ID   J9VJB1_CRYNH            Unreviewed;      1161 AA.
AC   J9VJB1;
DT   28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT   13-NOV-2013, sequence version 2.
DT   24-JAN-2024, entry version 43.
DE   SubName: Full=Insulysin {ECO:0000313|EMBL:AFR94313.2};
GN   ORFNames=CNAG_07805 {ECO:0000313|EMBL:AFR94313.2};
OS   Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS   CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=235443 {ECO:0000313|EMBL:AFR94313.2, ECO:0000313|Proteomes:UP000010091};
RN   [1] {ECO:0000313|EMBL:AFR94313.2, ECO:0000313|Proteomes:UP000010091}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487
RC   {ECO:0000313|Proteomes:UP000010091};
RX   PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA   Janbon G., Ormerod K.L., Paulet D., Byrnes E.J.III., Yadav V.,
RA   Chatterjee G., Mullapudi N., Hon C.C., Billmyre R.B., Brunel F., Bahn Y.S.,
RA   Chen W., Chen Y., Chow E.W., Coppee J.Y., Floyd-Averette A., Gaillardin C.,
RA   Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L.,
RA   Hsueh Y.P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W.,
RA   Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C.,
RA   Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q.,
RA   Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA   Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA   Cuomo C.A., Dietrich F.S.;
RT   "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT   grubii reveals complex RNA expression and microevolution leading to
RT   virulence attenuation.";
RL   PLoS Genet. 10:E1004261-E1004261(2014).
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
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DR   EMBL; CP003823; AFR94313.2; -; Genomic_DNA.
DR   RefSeq; XP_012048474.1; XM_012193084.1.
DR   AlphaFoldDB; J9VJB1; -.
DR   GeneID; 23890622; -.
DR   KEGG; cng:CNAG_07805; -.
DR   VEuPathDB; FungiDB:CNAG_07805; -.
DR   HOGENOM; CLU_004639_1_2_1; -.
DR   OrthoDB; 129328at2759; -.
DR   Proteomes; UP000010091; Chromosome 4.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   InterPro; IPR032632; Peptidase_M16_M.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   Pfam; PF16187; Peptidase_M16_M; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          131..267
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          295..481
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          487..774
FT                   /note="Peptidase M16 middle/third"
FT                   /evidence="ECO:0000259|Pfam:PF16187"
FT   DOMAIN          778..959
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   1161 AA;  131408 MW;  EE2DAEED92D006D8 CRC64;
     MLRSFASSAG ASTHLIRTHH SLHRLVTPRS RSLALASGRH LTTTLVKPTT KPQSFQSHRL
     SNTARSLFKR SFATPVSRQI SPIKLPIEPI MESPYPPCPP IPKANGPVDL ILPPTEDRKH
     KYFTLSNGLE VVVVSDPKAD KAAASMDVGV GHLSDPDDLP GCAHFCEHLL FMGTKTHPSE
     NAYQQYLSSH NGHSNAWTAM TSTNYYFDVS PDALKGALDR FSGFFSEPLF NEDCTEREIK
     AVDSEHKKNL QNDVWRFYQL EKHLSKPGHP YGKFGTGNYE SLWSVPKEAG RDPRRQLIEW
     WEKEYCARRM KLAVAGKEDV DTLEKWVREK FENVPVRTEG KPEVGRDGVR VVFDESPYGK
     EQLGYFTFTK PVRDMRALEL MFPFPDMDHL YKTRPTHFIS HFLGHEGRGS ILSYLKKKGW
     VNSLSAGNYH DAAGFSLFKI SVDLTPDGLE HYQDVSLTVF KYISLLRSQP PSVDAFNEIK
     AIADISFRFA ERGRTSSYCT NLSSWLQSPV PREKIVSSKW LVEEYNRQEL EWALQLLDPR
     RADIGVTSKV LPKNVVGEYE NKEPIYGTEY KRVKFDEEFL KEAISGAPIA DLQLPGPNLF
     IPEKLDVRKF DVQEPAKRPV ILKDTSLSRL WYKRDDRFWL PKANLDVMLH SPILNVTPRN
     AVLSRLFCDL FSDSITEDVY DADLAELSFN LWNTSHWIQI SAGGFSDKLA VLTEKMLEKF
     VNYKVDEARF QEVAEATRLH WKNFGMSDPW KIGRFYNSYA TQEIAWTQEE KLKELEYITA
     ADVQAFGKEL LTRLHIETLI HGNTSPEGAK EIQDMLERVL KPRELTPTEL KAPRSLVLPS
     SSEYVWQIPV PNESEVNGSV IYEIHVGDPS DITLRNHLSL FSQIAAEPCF DILRTKQQLG
     YIVSGHASQT TGTMGYTVLV QSERDPVYVE TRIEAFLDGL KEIIEEMSEE EFGKHKQSLI
     SKKEEKPKNL GEETKRFWGR IQDRYFEFSR RENDVAELRK TTKQDILNVL MTYIHTSSPT
     RAKLSVHVKS QYKGIKFDLA SAAPLVKSFT EAGIAVDPAA IQKLLSNNPT LEQVKEFANS
     TIDAAANVAD DVKVQLKDVV AELKGQEAGP GAEAGAGPGA EDVKVRPGNV WIEDIQEFKA
     RLVPSKAAVP VEPLKTLVAE L
//