ID J9WPU6_LEPSV Unreviewed; 810 AA.
AC J9WPU6;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE SubName: Full=Respiratory burst oxidase protein B {ECO:0000313|EMBL:AFS18255.1};
DE EC=1.6.3.1 {ECO:0000313|EMBL:AFS18255.1};
DE Flags: Fragment;
OS Lepidium sativum (Garden cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Lepidieae; Lepidium.
OX NCBI_TaxID=33125 {ECO:0000313|EMBL:AFS18255.1};
RN [1] {ECO:0000313|EMBL:AFS18255.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23095998; DOI=10.1093/jxb/ers284;
RA Muller K., Linkies A., Leubner-Metzger G., Kermode A.R.;
RT "Role of a respiratory burst oxidase of Lepidium sativum (cress) seedlings
RT in root development and auxin signalling.";
RL J. Exp. Bot. 63:6325-6334(2012).
RN [2] {ECO:0000313|EMBL:AFS18255.1}
RP NUCLEOTIDE SEQUENCE.
RA Pang Q.X., Liu X.M., Sun H.H., Zhang S.C., Song X.H., Zhang X.F., Zhang M.,
RA Bai Y., Gao L.L., Zhao B.S.;
RL Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the RBOH (TC 5.B.1.3) family.
CC {ECO:0000256|ARBA:ARBA00007975}.
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DR EMBL; JX312067; AFS18255.1; -; mRNA.
DR AlphaFoldDB; J9WPU6; -.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0016174; F:NAD(P)H oxidase H2O2-forming activity; IEA:UniProtKB-EC.
DR GO; GO:0004601; F:peroxidase activity; IEA:UniProtKB-KW.
DR CDD; cd00051; EFh; 1.
DR CDD; cd06186; NOX_Duox_like_FAD_NADP; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 3.40.50.80; Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR000778; Cyt_b245_heavy_chain.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR013112; FAD-bd_8.
DR InterPro; IPR017927; FAD-bd_FR_type.
DR InterPro; IPR013130; Fe3_Rdtase_TM_dom.
DR InterPro; IPR013121; Fe_red_NAD-bd_6.
DR InterPro; IPR039261; FNR_nucleotide-bd.
DR InterPro; IPR013623; NADPH_Ox.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR11972; NADPH OXIDASE; 1.
DR PANTHER; PTHR11972:SF64; RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN B; 1.
DR Pfam; PF08022; FAD_binding_8; 1.
DR Pfam; PF01794; Ferric_reduct; 1.
DR Pfam; PF08030; NAD_binding_6; 1.
DR Pfam; PF08414; NADPH_Ox; 1.
DR PRINTS; PR00466; GP91PHOX.
DR SFLD; SFLDG01169; NADPH_oxidase_subgroup_(NOX); 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF52343; Ferredoxin reductase-like, C-terminal NADP-linked domain; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS51384; FAD_FR; 1.
PE 2: Evidence at transcript level;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:AFS18255.1}; Peroxidase {ECO:0000256|ARBA:ARBA00022559};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 281..300
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 366..384
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 422..443
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 463..483
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 495..512
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 639..658
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 153..188
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 517..635
FT /note="FAD-binding FR-type"
FT /evidence="ECO:0000259|PROSITE:PS51384"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AFS18255.1"
FT NON_TER 810
FT /evidence="ECO:0000313|EMBL:AFS18255.1"
SQ SEQUENCE 810 AA; 92031 MW; E7B76B8A1BF42E9B CRC64;
RCKATGSHNS EEDCVEITLE ARDESISTMK SRAALRSVVS GRLKTMVKSM SFASRKLDRS
KSSGAIFALR GLRFTAKNDA VGRGWDEVAK RLDELAIGGK LPKSKFGRCI GMAESSEFVN
ELYEALVRRR GTTSSSITKT ELSEFWEQIT GNSFDARLQI FFDMVDKNLD GRITGDEVKE
IIALSASANK LSKIKENVDE YVALIMEELD PDNLGYIELH NLETLLLQVP SQSNDNSPSS
ANKRALNKML SQKLIPTKDR NPVKRYARNV SYFLLENWQR IWVLTLWLSI CVALFTWKFI
QYRRRTVFEV LGYCVSVAKG SAETLKFNMA LILLPVCRNT ITWLRTNSKI VGSVVPFDDN
INFHKVIAFG IAVGVILHSI SHLACDFPRL LHAKNVAYEP IKRFFGEERP DNYGWFMKGI
DGWTGVIMVV LMLVAYVLAQ SWFGRNRANL PKSVKRLIGF NAFWYSHHLF VIVYVLLIVH
GYFIYLSKEW YHKTTWMYLA VPVLLYACER LIRAFRPGSK AVRVLKVAVY PGNVLSLYMS
KPKGFKYTSG QYIYVNCSDV SPFQWHPFSI TSASGDDYLS IHIRTLGDWT SQLKSLFSKV
CQPLSRSQSG LFMANDITRF PRLLIDGPYG APAQDYRNYN VLLLVGLGIG ATPLISIIRD
VLNIIKNQKS IEQNTNNHNV STKSYVATKR AYFYWVTREQ GSLEWFSQVM NEVAEYDSEG
MIELHNYCTS VYEEGDARSA LITMLQSLHH AKSGIDIVSG TRVRTHFARP DWRSVFKHVA
VNHVNQRVGV FYCGNACLIA ELKRLAQDFS
//
