ID K6XC79_9MICO Unreviewed; 1860 AA.
AC K6XC79;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=biotin carboxylase {ECO:0000256|ARBA:ARBA00013263};
DE EC=6.3.4.14 {ECO:0000256|ARBA:ARBA00013263};
GN ORFNames=KILIM_037_00280 {ECO:0000313|EMBL:GAB96409.1};
OS Kineosphaera limosa NBRC 100340.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Dermatophilaceae;
OC Kineosphaera.
OX NCBI_TaxID=1184609 {ECO:0000313|EMBL:GAB96409.1, ECO:0000313|Proteomes:UP000008366};
RN [1] {ECO:0000313|EMBL:GAB96409.1, ECO:0000313|Proteomes:UP000008366}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 100340 {ECO:0000313|EMBL:GAB96409.1,
RC ECO:0000313|Proteomes:UP000008366};
RA Yoshida I., Isaki S., Hosoyama A., Tsuchikane K., Katsumata H., Ando Y.,
RA Ohji S., Hamada M., Tamura T., Yamazoe A., Yamazaki S., Fujita N.;
RT "Whole genome shotgun sequence of Kineosphaera limosa NBRC 100340.";
RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=biotin; Xref=ChEBI:CHEBI:57586;
CC Evidence={ECO:0000256|ARBA:ARBA00001953};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAB96409.1}.
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DR EMBL; BAHD01000037; GAB96409.1; -; Genomic_DNA.
DR STRING; 1184609.KILIM_037_00280; -.
DR eggNOG; COG4770; Bacteria.
DR eggNOG; COG4799; Bacteria.
DR Proteomes; UP000008366; Unassembled WGS sequence.
DR GO; GO:0003989; F:acetyl-CoA carboxylase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004075; F:biotin carboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd06850; biotinyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.30.470.20; ATP-grasp fold, B domain; 1.
DR InterPro; IPR034733; AcCoA_carboxyl_beta.
DR InterPro; IPR013537; AcCoA_COase_cen.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR005481; BC-like_N.
DR InterPro; IPR011764; Biotin_carboxylation_dom.
DR InterPro; IPR005482; Biotin_COase_C.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR005479; CbamoylP_synth_lsu-like_ATP-bd.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR011763; COA_CT_C.
DR InterPro; IPR016185; PreATP-grasp_dom_sf.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR18866; CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE; 1.
DR PANTHER; PTHR18866:SF33; METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED; 1.
DR Pfam; PF08326; ACC_central; 1.
DR Pfam; PF02785; Biotin_carb_C; 1.
DR Pfam; PF00289; Biotin_carb_N; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF01039; Carboxyl_trans; 1.
DR Pfam; PF02786; CPSase_L_D2; 1.
DR SMART; SM00878; Biotin_carb_C; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 2.
DR SUPFAM; SSF56059; Glutathione synthetase ATP-binding domain-like; 1.
DR SUPFAM; SSF52440; PreATP-grasp domain; 1.
DR SUPFAM; SSF51246; Rudiment single hybrid motif; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS50979; BC; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR PROSITE; PS50989; COA_CT_CTER; 1.
DR PROSITE; PS00866; CPSASE_1; 1.
DR PROSITE; PS00867; CPSASE_2; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00409}; Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00409}; Reference proteome {ECO:0000313|Proteomes:UP000008366}.
FT DOMAIN 3..461
FT /note="Biotin carboxylation"
FT /evidence="ECO:0000259|PROSITE:PS50979"
FT DOMAIN 134..332
FT /note="ATP-grasp"
FT /evidence="ECO:0000259|PROSITE:PS50975"
FT DOMAIN 586..669
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 1547..1849
FT /note="CoA carboxyltransferase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50989"
FT REGION 673..698
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1860 AA; 199738 MW; BBD94998A1F25B2C CRC64;
MTLPRRIAIV NRGEAAMRLI HAVRDLNARN GGPQRGAAGR IETVALHTEG ERRAMFVREA
DQAYNLGPAA NRPYLDHGLL ERALRETGAD AVWVGWGFVA EDAEFAALCA RLGITFIGPS
PEAMRKLGDK IGSKLIAQEV GVPVAPWSGG GVDTLEAATA AAAQIGYPLM LKATAGGGGR
GIRRVDSDEQ LADAFERTRD EALRAFGSGV VFLERLVTGA RHVEVQVITD GQGTAWAIGV
RDCSVQRRNQ KVIEESASPV LTDEQAQQLR EAAERLAIAV GYSGAGTVEF LYQPQEQLFA
FLEVNTRLQV EHPITEAVTG TDLVGLQIHV AAGGRLTGER PRQIGHAVEA RLNAEDPDRD
FAPAPGRIAL LDLPSGPGIR VDTGVAQGDE IPADFDSMIA KIIAVGADRD EALARLRRAM
AETTVIIEGG ATNKSFVLDL LEAPEVIDGS ADTGWIDRAR AQGRLVSGRH AGVALVAAGI
EAYEAQEALE RTRLLETARG GRPQVQHDVG RAIDLTLRGA PYSVTVLRVG PQRFRVRVAS
GGAATGGPQR TVVADLDRID EHTSRLSIGG LTHRLVTATH GPTQLVEVDG VTHRVSLDEG
GVLRSPAPAL VVATPVAAGE QVSAGTPVLV LESMKMETVL VAPFTARVKE LLVAAGSQVE
TGAPLVRLEP VEEEVEAPVE PQPQAGPEVE LPEGDALPGD PRERATRLIA DLSAALLGYD
VDTRDNGGTL SGYLKTRDAL VAAGRSPLRE ELGMLEAFAD VAELSRNRPL GEDLHLENQV
HSPREHFHTY LHSLDPERGA LPESFRARLR RVLAHYDVEG LERTPELERA VFRVFLAQQR
AAQQIDLVVS LLQRWLAEPP PPPPLDWEAR DLLDRLVLAT QLRHPVVGDL ARSVKFRWFD
QPLVDAERAS ALGGVRAQLA AIARTPAGPE RDGRVDELVA IPEQIVRFLA ERLEDGQVPA
DEPMLAVLIK RHYRDHGLRD LRELACSGRP FAVADYELDH LPRHVITTVG QVGELRPGSP
LVQCLAEQVA ATSVPGAQVA VDLYLGWPDL PEDPQEASDA LARQLTGLDI AHAVRRVSIA
VSPGDGRPVG YFIFRPQPDG TLEEDVLVRG VHPMVGRRLN LWRLRDFDLT RLDAPEDVLL
YRAVARGNES DQRLVALAQV RQVAVVRDES GRVVGVPHAE RAVANCLEAI RRARAEAGAR
GERLDMNYVW VHIWPPIDAD PDQLGALRRK IAPVTAGAGI EEVLVDGSIL APDGTPTPVA
ARFHYQPGSG VVASFEGPPS VRLKPLDDYA RKVVLARRRG LVYPYALHAM IAGRGGTATE
YDLDETGRLA PVDRPYGQNS AGIIVGVIST PTPRYPEGMT RVLLCGDPLK ALGAVSEPEC
RRIIAALDLA DELGVPVEWF SISAGARISM DSGTENMDWV ARALKRIVEF TQAGGEINVV
VAGINVGAQP YWNAEATMLM HTKGILVMTP DSAMVLTGKQ SLDFSGGVSA EDNYGIGGYD
RVMGPNGQAQ YWAPDLASAI RVLMAHYDHT YVAPGEGGPR RVLTSDPSDR DVTLFPHDPA
ISGFATVGDI FSPATNPDRK KAFDIRTVMS ALADQDYPTL ERWAGMADAD TSVVQDAHIG
GIPVCLIGIE SRAVPRRGYP PTDGPDTYTA GTLFPKSSKK TARAINAASG NRPLVVLANL
SGFDGSPESM RNLQLEYGAE IGRSIVNFDG TIVFCVISRY HGGAFVVFSK ALNPQMTVLA
VEGSFASVIG GAPAAAVVFA RDVETRTAQD ERVRDLEAAM AAETDHTQRA ALATELATVR
RDVRSEKLGA VASEFDRVHD IHRAVAVGSV DEVISAAQLR PKIIAALEAG LAAGLEADPA
//
