ID K9Y089_STAC7 Unreviewed; 346 AA.
AC K9Y089;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=3-isopropylmalate dehydrogenase {ECO:0000256|ARBA:ARBA00019276};
DE EC=1.1.1.85 {ECO:0000256|ARBA:ARBA00013101};
DE AltName: Full=3-IPM-DH {ECO:0000256|ARBA:ARBA00033138};
DE AltName: Full=Beta-IPM dehydrogenase {ECO:0000256|ARBA:ARBA00030010};
GN OrderedLocusNames=Sta7437_4332 {ECO:0000313|EMBL:AFZ37801.1};
OS Stanieria cyanosphaera (strain ATCC 29371 / PCC 7437).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Pleurocapsales; Dermocarpellaceae;
OC Stanieria.
OX NCBI_TaxID=111780 {ECO:0000313|EMBL:AFZ37801.1, ECO:0000313|Proteomes:UP000010473};
RN [1] {ECO:0000313|Proteomes:UP000010473}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29371 / PCC 7437 {ECO:0000313|Proteomes:UP000010473};
RX PubMed=23277585; DOI=10.1073/pnas.1217107110;
RA Shih P.M., Wu D., Latifi A., Axen S.D., Fewer D.P., Talla E., Calteau A.,
RA Cai F., Tandeau de Marsac N., Rippka R., Herdman M., Sivonen K.,
RA Coursin T., Laurent T., Goodwin L., Nolan M., Davenport K.W., Han C.S.,
RA Rubin E.M., Eisen J.A., Woyke T., Gugger M., Kerfeld C.A.;
RT "Improving the coverage of the cyanobacterial phylum using diversity-driven
RT genome sequencing.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:1053-1058(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate
CC + CO2 + NADH; Xref=Rhea:RHEA:32271, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17865, ChEBI:CHEBI:35121, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.1.1.85;
CC Evidence={ECO:0000256|ARBA:ARBA00000624};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 3/4.
CC {ECO:0000256|ARBA:ARBA00004762}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC dehydrogenases family. LeuB type 1 subfamily.
CC {ECO:0000256|ARBA:ARBA00008319}.
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DR EMBL; CP003653; AFZ37801.1; -; Genomic_DNA.
DR RefSeq; WP_015195455.1; NC_019748.1.
DR AlphaFoldDB; K9Y089; -.
DR STRING; 111780.Sta7437_4332; -.
DR KEGG; scs:Sta7437_4332; -.
DR PATRIC; fig|111780.3.peg.4486; -.
DR eggNOG; COG0473; Bacteria.
DR HOGENOM; CLU_031953_0_3_3; -.
DR OrthoDB; 9806254at2; -.
DR UniPathway; UPA00048; UER00072.
DR Proteomes; UP000010473; Chromosome.
DR GO; GO:0003862; F:3-isopropylmalate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.718.10; Isopropylmalate Dehydrogenase; 1.
DR InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR InterPro; IPR004429; Isopropylmalate_DH.
DR PANTHER; PTHR42979; 3-ISOPROPYLMALATE DEHYDROGENASE; 1.
DR PANTHER; PTHR42979:SF1; 3-ISOPROPYLMALATE DEHYDROGENASE; 1.
DR Pfam; PF00180; Iso_dh; 1.
DR SMART; SM01329; Iso_dh; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR PROSITE; PS00470; IDH_IMDH; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304};
KW Leucine biosynthesis {ECO:0000256|ARBA:ARBA00022430};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:AFZ37801.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000010473}.
FT DOMAIN 6..340
FT /note="Isopropylmalate dehydrogenase-like"
FT /evidence="ECO:0000259|SMART:SM01329"
SQ SEQUENCE 346 AA; 38188 MW; 52521D75FAA368BF CRC64;
MKKEYQLTVL PGEGIGTEVV DAAVLVLQEL AKIYQFSLDI NYGLIGKSAY QQLGNYFPEL
TAKLCQNSDG ILFGAVSQGG LLELRKRFDF YLNVRPVRPL LSLLDKSSLK AEKLIGVDLL
FVRELVSGIY FGAAGRGKDE QGDYGYHTMK YYDWEIKRIA RFALQKAQQR KKLLTVAHKQ
NALPHLPWSA LVKAEAHNFP DVKIEPMLVD TLAMQLVINP QHFDVILAGN LFGDILSDLG
GALVGSIGLL GSASINANGF GMYEPVHGTA PDLAGKGIAN PLGTFNAVGL MLQQWGENQA
AQKLIQTQEN LLAKGYRTAD LYSQTESEIL VNTKTFTNLF IQEMYQ
//
