ID K6U9Y4_9CLOT Unreviewed; 407 AA.
AC K6U9Y4;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=Poly-beta-1,6-N-acetyl-D-glucosamine synthase {ECO:0000256|ARBA:ARBA00017381, ECO:0000256|RuleBase:RU364028};
DE Short=Poly-beta-1,6-GlcNAc synthase {ECO:0000256|RuleBase:RU364028};
DE EC=2.4.1.- {ECO:0000256|RuleBase:RU364028};
GN ORFNames=A370_03186 {ECO:0000313|EMBL:EKQ54364.1};
OS Clostridium sp. Maddingley MBC34-26.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1196322 {ECO:0000313|EMBL:EKQ54364.1, ECO:0000313|Proteomes:UP000001325};
RN [1] {ECO:0000313|EMBL:EKQ54364.1, ECO:0000313|Proteomes:UP000001325}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Maddingley MBC34-26 {ECO:0000313|Proteomes:UP000001325};
RX PubMed=23405323;
RA Rosewarne C.P., Greenfield P., Li D., Tran-Dinh N., Bradbury M.I.,
RA Midgley D.J., Hendry P.;
RT "Draft Genome Sequence of Clostridium sp. Maddingley, Isolated from Coal-
RT Seam Gas Formation Water.";
RL Genome Announc. 1:E00081-12(2013).
CC -!- FUNCTION: N-acetylglucosaminyltransferase that catalyzes the
CC polymerization of single monomer units of UDP-N-acetylglucosamine to
CC produce the linear homomer poly-beta-1,6-N-acetyl-D-glucosamine (PNAG,
CC also referred to as PIA), a biofilm adhesin polysaccharide. Requires
CC IcaD for full activity. {ECO:0000256|ARBA:ARBA00024738}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU364028};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU364028}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family.
CC {ECO:0000256|ARBA:ARBA00006739, ECO:0000256|RuleBase:RU364028}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKQ54364.1}.
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DR EMBL; ALXI01000102; EKQ54364.1; -; Genomic_DNA.
DR AlphaFoldDB; K6U9Y4; -.
DR STRING; 1196322.A370_03186; -.
DR PATRIC; fig|1196322.3.peg.3100; -.
DR eggNOG; COG1215; Bacteria.
DR Proteomes; UP000001325; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008375; F:acetylglucosaminyltransferase activity; IEA:InterPro.
DR GO; GO:0043708; P:cell adhesion involved in biofilm formation; IEA:InterPro.
DR CDD; cd06423; CESA_like; 1.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR023853; PGA_PgaC/IcaA.
DR NCBIfam; TIGR03937; PgaC_IcaA; 1.
DR PANTHER; PTHR43630; POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE; 1.
DR PANTHER; PTHR43630:SF1; POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE; 1.
DR Pfam; PF00535; Glycos_transf_2; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU364028};
KW Glycosyltransferase {ECO:0000256|RuleBase:RU364028};
KW Membrane {ECO:0000256|RuleBase:RU364028};
KW Reference proteome {ECO:0000313|Proteomes:UP000001325};
KW Transferase {ECO:0000256|RuleBase:RU364028};
KW Transmembrane {ECO:0000256|RuleBase:RU364028};
KW Transmembrane helix {ECO:0000256|RuleBase:RU364028}.
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU364028"
FT TRANSMEM 301..325
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU364028"
FT TRANSMEM 331..352
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU364028"
FT TRANSMEM 364..386
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU364028"
FT DOMAIN 49..218
FT /note="Glycosyltransferase 2-like"
FT /evidence="ECO:0000259|Pfam:PF00535"
SQ SEQUENCE 407 AA; 47615 MW; 5806732AB1068928 CRC64;
MSKLIAYASS FVFWYPLIMS TVWIIGGVLF YYRREKKPGL PLNESPMVSI LVPCHNEENT
IENTIKRLND LNYPNYEIIA INDGSKDKTE EILNNLSEEY EKLRVIQLKT NAGKANALYL
GLVASKGEFL AGIDADAYLD SDALNYMIPH FITPNYGERV GAVTGNPRVR NRSSLLAKIQ
LCESSSIISL IKRTQRLLGK VMTVSGVVVV FRKRALIDCG LWDRDLITED IGVTWKLQKR
FWDIRYEPKA LCWMLVPETV VGLWKQRVRW AQGGLEVIMR HWNIFLSWKY RRLMPVYIEQ
IFSIFWSITW FILFITMIIQ ITIGMQTFSS IFWQGQYLSF ICLIQFIIAM ALDKQYDEDL
LKYYLWAIWY PTFFWYFSAL VILRAIPKAL FRNRKKFATW DSPDRGL
//
