ID K9CY90_SPHYA Unreviewed; 755 AA.
AC K9CY90;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=NADP-dependent malic enzyme {ECO:0008006|Google:ProtNLM};
GN ORFNames=HMPREF9718_00669 {ECO:0000313|EMBL:EKU76968.1};
OS Sphingobium yanoikuyae ATCC 51230.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=883163 {ECO:0000313|EMBL:EKU76968.1, ECO:0000313|Proteomes:UP000009887};
RN [1] {ECO:0000313|EMBL:EKU76968.1, ECO:0000313|Proteomes:UP000009887}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51230 {ECO:0000313|EMBL:EKU76968.1,
RC ECO:0000313|Proteomes:UP000009887};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Huys G., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Howarth C., Imamovic A., Larimer J., McCowen C., Montmayeur A.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Sphingobium yanoikuyae ATCC 51230.";
RL Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: In the N-terminal section; belongs to the malic enzymes
CC family. {ECO:0000256|ARBA:ARBA00007686}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKU76968.1}.
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DR EMBL; AGZU01000005; EKU76968.1; -; Genomic_DNA.
DR RefSeq; WP_004207688.1; NZ_JH992904.1.
DR AlphaFoldDB; K9CY90; -.
DR PATRIC; fig|883163.3.peg.676; -.
DR HOGENOM; CLU_012366_0_0_5; -.
DR Proteomes; UP000009887; Unassembled WGS sequence.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10950; -; 1.
DR Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR012188; ME_PTA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR042113; P_AcTrfase_dom1.
DR InterPro; IPR042112; P_AcTrfase_dom2.
DR InterPro; IPR002505; PTA_PTB.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR Pfam; PF01515; PTA_PTB; 1.
DR PIRSF; PIRSF036684; ME_PTA; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036684-2};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|PIRSR:PIRSR036684-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000009887}.
FT DOMAIN 18..151
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 163..399
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
FT ACT_SITE 94
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-1"
FT BINDING 76..83
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 136
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 137
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 162
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 286
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
SQ SEQUENCE 755 AA; 80955 MW; AF26A1D4040B6CC6 CRC64;
MDDNTRRAAL DYHRLPQPGK LAIEPTKRMV NQRDLALAYS PGVAAPCEEI AADPAKALDY
TARGNLVAVI SNGTAVLGLG AIGALASKPV MEGKAVLFKK FADIDVFDIE VDTTDTDKFV
EAVALLEPTF GGINLEDIKA PECFEIERRL KERMNIPVFH DDQHGTAIVV AAAVRNALVL
QGKTLADAKL VTSGAGAAAL ACVDLLVSMG LPIDNVTMTD KDGVIHSGRE GMLPNMARYA
RDTNARTLPD VLPGANLFLG LSAPGVLKPE WLPLMAPNPL IFALANPEPE IRPEAAREVR
PDAIIATGRS DYPNQVNNVL CFPYIFRGAL DCGATQINEA MKVAAADAIA ALARMPAHDS
VAQAYGGRKL VFGADYIIPT PFDPRLIGEI AGAVAKAAMD SGVATKTLDL EEYKRALSHQ
NTRSGQLMLP VFHAARGSQC RIVYGEGEDE RVLRAIQDGL DEGIVKPTIV ARRRLLEQKL
ADMGLSFQLD RDVEVIDPET DHDIMRELVE AYRAIAARKG VPPDEILRHV YRRPTVTAAM
LLRTGRVDAA LVGGRSEYWG QVEHVLRIID RAPNHSRIYA LSGLILDAGA LFITDTHMVP
DPTPDQIAEM TLLASTELQH FGLKPRVALL SHSNFGASNS PSARKMRAAL DLVRQAAPEL
AVDGEMHADA ALSQALRERL IPDSRFEGPA NLLVMPNLDA ANITLTALGA SSSSPTVGPM
LIGTAQPIHV LTPGVTARGI LNLTAIAAAE VAREG
//
