ID K7FEV1_PELSI Unreviewed; 1644 AA.
AC K7FEV1;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=Rap guanine nucleotide exchange factor 2 {ECO:0000256|ARBA:ARBA00016709};
DE AltName: Full=Cyclic nucleotide ras GEF {ECO:0000256|ARBA:ARBA00031545};
DE AltName: Full=Neural RAP guanine nucleotide exchange protein {ECO:0000256|ARBA:ARBA00032021};
DE AltName: Full=PDZ domain-containing guanine nucleotide exchange factor 1 {ECO:0000256|ARBA:ARBA00030673};
DE AltName: Full=RA-GEF-1 {ECO:0000256|ARBA:ARBA00029925};
DE AltName: Full=Ras/Rap1-associating GEF-1 {ECO:0000256|ARBA:ARBA00031980};
GN Name=RAPGEF2 {ECO:0000313|Ensembl:ENSPSIP00000006561.1};
OS Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Testudinata; Testudines; Cryptodira; Trionychia;
OC Trionychidae; Pelodiscus.
OX NCBI_TaxID=13735 {ECO:0000313|Ensembl:ENSPSIP00000006561.1, ECO:0000313|Proteomes:UP000007267};
RN [1] {ECO:0000313|Proteomes:UP000007267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Daiwa-1 {ECO:0000313|Proteomes:UP000007267};
RG Soft-shell Turtle Genome Consortium;
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000007267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Daiwa-1 {ECO:0000313|Proteomes:UP000007267};
RX PubMed=23624526; DOI=10.1038/ng.2615;
RA Wang Z., Pascual-Anaya J., Zadissa A., Li W., Niimura Y., Huang Z., Li C.,
RA White S., Xiong Z., Fang D., Wang B., Ming Y., Chen Y., Zheng Y.,
RA Kuraku S., Pignatelli M., Herrero J., Beal K., Nozawa M., Li Q., Wang J.,
RA Zhang H., Yu L., Shigenobu S., Wang J., Liu J., Flicek P., Searle S.,
RA Wang J., Kuratani S., Yin Y., Aken B., Zhang G., Irie N.;
RT "The draft genomes of soft-shell turtle and green sea turtle yield insights
RT into the development and evolution of the turtle-specific body plan.";
RL Nat. Genet. 45:701-706(2013).
RN [3] {ECO:0000313|Ensembl:ENSPSIP00000006561.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell junction {ECO:0000256|ARBA:ARBA00004282}.
CC Cell membrane {ECO:0000256|ARBA:ARBA00004236}. Cytoplasm, perinuclear
CC region {ECO:0000256|ARBA:ARBA00004556}. Endosome
CC {ECO:0000256|ARBA:ARBA00004177}. Late endosome
CC {ECO:0000256|ARBA:ARBA00004603}.
CC -!- SIMILARITY: Belongs to the RAPGEF2 family.
CC {ECO:0000256|ARBA:ARBA00010829}.
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DR EMBL; AGCU01077569; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077570; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077571; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077572; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077573; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077574; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077575; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077576; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077577; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01077578; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_006121936.1; XM_006121874.2.
DR STRING; 13735.ENSPSIP00000006561; -.
DR Ensembl; ENSPSIT00000006598.1; ENSPSIP00000006561.1; ENSPSIG00000006063.1.
DR GeneID; 102457114; -.
DR CTD; 9693; -.
DR eggNOG; KOG3542; Eukaryota.
DR GeneTree; ENSGT00940000156418; -.
DR HOGENOM; CLU_002782_0_1_1; -.
DR OMA; SSHMDQM; -.
DR OrthoDB; 5473909at2759; -.
DR TreeFam; TF313184; -.
DR Proteomes; UP000007267; Unassembled WGS sequence.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 2.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd01785; RA_PDZ-GEF1; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 2.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR45161; CYTOSKELETON-ASSOCIATED PROTEIN 4; 1.
DR PANTHER; PTHR45161:SF2; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00100; cNMP; 2.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 2.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 2.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 3: Inferred from homology;
KW Cell junction {ECO:0000256|ARBA:ARBA00022949};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Differentiation {ECO:0000256|ARBA:ARBA00022782};
KW Endosome {ECO:0000256|ARBA:ARBA00022753};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Neurogenesis {ECO:0000256|ARBA:ARBA00022902};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000007267};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}.
FT DOMAIN 37..84
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 290..390
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 422..535
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 540..610
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 761..847
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT DOMAIN 872..1099
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 223..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1155..1194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1244..1306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1367..1403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1445..1531
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1584..1644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..251
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1180..1194
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1251..1306
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1457..1481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1484..1498
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1500..1514
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1584..1606
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1644 AA; 184518 MW; C09CB0703B3F5EF4 CRC64;
MASYVDNSFR QAVMKNPTER SPQDLEIVYS YLHGMEALSN LREHQLRLMC ETVRYERHEA
NEVLYYPDDI GTCWYILLSG SVFIKESMFL PRSSFGKRSA GSFRRGCECI VLEPSEMIVV
DYMDENEEYF QRQASHRQSR RRFRKINQKG ERQTIIDTVD PYPVGKPPLP RGYHTLPADF
TKLHLTDSLH PQVTHVTSSH SGCSITSDSG SSSLSDIYQA TESEAGDMDL SGLPETAVDS
EDDDDEEDIE RASDPLMSRD IVRDCLEKDP IDRTDDDIEQ LLEFMHQLPA FANMTMSVRR
ELCAVMVFAV VERAGTIVLN DGEELDSWSV ILNGSVEVTH PDGRTEILCM GNSFGVSPTM
DKEYMKGVMR TKVDDCQFVC IAQQDYCRIL NQVEKNMQKV EEEGEIVMVK EHRELDRTGT
RKGHIVIKGT SERLTMHLVE EHSVVDPTFI EDFLLTYRTF LSSPMEVGKK LLEWFNDPSL
RDKVTRVVLL WVNNHFNDFE GDPAMTRFLE EFENNLEREK MGGHLRLLNI ACAAKAKRRL
ITLTKPSREA PLPFILLGGS EKGFGIFVDS VDLGSKATEA GLKRGDQILE VNGQNFENIQ
LSKAMEILRN NTHLSITVKT NLFVFKELLT RLSEEKRNGA PHLPKIGDIK KASRYSIPDL
AVDVEQVIGL EKVNKKSKAN TVGGRNKLKK ILDKTRISIL PQKPYNDIGI GQSQDDSIVG
LRQTKHIPPA LPVSGTLSSS NPDLLQSHHR ILDFNTTPDL PDQVLRVFKA DQQSRYIMIS
KDTTAKEVVI QAIREFAVTA TPDAYSLCEV SVTPEGVIKQ RRLPDQLSKL ADRIQLSGRY
YLKNNMETET LCSDEDAQEL LRESQISLLQ LSTIEMAAQL SMRNFELFRN IEPTEYIDDL
FKLKSKTSCA NLKKFEEVIN QETFWVASEI LRETNQLKRM KIIKHFIKIA LHCRECKNFN
SMFAIISGLN LAPVARLRTT WEKLPSKYEK LFQDLQDLFD PSRNMAKYRN VLNSQNLQPP
IIPLFPVIKK DLTFLHEGND SKVEGLVNFE KLRMIAKEIR HVGRMASVNI DPAVMFRTRK
KKWRSLGSLS QGSTNAAVLD VAQTGGHKKR VRRSSFLNAK KLYEDAQMAR KVKQYLSNLD
LEMDEESLQT LSLQCEPATN TLPKNPGDKR SGKQETSPVA PRAGIQQKMQ QQHKINQALQ
VPAVSLYPSR KKVPVKDLPP FGINSPQALK KILSLSEEGI LDRHKKQAED TISNASSQLS
SPPTSPQTSP RKGYTLAPSG TVDNFSDSGH SEISSRSSIV SNSSFDSMPV SLHDERRQRH
SVSIVETNLG VGRIDRRPMI EPDQYSLGSY APLSENRGLY AGATVLSSPS TEELSQDQGD
RTSLDAADSG RGSWTSCSSG SHDNIQTIQH QRSWETLPFG HIHFDSSGDG AGLWASGSHM
DQMMFPDHGT KYGRQSQGRE GLDQAQSRAS WASSTGYWGE DSEGDTGTIK RRGGKDVSME
AETSSITSVT AEESKPAPMP SHIAVSSSSA KGLIARKEGR YREPPPTPPG YVGIPIADFA
EGISHPTRKP PDYNVALQRS RMVARTCETQ GTSTLPQQSH SHSAGKPGNK PQWHKPNESD
PRLAHYPSQG FSTEEDEDEQ VSAV
//
