ID K0KMQ8_WICCF Unreviewed; 1061 AA.
AC K0KMQ8;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=valine--tRNA ligase {ECO:0000256|ARBA:ARBA00013169};
DE EC=6.1.1.9 {ECO:0000256|ARBA:ARBA00013169};
DE AltName: Full=Valyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00029936};
GN Name=VAS1 {ECO:0000313|EMBL:CCH42403.1};
GN ORFNames=BN7_1948 {ECO:0000313|EMBL:CCH42403.1};
OS Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM
OS 3599 / NBRC 0793 / NRRL Y-1031 F-60-10) (Yeast) (Pichia ciferrii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX NCBI_TaxID=1206466 {ECO:0000313|EMBL:CCH42403.1, ECO:0000313|Proteomes:UP000009328};
RN [1] {ECO:0000313|EMBL:CCH42403.1, ECO:0000313|Proteomes:UP000009328}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL
RC Y-1031 F-60-10 {ECO:0000313|Proteomes:UP000009328};
RX PubMed=23193139; DOI=10.1128/EC.00258-12;
RA Schneider J., Andrea H., Blom J., Jaenicke S., Ruckert C., Schorsch C.,
RA Szczepanowski R., Farwick M., Goesmann A., Puhler A., Schaffer S.,
RA Tauch A., Kohler T., Brinkrolf K.;
RT "Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.";
RL Eukaryot. Cell 11:1582-1583(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-
CC tRNA(Val); Xref=Rhea:RHEA:10704, Rhea:RHEA-COMP:9672, Rhea:RHEA-
CC COMP:9708, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57762,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78537, ChEBI:CHEBI:456215; EC=6.1.1.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001624};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCH42403.1}.
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DR EMBL; CAIF01000043; CCH42403.1; -; Genomic_DNA.
DR AlphaFoldDB; K0KMQ8; -.
DR STRING; 1206466.K0KMQ8; -.
DR eggNOG; KOG0432; Eukaryota.
DR HOGENOM; CLU_001493_0_1_1; -.
DR InParanoid; K0KMQ8; -.
DR Proteomes; UP000009328; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004832; F:valine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006438; P:valyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07962; Anticodon_Ia_Val; 1.
DR CDD; cd00817; ValRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.287.380; Valyl-tRNA synthetase, C-terminal domain; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02004; Val_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033705; Anticodon_Ia_Val.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR010978; tRNA-bd_arm.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR037118; Val-tRNA_synth_C_sf.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR InterPro; IPR002303; Valyl-tRNA_ligase.
DR NCBIfam; TIGR00422; valS; 1.
DR PANTHER; PTHR11946:SF109; VALINE--TRNA LIGASE; 1.
DR PANTHER; PTHR11946; VALYL-TRNA SYNTHETASES; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00986; TRNASYNTHVAL.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF46589; tRNA-binding arm; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000009328}.
FT DOMAIN 116..737
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 782..929
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 997..1059
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 28..76
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1061 AA; 119740 MW; D61F0CFA5FA36F78 CRC64;
MSDKIENPAQ PEAAAAQATQ AAPAEGAPKV KTEKELEKER KKAEKLAKFK AKEAKKAEDK
KKAEANKESK PKKEKKVVEP IPEFVDKTVA GEKKILVNLE DPSLKAYDPK NVESSWYEWW
VKEGLFEPEF AENGEFKPEG VFCIPAPPPN VTGALHIGHA LTVSIQDTLI RYNRMKGKTV
LFLPGFDHAG IATQSVVEKQ LARDGISRHT LGREAFIKKV WEWKEVYHAK IKSQFTRLGA
SYDWNYERFT LDDNLSKSVT EAFVKLHQEG VIYRASRLIN WSVHLNTALS NLEVDNKEIP
GKTLLSVPGY DEKVEFGVLT SFAYPVENSD EKLVVATTRP ETLFGDTGVA VHPEDPRYKH
LHGKFVIHPF LGTKIPIVTD AEAVDMEFGT GAVKITPGHD TNDYNTGKRN NLEIVNILTD
DGVLNENAGE WAGIKRFDAR KKVIEALKEK GLFVEQTENP MTIPICSRSG DVIEPLLKPQ
WWVSQKDMAA EAIKAVRNGD ITITPKSSEA EYFHWLENIQ DWCISRQLWW GHRCPVYFVQ
IEGETNDEND DNYWVSGRTL QEAEEQAAQR FAGKTYTLRQ DEDVLDTWFS SGLWPFSTLG
WPEKTNDLSK FYPWSMLETG WDILFFWVSR MILLGLKMTG EIPFKEVFCH SLVRDAQGRK
MSKSLGNVVD PLDVITGISL ENLHSKLLGG NLAAKEVEKA KAGQKESYPN GIPQCGTDAL
RFALCAYTTG GRDINLDILR VEGYRKFCNK IYQATKFALL RLGDDYKPPA TEGLTGQESL
VEKWILHKLN ETSKIVNESF EKRDFLTSTS AIYEYWYQVC DVYIENSKSL ILEGSEAERK
SARDTLYTTI DGALRLIHPF MPFLSEEMWQ RLPRRGSDNT KSIVKAAYPT YNKEFDNAKA
SADYETILDV TKEARSLLAQ YNIIKNGKVF VEASNAEQFE TITAQKESIV ALIKAIDSIE
VVKSVEEIPQ GVVLQAVSPE INVHLLVKGH VDIDAEISKA QKKLDKVQKS KQSIEKIVNS
KDYASKANQE AQEQNKTKLE NAAAEIEGFE QTIANLERLK L
//
