ID J7Q9M7_METSZ Unreviewed; 556 AA.
AC J7Q9M7;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE {ECO:0000256|ARBA:ARBA00013280};
GN Name=nifE {ECO:0000313|EMBL:CCJ08077.1};
GN OrderedLocusNames=BN69_2626 {ECO:0000313|EMBL:CCJ08077.1};
OS Methylocystis sp. (strain SC2).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Methylocystaceae; Methylocystis.
OX NCBI_TaxID=187303 {ECO:0000313|EMBL:CCJ08077.1, ECO:0000313|Proteomes:UP000005263};
RN [1] {ECO:0000313|EMBL:CCJ08077.1, ECO:0000313|Proteomes:UP000005263}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC2 {ECO:0000313|EMBL:CCJ08077.1,
RC ECO:0000313|Proteomes:UP000005263};
RX PubMed=23045511; DOI=10.1128/JB.01446-12;
RA Dam B., Dam S., Kube M., Reinhardt R., Liesack W.;
RT "Complete Genome Sequence of Methylocystis sp. Strain SC2, an Aerobic
RT Methanotroph with High-Affinity Methane Oxidation Potential.";
RL J. Bacteriol. 194:6008-6009(2012).
CC -!- FUNCTION: This protein may play a role in the biosynthesis of the
CC prosthetic group of nitrogenase (FeMo cofactor).
CC {ECO:0000256|ARBA:ARBA00003171}.
CC -!- PATHWAY: Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005155}.
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
CC {ECO:0000256|ARBA:ARBA00011002, ECO:0000256|RuleBase:RU004021}.
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DR EMBL; HE956757; CCJ08077.1; -; Genomic_DNA.
DR RefSeq; WP_014892105.1; NC_018485.1.
DR AlphaFoldDB; J7Q9M7; -.
DR STRING; 187303.BN69_2626; -.
DR KEGG; msc:BN69_2626; -.
DR PATRIC; fig|187303.17.peg.2856; -.
DR eggNOG; COG1251; Bacteria.
DR eggNOG; COG2710; Bacteria.
DR HOGENOM; CLU_025876_1_1_5; -.
DR OrthoDB; 9762718at2; -.
DR UniPathway; UPA00782; -.
DR Proteomes; UP000005263; Chromosome.
DR GO; GO:0016163; F:nitrogenase activity; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR CDD; cd01968; Nitrogenase_NifE_I; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.50.12380; Nitrogenase MoFe cofactor biosynthesis protein NifE, C-terminal; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005973; NifE.
DR NCBIfam; TIGR01283; nifE; 1.
DR PANTHER; PTHR42956; NITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN NIFE; 1.
DR PANTHER; PTHR42956:SF1; NITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN NIFE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU004021}.
FT DOMAIN 45..444
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
FT DOMAIN 496..545
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
SQ SEQUENCE 556 AA; 60653 MW; 966611CAF7E596FA CRC64;
MSSLSATIQD VFNEPGCGKN ANKSEAERKK GCTKQLQPGG AAGGCAFDGA KIALQPLTDV
AHLVHGPIAC EGNSWDNRGA KSSGSNLWRT GFTTDINETD VVFGGEKRLF KSIREIIEKY
DPPAVFVYQT CVPAMIGDDI DAVCKAAREK FNKPVIPINS PGFVGPKNLG NKLAGEALLQ
HVIGTEEPEY TTPYDLNIIG EYNLSGELWQ VKPLLDELGI RIMACISGDG KYKEVASSHR
AKAAMMVCSK AMINVARKME ERYGIPFFEG SFYGIEDSSD SLREIARMLI ERGAPAELME
RTEALIAREE AKAWAAIAKY KPRFQGKKVL LITGGVKSWS VVAALQEAGL ELVGTSVKKS
TKEDKERIKE LMGQDAHMIE DMTPREMYKM LKDARADIML SGGRSQFIAL KASMPWLDIN
QERHHAYMGY VGMVKLVEEI DKALYNPVWA QVRKPAPWEK SGDNWQSRAM AQAEAEAAAL
AADPVKAEEV RRAKKICNCK SVELGVIEDA IIASNLSTVE GVRDATNASG GCGACAVRIE
EILERMVSAS HAIAAE
//
