ID K2Q7K3_9FLAO Unreviewed; 545 AA.
AC K2Q7K3;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=Glycoside hydrolase family protein {ECO:0000313|EMBL:EKF56856.1};
GN ORFNames=I215_01540 {ECO:0000313|EMBL:EKF56856.1};
OS Galbibacter marinus.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Galbibacter.
OX NCBI_TaxID=555500 {ECO:0000313|EMBL:EKF56856.1, ECO:0000313|Proteomes:UP000007364};
RN [1] {ECO:0000313|EMBL:EKF56856.1, ECO:0000313|Proteomes:UP000007364}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ck-I2-15 {ECO:0000313|Proteomes:UP000007364};
RX PubMed=23209227; DOI=10.1128/JB.01852-12;
RA Lai Q., Li C., Shao Z.;
RT "Genome Sequence of Galbibacter marinum Type Strain ck-I2-15.";
RL J. Bacteriol. 194:6973-6973(2012).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKF56856.1}.
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DR EMBL; AMSG01000001; EKF56856.1; -; Genomic_DNA.
DR RefSeq; WP_008990185.1; NZ_AMSG01000001.1.
DR AlphaFoldDB; K2Q7K3; -.
DR STRING; 555500.I215_01540; -.
DR PATRIC; fig|555500.3.peg.323; -.
DR eggNOG; COG3507; Bacteria.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000007364; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd09001; GH43_FsAxh1-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF13; HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00930)-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000007364}.
FT DOMAIN 347..544
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 53
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 219
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 161
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 545 AA; 61794 MW; 27F2EB57E9C12026 CRC64;
MIRIRFYTFL VVSVLCGLLS IVKAQNPYQS IVWQSDQGDG TYKNPVLYAD YSDPDVIRVG
EDYYMTASSF NCAPGLPILH SKDMVNWQLV NHALPFQVPN EHFSVPQHGN GVWAPSIRYH
NEHYYIYWGD PDFGIYMVKT KDPFGSWDPP VLVMEAKGAI DPSPLWDDDG KAYLVHAWAG
SRAGVKSLLT VHRMNAEGTK VLDAGKHVFD GHDEHPTVEG SKFYKRNGYY YIFAPAGGVA
TGWQLILRSK DIYGPYQEKI VLEQGSTTVN GPHQGAWVDT PEGTSWFYHF QDLDPYGRLV
HLQPMQWKDN WPVMGEDYDG NGVGEPVRQY KKPISGADYP IQQPVESDEF NSDELGLQWQ
WQANPNVLWS AQIRGTEHLR LFSIKTDPKD KNLWTAPNLL LQKFPAPEFS ATTKVRLYPE
EATSGKRAGL IIMGMDYAAL SIVYDNQKYY VQRSESINAI DQQQEVVLDK ALLTSNQVYF
RVEVVGPKGQ CQFSYSENGK KFKNIGKPFY AQPGKWIGAK VGLFCVSTPE AKRGGYADFD
WFRVD
//
