ID K9DBY6_9BURK Unreviewed; 316 AA.
AC K9DBY6;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Xylosidase/arabinosidase {ECO:0008006|Google:ProtNLM};
GN ORFNames=HMPREF9710_02940 {ECO:0000313|EMBL:EKU81748.1};
OS Massilia timonae CCUG 45783.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae; Telluria group; Massilia.
OX NCBI_TaxID=883126 {ECO:0000313|EMBL:EKU81748.1, ECO:0000313|Proteomes:UP000009874};
RN [1] {ECO:0000313|EMBL:EKU81748.1, ECO:0000313|Proteomes:UP000009874}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 45783 {ECO:0000313|EMBL:EKU81748.1,
RC ECO:0000313|Proteomes:UP000009874};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Huys G., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Howarth C., Imamovic A., Larimer J., McCowen C., Montmayeur A.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Massilia timonae CCUG 45783.";
RL Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKU81748.1}.
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DR EMBL; AGZI01000037; EKU81748.1; -; Genomic_DNA.
DR RefSeq; WP_005667565.1; NZ_JH992923.1.
DR AlphaFoldDB; K9DBY6; -.
DR STRING; 47229.LO55_3643; -.
DR PATRIC; fig|883126.3.peg.2967; -.
DR eggNOG; COG3507; Bacteria.
DR HOGENOM; CLU_009397_4_1_4; -.
DR OrthoDB; 9760116at2; -.
DR Proteomes; UP000009874; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR CDD; cd18618; GH43_Xsa43E-like; 1.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43772:SF2; BETA-1,4-XYLOSIDASE (EUROFUNG); 1.
DR PANTHER; PTHR43772; ENDO-1,4-BETA-XYLANASE; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00022651};
KW Reference proteome {ECO:0000313|Proteomes:UP000009874};
KW Signal {ECO:0000256|SAM:SignalP};
KW Xylan degradation {ECO:0000256|ARBA:ARBA00022651}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..316
FT /note="Xylosidase/arabinosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003926142"
FT ACT_SITE 31
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 203
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 157
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 316 AA; 34944 MW; 078D7C7A459353BD CRC64;
MMRHLPPALL FCIAAGAHAA NPIVTDIFTA DPAVVVDGKR LYLYTGRDEA SPGETTYRMN
EWRVFSTCDM RNWTDHGVAA MVKTFAWAKA DAFAADVVRH KGKYYLYAPV KHATIPGFAI
GVGVSDTPTG PFRDARGSAL VTNDMTRETP NGWDDIDPAV FVDDDGQAYM YFGNKVLKYV
RLKPNMIELD GTIRTVGLFN FEEAPWLHKR DGVYYLSYAS GLPEVIAYAT GPSPTGPWTY
RGVIMDKNKV THTIHQAFAD FNGKSYVFYH NEMVSNADYR RSVAVEEFSY GKDGAIPRIA
QTRAGPAANP APGCNN
//
