ID K4ES51_9ROSA Unreviewed; 331 AA.
AC K4ES51;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|HAMAP-Rule:MF_00059};
DE Short=PEP {ECO:0000256|HAMAP-Rule:MF_00059};
DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_00059};
DE AltName: Full=Plastid-encoded RNA polymerase subunit alpha {ECO:0000256|HAMAP-Rule:MF_00059};
DE Short=RNA polymerase subunit alpha {ECO:0000256|HAMAP-Rule:MF_00059};
GN Name=rpoA {ECO:0000256|HAMAP-Rule:MF_00059,
GN ECO:0000313|EMBL:AET62687.1};
OS Fragaria chiloensis.
OG Plastid; Chloroplast {ECO:0000313|EMBL:AET62687.1}.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC Fragaria.
OX NCBI_TaxID=101007 {ECO:0000313|EMBL:AET62687.1};
RN [1] {ECO:0000313|EMBL:AET62687.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GP33 {ECO:0000313|EMBL:AET62687.1};
RA Ashman T.-L., Govindarajulu R., Liston A., Parks M., Salamone I.M.;
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AET62687.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GP33 {ECO:0000313|EMBL:AET62687.1};
RX PubMed=23579477; DOI=10.3732/ajb.1200624;
RA Salamone I., Govindarajulu R., Falk S., Parks M., Liston A., Ashman T.L.;
RT "Bioclimatic, ecological, and phenotypic intermediacy and high genetic
RT admixture in a natural hybrid of octoploid strawberries.";
RL Am. J. Bot. 100:939-950(2013).
RN [3] {ECO:0000313|EMBL:AKI30855.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=SAL3 {ECO:0000313|EMBL:AKI30855.1};
RX PubMed=25878088; DOI=10.3732/ajb.1500026;
RA Govindarajulu R., Parks M., Tennessen J.A., Liston A., Ashman T.L.;
RT "Comparison of nuclear, plastid, and mitochondrial phylogenies and the
RT origin of wild octoploid strawberry species.";
RL Am. J. Bot. 102:544-554(2015).
RN [4] {ECO:0000313|EMBL:AKI30855.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=SAL3 {ECO:0000313|EMBL:AKI30855.1};
RA Govindarajulu r., Parks M., Tennessen J.A., Liston A., Ashman T.-L.;
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|ARBA:ARBA00004026, ECO:0000256|HAMAP-Rule:MF_00059}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|HAMAP-Rule:MF_00059};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000256|HAMAP-Rule:MF_00059}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP-
CC Rule:MF_00059}.
CC -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC transcription, whereas the C-terminal domain is involved in interaction
CC with transcriptional regulators and with upstream promoter elements.
CC {ECO:0000256|HAMAP-Rule:MF_00059}.
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000256|ARBA:ARBA00007123, ECO:0000256|HAMAP-Rule:MF_00059}.
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DR EMBL; JN884816; AET62687.1; -; Genomic_DNA.
DR EMBL; KP718884; AKI30855.1; -; Genomic_DNA.
DR RefSeq; YP_007025415.1; NC_019601.1.
DR AlphaFoldDB; K4ES51; -.
DR GeneID; 14210390; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR CDD; cd06928; RNAP_alpha_NTD; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR NCBIfam; TIGR02027; rpoA; 1.
DR PANTHER; PTHR32108; DNA-DIRECTED RNA POLYMERASE SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32108:SF0; DNA-DIRECTED RNA POLYMERASE SUBUNIT ALPHA; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000313|EMBL:AET62687.1};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|HAMAP-Rule:MF_00059};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00059}; Plastid {ECO:0000313|EMBL:AET62687.1};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00059};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00059}.
FT DOMAIN 29..232
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
FT REGION 1..232
FT /note="Alpha N-terminal domain (alpha-NTD)"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00059"
FT REGION 266..331
FT /note="Alpha C-terminal domain (alpha-CTD)"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00059"
SQ SEQUENCE 331 AA; 38185 MW; 598CF5C5EBEAC051 CRC64;
MVREKVTVST RTLQWKCVES RADSKRLYYG RFILAPLMKG QADTIGIAMR RALLGEIEGT
CITRAKSEKI PHEYSTFVGI QESVHEILMN LKEIVLRSNL YGTRNASICV KGPGYVTAQD
IILPPSIEIV DNTQHIANLT EPINLCIELQ IERNRGYRIK TPNNFQEGSY PIDAVFMPVR
NANHSIHSYV NGNEKQEILF IEIWTNGSLT PKEALHEASR NLIDLFIPFL HAEEENLHFE
NNQHKVTLPL FTFHGKLANP KKIKKENTLK YIFIDQSELP PRVYNCLKRS NIHTLLDLLN
NSQEDLMKIK HLRIEDVKHI LNILEIKKHF E
//
