ID J9VRL7_CRYNH Unreviewed; 646 AA.
AC J9VRL7;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 2.
DT 27-MAR-2024, entry version 43.
DE SubName: Full=Transketolase {ECO:0000313|EMBL:AFR95264.2};
GN ORFNames=CNAG_00866 {ECO:0000313|EMBL:AFR95264.2};
OS Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=235443 {ECO:0000313|EMBL:AFR95264.2, ECO:0000313|Proteomes:UP000010091};
RN [1] {ECO:0000313|EMBL:AFR95264.2, ECO:0000313|Proteomes:UP000010091}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487
RC {ECO:0000313|Proteomes:UP000010091};
RX PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA Janbon G., Ormerod K.L., Paulet D., Byrnes E.J.III., Yadav V.,
RA Chatterjee G., Mullapudi N., Hon C.C., Billmyre R.B., Brunel F., Bahn Y.S.,
RA Chen W., Chen Y., Chow E.W., Coppee J.Y., Floyd-Averette A., Gaillardin C.,
RA Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L.,
RA Hsueh Y.P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W.,
RA Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C.,
RA Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q.,
RA Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA Cuomo C.A., Dietrich F.S.;
RT "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT grubii reveals complex RNA expression and microevolution leading to
RT virulence attenuation.";
RL PLoS Genet. 10:E1004261-E1004261(2014).
CC -!- COFACTOR:
CC Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC Evidence={ECO:0000256|ARBA:ARBA00001941};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC Evidence={ECO:0000256|ARBA:ARBA00001964};
CC -!- SIMILARITY: Belongs to the transketolase family.
CC {ECO:0000256|ARBA:ARBA00007131}.
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DR EMBL; CP003824; AFR95264.2; -; Genomic_DNA.
DR RefSeq; XP_012049147.1; XM_012193757.1.
DR AlphaFoldDB; J9VRL7; -.
DR GeneID; 23884640; -.
DR KEGG; cng:CNAG_00866; -.
DR VEuPathDB; FungiDB:CNAG_00866; -.
DR OrthoDB; 1691772at2759; -.
DR Proteomes; UP000010091; Chromosome 5.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006163; P:purine nucleotide metabolic process; IEA:UniProt.
DR Gene3D; 3.40.50.920; -; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR009014; Transketo_C/PFOR_II.
DR InterPro; IPR005475; Transketolase-like_Pyr-bd.
DR InterPro; IPR033248; Transketolase_C.
DR InterPro; IPR005474; Transketolase_N.
DR PANTHER; PTHR43825; PYRUVATE DEHYDROGENASE E1 COMPONENT; 1.
DR PANTHER; PTHR43825:SF1; TRANSKETOLASE-LIKE PYRIMIDINE-BINDING DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF02779; Transket_pyr; 1.
DR Pfam; PF02780; Transketolase_C; 1.
DR Pfam; PF00456; Transketolase_N; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR SUPFAM; SSF52922; TK C-terminal domain-like; 1.
PE 3: Inferred from homology;
FT DOMAIN 66..265
FT /note="Transketolase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00456"
FT DOMAIN 335..438
FT /note="Transketolase-like pyrimidine-binding"
FT /evidence="ECO:0000259|Pfam:PF02779"
FT DOMAIN 519..604
FT /note="Transketolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02780"
SQ SEQUENCE 646 AA; 70864 MW; 1B2D8E2187AEBBED CRC64;
MSVNKVQDWD FEKFPIDLKK YKPFPLDPTK DKKLSQEQKD GLIANISLLR DVIVFFTATG
AARGLAGHTG GAFDTIPEVV ILLSFLLADQ DKSKYVDILF DEAGHRVATQ YLLSVLDGHI
PVEHLLHYRE ANSKLPGHPE LGLTPGVKFS SGRLGHMWPL ANGVALAEKN KAVFMLGSDG
SQQEGDDAEA ARLAVAQGLN VKLFVDDNDV TIAGHPSEYL KGYSVAKTLE GHGLKVVEAN
GEDLDSLYSA IVEIMNHKGP AAVVTHRPMA PKIKGIEGSP HAHDAIKVEP AIEYLDARHP
KCAAILRAIQ PSNYAELLSG STKERGACRV QFGEAVSAVL DKSSKEQNKA KVLVIDSDLE
GSTGLSVIHK KHPEVFLSSG IMERGNFSAA AGWGAFNADR QGVFSTFSAF SEMIISELTM
ARLNFANVLT HFSHSGVDEM ADNTCHFGIN QFFLDNGLED GYDTRLYFPA DCSQMDAIID
RVFYDKGLRF VFSTRSKVPW ILKEDGSKFF DSDYKFVPGK DEVIRKGTRG YVVAYGEILY
RALDAVDRLR KEGLDIGLIN KSTLNVVDED IIKEIGSTEF VFVAESLNRK TGLGSKFGTW
LLERDLRPRY NHIGTSKEGC GGLGEQIGHQ NLGSSDIALK VKQLIK
//
