ID K7M014_SOYBN Unreviewed; 1637 AA.
AC K7M014;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 75.
DE RecName: Full=Dicer-like protein 4 {ECO:0008006|Google:ProtNLM};
GN Name=100797277 {ECO:0000313|EnsemblPlants:KRH20103};
GN ORFNames=GLYMA_13G156500 {ECO:0000313|EMBL:KRH20103.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EnsemblPlants:KRH20103};
RN [1] {ECO:0000313|EMBL:KRH20103.1, ECO:0000313|EnsemblPlants:KRH20103}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH20103};
RC TISSUE=Callus {ECO:0000313|EMBL:KRH20103.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRH20103}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH20103};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRH20103.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRH20103.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SIMILARITY: Belongs to the helicase family. Dicer subfamily.
CC {ECO:0000256|ARBA:ARBA00035116, ECO:0000256|PROSITE-ProRule:PRU00657}.
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DR EMBL; CM000846; KRH20103.1; -; Genomic_DNA.
DR RefSeq; XP_006594227.1; XM_006594164.2.
DR AlphaFoldDB; K7M014; -.
DR SMR; K7M014; -.
DR STRING; 3847.K7M014; -.
DR PaxDb; 3847-GLYMA13G22450-2; -.
DR EnsemblPlants; KRH20103; KRH20103; GLYMA_13G156500.
DR GeneID; 100797277; -.
DR Gramene; KRH20103; KRH20103; GLYMA_13G156500.
DR KEGG; gmx:100797277; -.
DR eggNOG; KOG0701; Eukaryota.
DR InParanoid; K7M014; -.
DR OrthoDB; 342391at2759; -.
DR Proteomes; UP000008827; Chromosome 13.
DR ExpressionAtlas; K7M014; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0030422; P:siRNA processing; IBA:GO_Central.
DR CDD; cd18034; DEXHc_dicer; 1.
DR CDD; cd19869; DSRM_DCL_plant; 1.
DR CDD; cd19875; DSRM_EIF2AK2-like; 1.
DR CDD; cd00593; RIBOc; 2.
DR Gene3D; 3.30.160.20; -; 2.
DR Gene3D; 3.30.160.380; Dicer dimerisation domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 2.170.260.10; paz domain; 1.
DR Gene3D; 1.10.1520.10; Ribonuclease III domain; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR038248; Dicer_dimer_sf.
DR InterPro; IPR005034; Dicer_dimerisation_dom.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR003100; PAZ_dom.
DR InterPro; IPR036085; PAZ_dom_sf.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR036389; RNase_III_sf.
DR PANTHER; PTHR14950:SF15; DICER-LIKE PROTEIN 4; 1.
DR PANTHER; PTHR14950; DICER-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF03368; Dicer_dimer; 1.
DR Pfam; PF14709; DND1_DSRM; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF02170; PAZ; 1.
DR Pfam; PF00636; Ribonuclease_3; 2.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00358; DSRM; 2.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00949; PAZ; 1.
DR SMART; SM00535; RIBOc; 2.
DR SUPFAM; SSF54768; dsRNA-binding domain-like; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF101690; PAZ domain; 1.
DR SUPFAM; SSF69065; RNase III domain-like; 2.
DR PROSITE; PS51327; DICER_DSRBF; 1.
DR PROSITE; PS50137; DS_RBD; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50821; PAZ; 1.
DR PROSITE; PS00517; RNASE_3_1; 1.
DR PROSITE; PS50142; RNASE_3_2; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00657};
KW RNA-mediated gene silencing {ECO:0000256|ARBA:ARBA00023158}.
FT DOMAIN 56..232
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 393..555
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 577..667
FT /note="Dicer dsRNA-binding fold"
FT /evidence="ECO:0000259|PROSITE:PS51327"
FT DOMAIN 872..972
FT /note="PAZ"
FT /evidence="ECO:0000259|PROSITE:PS50821"
FT DOMAIN 1023..1168
FT /note="RNase III"
FT /evidence="ECO:0000259|PROSITE:PS50142"
FT DOMAIN 1209..1353
FT /note="RNase III"
FT /evidence="ECO:0000259|PROSITE:PS50142"
FT DOMAIN 1380..1448
FT /note="DRBM"
FT /evidence="ECO:0000259|PROSITE:PS50137"
FT DOMAIN 1552..1628
FT /note="DRBM"
FT /evidence="ECO:0000259|PROSITE:PS50137"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1637 AA; 183917 MW; D2A548F69764C009 CRC64;
MPDGESSAVA GGQVSMEPSL SLSDQLQSLS LSQVKNHDDS VKKDPRKIAR RYQLELCKKA
MEENIIVYLG TGCGKTHIAV LLMHEMGDLI RKPQKNICVF LAPTVALVHQ QAKVIADSTD
FKVGTYCGSS KRLKHHQDWE QEMGQYEVLV MTPQILHHNL SHCFITMEMI ALLIFDECHH
AQVKSNHAYA VIMKVFYKSN STKVPRIFGM TASPVVGKGA SSEANLAKSI NSLEHILDAK
VYSVEDKELQ SFVTTPVINI YHYVSTASGE TSLHLKIEEI KRQCIATLGR SIEDHQKRMN
TKKLLNRMHD NVIFGLQNLG IWGALQASHI LLSGDHSERH ELVEADGNSS DDSLCDKYLA
QAAELFTSQC MIGDRVTDLS SVEILKEPFF SAKLLRLIGI LSNFRLQKNM KCIIFVNRIV
TARSLSYILQ KLKLLRQWRS DFLVGVHAGL KSMSRKTMNI IVDKFRSGEL NLLVATKVGE
EGLDIQTCCL VIRFDLPETV ASFIQSRGRA RMPQSEYAFL VDSGNKKEID VIDGFKEDEY
RMNMEVTFRT SKETYIIPEE RIFRIDSSGA SVSSGYSISL LHQYCSKLPH DEYFDPKPSF
HYLDDSGGIS CHITLPSNAP INQILGTPQL SMEASKREAC LKAIEELYNL GALSDCLLPK
QDDAEPEVQV SGSSDEDECE DAISRGKLHE MLVPSAFGQS WINEDNIVRL NSYYIKFCPY
PEDRVYKEFG LFMMTCLPME AEKLELDLHL AHGRSVMTMF VPFGVVEFNK DEIKMAENFQ
EMFLKIILDR LEFISEFVDL GMSAESHSGT STFYLLLPVV LQEYGNAMEV DWKIVKRCLC
SPIFRHPADT MDKKVFPLDI HLQLANGYRS VRNVENSLVY APHKKNFYFV TNVNYEKNGY
SPHNDSGTSS YVDYFIEKFS IHLKCPKQPL LHVKPVSNLH NLLHNRKRED AEPQELDEYL
IYLPPELCEL KVIGFSKDIG SSISLLPSIM HRLGNLLVAI ELKHMLSSSF PEAAEISAIR
VLEALTTEKC QERFSLERLE VLGDAFLKFA VARHFFLMHD SLHEGDLTKR RSNAVNNSNL
FKLAIKRNLQ VYICDQTFDP TQFYALGRPC PRVCSNETEE SIHFCLNSVM QQGKATETRC
SKNHHWLHRK TIADVVEALV GAFLVDSGFK AAIAFLSWIG IQVDFEASQV VDICIASASY
SPLSSEVDIP SLEGKLGHHF FHKGLLLQAF VHPSYNKLGG GCYQRLEFLG DAVLDYLITS
YLFSAYPKLK PGQLTDLRSL SVNNKAFACL AVDRSFDNFL LCDSSGLSEA IKKYVDYVRR
PVSDNGIKEG PKCPKALGDL VESCVGAILL DSGFNLNKVW KIMTSFLDPI MKFSSSLQLS
PVRDLRELCQ SHNLELEFLP VPSKLTKRFS VEAKVSGNGV CETASATGQN KKEACRIASQ
LLFLKFKAQG WKAKSKTLEE VLESTSKMEP KLIGYDETPI DVTDTNTAKH IVVNADPYNN
SNPEICPMQL TDEICSPCVK PFGQRLQSSA KGKLSQIFEN RDCGSDSSGT GTARSRLYEL
CAAYCWKPPS FECCKKEGPD HLKQFTCKVT LEIEEAQNLI LEFVGEPLSK KKDAAESAAE
GALWYLQHEG FLPSIGN
//
