ID K0KI41_WICCF Unreviewed; 322 AA.
AC K0KI41;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Aldo-keto reductase family 1 member {ECO:0000313|EMBL:CCH42681.1};
DE EC=1.1.1.149 {ECO:0000313|EMBL:CCH42681.1};
GN ORFNames=BN7_2225 {ECO:0000313|EMBL:CCH42681.1};
OS Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM
OS 3599 / NBRC 0793 / NRRL Y-1031 F-60-10) (Yeast) (Pichia ciferrii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX NCBI_TaxID=1206466 {ECO:0000313|EMBL:CCH42681.1, ECO:0000313|Proteomes:UP000009328};
RN [1] {ECO:0000313|EMBL:CCH42681.1, ECO:0000313|Proteomes:UP000009328}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL
RC Y-1031 F-60-10 {ECO:0000313|Proteomes:UP000009328};
RX PubMed=23193139; DOI=10.1128/EC.00258-12;
RA Schneider J., Andrea H., Blom J., Jaenicke S., Ruckert C., Schorsch C.,
RA Szczepanowski R., Farwick M., Goesmann A., Puhler A., Schaffer S.,
RA Tauch A., Kohler T., Brinkrolf K.;
RT "Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.";
RL Eukaryot. Cell 11:1582-1583(2012).
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCH42681.1}.
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DR EMBL; CAIF01000050; CCH42681.1; -; Genomic_DNA.
DR AlphaFoldDB; K0KI41; -.
DR STRING; 1206466.K0KI41; -.
DR eggNOG; KOG1577; Eukaryota.
DR HOGENOM; CLU_023205_0_0_1; -.
DR InParanoid; K0KI41; -.
DR Proteomes; UP000009328; Unassembled WGS sequence.
DR GO; GO:0047006; F:17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR11732; ALDO/KETO REDUCTASE; 1.
DR PANTHER; PTHR11732:SF507; ALDO_KET_RED DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 1.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
PE 4: Predicted;
KW Oxidoreductase {ECO:0000313|EMBL:CCH42681.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000009328}.
FT DOMAIN 23..292
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 58
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 118
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 87
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 322 AA; 36282 MW; 8CE3EC5057397E1E CRC64;
MPGQVLATSY KVKLNDGNEI PALGLGGAAP ESDHERFKDA LKAAIRSGIR HIDTAWYYNT
EHIIGAALKE LIDAGEIKRE DLFITTKVWP SLWDKAEYSL DQSLKDLQTD YVDLFLQHWP
ITYRKSETGI PPNPTDSNGV LQFDTEADYL DTYKQLIKLR DNTKKVKSIG VSNFTEKHLQ
RAIDETGVVP AVEQVELHPR LPQKKLVEFA ESKGIKVEAF SPAGSSGAPL IKEPLVVKLS
EKYEVSPNSV LNSYHILSGR IVLPRSSNAE RVKTFIELAP LTEDELKELD QLGIDNPKRF
INDDWGVPLG FEHWKTKFSW ES
//
