA:ARBA00008598}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EJP29879.1}.
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DR   EMBL; ALJR01000041; EJP29879.1; -; Genomic_DNA.
DR   RefSeq; WP_009658745.1; NZ_ALJR01000041.1.
DR   AlphaFoldDB; J4TUY9; -.
DR   PATRIC; fig|936573.3.peg.1967; -.
DR   eggNOG; COG4096; Bacteria.
DR   OrthoDB; 9758243at2; -.
DR   Proteomes; UP000006970; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   CDD; cd18032; DEXHc_RE_I_III_res; 1.
DR   CDD; cd18799; SF2_C_EcoAI-like; 1.
DR   Gene3D; 3.90.1570.30; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR013670; EcoEI_R_C_dom.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR   PANTHER; PTHR47396:SF1; ATP-DEPENDENT HELICASE IRC3-RELATED; 1.
DR   PANTHER; PTHR47396; TYPE I RESTRICTION ENZYME ECOKI R PROTEIN; 1.
DR   Pfam; PF08463; EcoEI_R_C; 1.
DR   Pfam; PF04313; HSDR_N; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT   DOMAIN          179..338
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51192"
FT   REGION          545..587
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   778 AA;  89331 MW;  A2FD0AB81344EAB2 CRC64;
     MDKKSLTEQE IRTRYITPAI SQAGWAPAQI REEYAITRGR IIARGGTCRR EKAKYADYVL
     FFKPHIPLAI VEAKDNQHSV SDGMQQALDY AERLLIPFVF TSNGDGFTFH NCMPGAGDKE
     QLISSEAFPS PEQLWRMYKE GIGLTEEQEA VITEPYYTER EDRSPRYYQR NAINLTVDAV
     VREKKDRIML VMATGTGKTY TAFQIIWRLW KAGVKKRILF LADRNALIDQ TYSNDFSPFK
     DKMTMIRNRK ADKSYEIYLA LYQGLTGEDE KDVFKQFSPT FFDLIIIDEC HRGSAKADSA
     WREILEYFGT ATQIGLTATP KETKDVSNID YFGAPIYIYS LKQGIDDGFL APYRVIRIIL
     DKDAAGWRPY VGQTDRYGNL IEDREYNAVD YDRTLVLEQR TRMVAKVVSN YLKKHDLRFD
     KSIFFCVDTD HADRMRQALI NENSDLVEQE THYVMRITGD DDIGKKQLDN FRDVSARCPV
     LVTTSKLLTT GVDAQMVKFI VLDSNINSMT EFKQIIGRGT RVREDLGKMF FTIFDFRNVT
     KLFADPDFDG PSEQDGEFTP GSEGDIPEFP GDKDDAAQPD GSIPSVPTRE HRVKYYVHNV
     AVEVLGERIQ YIDKDGNLIT ESLTDYTRHN MLHQYATLDA FLNAWSDAER KQVILDELAR
     QGILIEELQE QIGREFDPFD LLCHIVYDKP LRTRAERANH VKKQNYFEKY GAQAQQVLYA
     LLDQYADAGI SDLENMDVLK LKPLSDFGNP AYIVTRIFKG KKDFADALKK LETALYVA
//