ID K4EP11_DATST Unreviewed; 1388 AA.
AC K4EP11;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000256|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000256|HAMAP-Rule:MF_01324,
GN ECO:0000313|EMBL:AEQ36928.1};
GN ORFNames=Dast_Cp012 {ECO:0000313|EMBL:AEQ36928.1};
OS Datura stramonium (Jimsonweed) (Common thornapple).
OG Plastid; Chloroplast {ECO:0000313|EMBL:AEQ36928.1}.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Datureae; Datura.
OX NCBI_TaxID=4076 {ECO:0000313|EMBL:AEQ36928.1};
RN [1] {ECO:0000313|EMBL:AEQ36928.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NN003 {ECO:0000313|EMBL:AEQ36928.1};
RA Li X.W., Li Q., Lin X.H., Hu Z.G., Chen S.L.;
RT "High-throughput multiplex sequencing reveals the feasibility of
RT chloroplast genomes as a plant super-barcode.";
RL Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000256|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01324}.
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DR EMBL; JN662489; AEQ36928.1; -; Genomic_DNA.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR CDD; cd02655; RNAP_beta'_C; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 1.10.1790.20; -; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR NCBIfam; TIGR02388; rpoC2_cyan; 1.
DR PANTHER; PTHR48355; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1.
DR PANTHER; PTHR48355:SF1; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000313|EMBL:AEQ36928.1};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|HAMAP-Rule:MF_01324};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01324};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01324}; Plastid {ECO:0000313|EMBL:AEQ36928.1};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_01324};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01324};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|HAMAP-Rule:MF_01324}.
FT DOMAIN 93..157
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF05000"
FT DOMAIN 172..365
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04998"
FT DOMAIN 1192..1278
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04998"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01324"
FT BINDING 291
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01324"
FT BINDING 298
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1388 AA; 156976 MW; D0C849617DD8487B CRC64;
MAERANLVFH NKAIDGTAMK RLISRLIEHF GMAYTSHILD QVKTLGFQQA TATSISLGID
DLLTIPSKGW LVQDAEQQSL ILEKHHHYGN VHAVEKLRQS IEIWYATSEY LRQEMNPNFR
MTDPFNPVHI MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRRTDC GTARGISVSP RNGMMPERIF
SQTLIGRVLA DDIYMGPRCI ATRNQDIGIG LVNRFITLRA QPISIRTPFT CRSTSWICRL
CYGRSPTHGD LVELGEAVGI IAGQSIGEPG TQLTLRTFHT GGVFTGGTAE HVRAPSNGKI
KFNEDLVHPT RTRHGHPAFL CSIDLYVTIE SEDILHNVNI PPKSLLLVQN DQYVESEQVI
AEIRAGISTL NFKEKVRKHI YSDSDGEMHW STDVYHAPEF TYGNVHLLPK TSHLWILLGG
PCRSSIVYLS IHKDQDQMNS HSLSGKRRYT SNLSVTNDQA RQKLFSSDFY GKKEDRIPDY
SDLNRIICTG QYNLVYSPIL HENSDLLSKR RRNKFIIPLH SIQELENELM PCSGISIQIP
VNGIFRRNSI LAYFDDPRYR RKSSGIIKYG TIETHSVIKK EDLIEYRGVK EFRLKYQMKV
DRFFFIPEEV HILPGSSSIM VRNNSIVGVD TQITLNLRSR VGGLVRVEKK KKRIELKIFS
GDIHFPGETD KISRHTGVLI PPGRGKINSK ESKKVKNWIY VQRITPSKKK FFVLVRPVVT
YEITDGINLA TLFPPDPLQE RDNVQLRIVN YILYGNGKPI RGISDTSIQL VRTCLVLNWN
QDKKSSSCEE ARASFVEIRT NGLIRHFLRI NLVKSPISYI GKRNDPSGSG LLSDNGSDCT
NLNPFSSIYY YSKEKIQQSL NQPQGTIHTL LNRNKECQSL IILSAANCSR MGPFKDVKYH
SVIKESIKKD PLIPIRNSLG PLGTCLPIEN FYSSYHLITH NQILVTNYLQ LDNLKQTFQV
IKFKYYLMDE NGKIFHPDPC RNIILNPFNL NWYFLHHNYC EETSKIISLG QFICENVCIA
KNGSPLKSGQ VILVQVDSIV IRSAKPYLAT PGATVHGHYG ETLYEGDTLV TFIYEKSRSG
DITQGLPKVE QVLEVRSIDS ISMNLEKRVE GWNKCITRIL GIPWGFLIGA ELTIAQSRIS
LVNKIQQVYR SQGVQIHNRH IEIIVRQITS KVLVSEDGMS NVFSPGELIG LLRAERMGRA
LEEAICYQVV LLGITRASLN TQSFISEASF QETARVLAKA ALRGRIDWLK GLKENVVLGG
VIPVGTGFKG LVYPSKQHNN IPLETKKKNL FEGEMRDILF HHRKLFDSCL SKKFHDIPEQ
SFIGFNDS
//
