ID K5Y1K7_AGABU Unreviewed; 550 AA.
AC K5Y1K7;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Pseudouridine synthase I TruA alpha/beta domain-containing protein {ECO:0000259|Pfam:PF01416};
GN ORFNames=AGABI1DRAFT_118781 {ECO:0000313|EMBL:EKM81690.1};
OS Agaricus bisporus var. burnettii (strain JB137-S8 / ATCC MYA-4627 / FGSC
OS 10392) (White button mushroom).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Agaricineae; Agaricaceae; Agaricus.
OX NCBI_TaxID=597362 {ECO:0000313|EMBL:EKM81690.1, ECO:0000313|Proteomes:UP000008493};
RN [1] {ECO:0000313|Proteomes:UP000008493}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JB137-S8 / ATCC MYA-4627 / FGSC 10392
RC {ECO:0000313|Proteomes:UP000008493};
RX PubMed=23045686; DOI=10.1073/pnas.1206847109;
RA Morin E., Kohler A., Baker A.R., Foulongne-Oriol M., Lombard V., Nagy L.G.,
RA Ohm R.A., Patyshakuliyeva A., Brun A., Aerts A.L., Bailey A.M.,
RA Billette C., Coutinho P.M., Deakin G., Doddapaneni H., Floudas D.,
RA Grimwood J., Hilden K., Kuees U., LaButti K.M., Lapidus A., Lindquist E.A.,
RA Lucas S.M., Murat C., Riley R.W., Salamov A.A., Schmutz J., Subramanian V.,
RA Woesten H.A.B., Xu J., Eastwood D.C., Foster G.D., Sonnenberg A.S.,
RA Cullen D., de Vries R.P., Lundell T., Hibbett D.S., Henrissat B.,
RA Burton K.S., Kerrigan R.W., Challen M.P., Grigoriev I.V., Martin F.;
RT "Genome sequence of the button mushroom Agaricus bisporus reveals
RT mechanisms governing adaptation to a humic-rich ecological niche.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:17501-17506(2012).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR EMBL; JH971387; EKM81690.1; -; Genomic_DNA.
DR RefSeq; XP_007327545.1; XM_007327483.1.
DR AlphaFoldDB; K5Y1K7; -.
DR STRING; 597362.K5Y1K7; -.
DR GeneID; 18825489; -.
DR KEGG; abp:AGABI1DRAFT118781; -.
DR eggNOG; KOG2553; Eukaryota.
DR HOGENOM; CLU_021971_0_2_1; -.
DR InParanoid; K5Y1K7; -.
DR OMA; NKAFDCR; -.
DR OrthoDB; 12038at2759; -.
DR Proteomes; UP000008493; Unassembled WGS sequence.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR HAMAP; MF_00171; TruA; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000008493};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 294..399
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 1..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 506..550
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..34
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 506..522
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 535..550
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 164
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 223
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 550 AA; 62353 MW; D587C870D8C6A03A CRC64;
MVNPEADLDD RPFKRRRMSL EADLEQKDIM EALDTDGAQA ETVMEEPSTQ ELHSKNKGKG
KDTKKKGRRD RRATSYHKEN KGGESTWEGT ETPEGPKLPR LPKRQCALLI GFCGSGYNGM
QMYPPPLKTI EGTLFKALVE AGAVSQDNAN DPVKVALARA ARTDAGVHAA GNIVSLKMIM
HVPGIPDLVG RVNELLPPEI RLWGYVRAQN SFNARLACDS RKYTYYFPSY LLIPPKPGSN
LHRVLAQHAE SLGHNGSILS HSFWDNPNSS KEDDMRRKRA WRVTQDRIAD LRTAAQKFIG
THNFHNFTVG RDASDKSNMR HMKSIEIADP VTHGDTEWVS VLFHGQSFML HQVRKMMSSL
VLSCRTNTSP NIVTELYKNP DAFIPKMPSL GLLLEEPIFA SYNSRMNTVN EKHKPDSPDY
RPMIDFEPYR SQINEFKDQF IYKNMREIED RDGLFDAWIR MVDNYAGNDL LYLNPEGIIP
DVAIIKKGDK RENPFRERRV FDATSFSEGD HIKTKLQQAE DGRGEDEDGE DGDSDREAKL
DKKQLAETEG
//
