ID   K6V4W0_9ACTN            Unreviewed;       527 AA.
AC   K6V4W0;
DT   09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT   09-JAN-2013, sequence version 1.
DT   24-JAN-2024, entry version 35.
DE   SubName: Full=Putative FAD-linked oxidase {ECO:0000313|EMBL:GAB91223.1};
GN   ORFNames=GORHZ_125_01070 {ECO:0000313|EMBL:GAB91223.1};
OS   Gordonia rhizosphera NBRC 16068.
OC   Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Gordoniaceae;
OC   Gordonia.
OX   NCBI_TaxID=1108045 {ECO:0000313|EMBL:GAB91223.1, ECO:0000313|Proteomes:UP000008363};
RN   [1] {ECO:0000313|EMBL:GAB91223.1, ECO:0000313|Proteomes:UP000008363}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 16068 {ECO:0000313|EMBL:GAB91223.1,
RC   ECO:0000313|Proteomes:UP000008363};
RA   Takarada H., Isaki S., Hosoyama A., Tsuchikane K., Katsumata H., Baba S.,
RA   Ohji S., Yamazaki S., Fujita N.;
RT   "Whole genome shotgun sequence of Gordonia rhizosphera NBRC 16068.";
RL   Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|PIRSR:PIRSR625650-3};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:GAB91223.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BAHC01000125; GAB91223.1; -; Genomic_DNA.
DR   RefSeq; WP_006334518.1; NZ_BAHC01000125.1.
DR   AlphaFoldDB; K6V4W0; -.
DR   STRING; 1108045.GORHZ_125_01070; -.
DR   eggNOG; COG0277; Bacteria.
DR   OrthoDB; 9811557at2; -.
DR   Proteomes; UP000008363; Unassembled WGS sequence.
DR   GO; GO:0005777; C:peroxisome; IEA:UniProt.
DR   GO; GO:0008609; F:alkylglycerone-phosphate synthase activity; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0008610; P:lipid biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.300.330; -; 1.
DR   Gene3D; 3.30.465.10; -; 1.
DR   InterPro; IPR025650; Alkyl-DHAP_Synthase.
DR   InterPro; IPR004113; FAD-bd_oxidored_4_C.
DR   InterPro; IPR016166; FAD-bd_PCMH.
DR   InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR   InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR   InterPro; IPR016164; FAD-linked_Oxase-like_C.
DR   InterPro; IPR006094; Oxid_FAD_bind_N.
DR   PANTHER; PTHR46568; ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL; 1.
DR   PANTHER; PTHR46568:SF1; ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL; 1.
DR   Pfam; PF02913; FAD-oxidase_C; 1.
DR   Pfam; PF01565; FAD_binding_4; 1.
DR   SUPFAM; SSF56176; FAD-binding/transporter-associated domain-like; 1.
DR   SUPFAM; SSF55103; FAD-linked oxidases, C-terminal domain; 1.
DR   PROSITE; PS51387; FAD_PCMH; 1.
PE   4: Predicted;
KW   FAD {ECO:0000256|PIRSR:PIRSR625650-3};
KW   Flavoprotein {ECO:0000256|PIRSR:PIRSR625650-3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008363}.
FT   DOMAIN          92..271
FT                   /note="FAD-binding PCMH-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51387"
FT   ACT_SITE        447
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR625650-1"
FT   BINDING         124..130
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR625650-3"
FT   BINDING         255..261
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR625650-3"
FT   BINDING         386
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR625650-2"
FT   SITE            306
FT                   /note="Important for enzyme activity"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR625650-4"
SQ   SEQUENCE   527 AA;  56981 MW;  27ECE1C6535500C4 CRC64;
     MSSRSWWGWG TDENALSPAE TDALVNRVAA LIPDHDFTDH PPPAPQSLPV PPPRISVPAT
     LSAVFSDDVR DRLSHARGKA FRDVVRNLHG DISHVPDLVA RPRTERDVID ILDFCSAEAL
     ACIPYGGGSS VVGGVEPRFD GPAVTLDLGR LDRVVEIDDV TRSARIQAGA LGPHIEDQLR
     PAGLTLRHFP QSFEFSSLGG WLATRAGGHF ATLYTHIDDL TESIRAITPA GVSESRRLPS
     SGAGPSPDRL LLGSEGTLGV ITEAWMRLQD RPRFGTTVSV GFDDYHDAVR ATRTVAQSGL
     YPSNCRLLDP AEAFINAGAE SPGGVLILAF ESADHPVGPW IDRAVEIATD HAGHVLSQRD
     RGERDNTGNH ASAAWRSAFI RMPYQRDALA RRSMIVETFE TACTWDHFDA LHIAVTAVAT
     DTVQRICGAG VVTCRFTHVY PDGPASYYSI YAPGRWGSTV AQWDEIKSAV SEAISNNHGT
     ITHHHAVGRD HRSWYDTERP DLFAAALTAA KHVLDPAGIL NPGVLVG
//